Abstract
Cystic Echinococcosis/Hydatidosis considered as one of the most important parasitic diseases in humans and animals across the world. The goal of the present study was to determine a native antigen with an acceptable sensitivity and specificity to be used in the human hydatid cyst diagnostic methods. In the present study, recombinant P29 antigen was used to detect the antibodies in the serum of patients with hydatid cysts of the liver. In fact, purified recombinant P29 protein is used as an antigen in ELISA. In order to evaluate the recombinant P29 protein for diagnostic ELISA, 25 serums obtained from people harboring the hydatid cysts were tested. The result of the gene expression on a 12% SDS-PAGE gel showed a band with a length of 28 KD. Also, 28KDa band was observed through the reaction of recombinant P29 protein with Anti T7-tag monoclonal antibody in the western blotting method. This protein showed satisfactory results in detecting the hydatid cyst antibodies in the serum of patients having hydatid cysts. Twenty two of 25 hydatidosis serums positively reacted in ELISA using with P29 protein, indicating in 92% of ELISA sensitivity, 95% of specificity, 95.83% of positive predictive value, and 90.42% of negative predictive value for recombinant P29 protein. Whereas the produced recombinant protein P29 showed promising results in the diagnosis of hydatidosis but of more research needs to be done to reach a more accurate conclusion.
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Abbreviations
- ELISA:
-
Enzyme-linked immuno-sorbent assay
- MSC:
-
Multiple cloning site
- PCR:
-
Polymerase chain reaction
- PBST:
-
Phosphate buffered saline with Tween 20
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This paper is a part of Ph.D. thesis with code 234 of the first author and financially supported by Science and Research Branch of Islamic Azad University, Tehran, Iran.
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ZM designed the study; ZM performed the experiments. All the authors discussed the results and commented on the final manuscript.
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Masih, Z., Hoghooghirad, N., Madani, R. et al. Expression and production of protoscolex recombinant P29 protein and its serological evaluation for diagnosis of human hydatidosis. J Parasit Dis 46, 377–383 (2022). https://doi.org/10.1007/s12639-021-01454-2
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DOI: https://doi.org/10.1007/s12639-021-01454-2