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Transplacental transmission of Theileria annulata in cattle confirmed by molecular techniques

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Abstract

Theileria annulata (T. annulata) is a tick-borne apicomplexan parasite affecting ruminants and causes severe economic loss. The present paper reports the vertical transmission of T. annulata in a three day old crossbred Jersey calf. Molecular diagnosis of tropical theileriosis was done by PCR, cloning, sequencing and phylogenetic analysis of isolated parasites. Calf showed very low hematocrit value (HCT) (17.82%), red blood cells count (RBC) count (6.9 × 106/μL), and hemoglobin concentration (Hb) (6.6 g/dL). Phylogenetic analysis of Tams 1 gene showed that T. annulata Khorda isolate (MW123091) shared 99.23%, 99.23% and 99.11% nucleotide homology with Puri, India (MN818858), Bahrain (AF214797) and Hyderabad, India (MK034702), respectively. This is the first study in Odisha, India to give an insight into the molecular detection, phylogeny and hematological analysis of T. annulata infected crossbred Jersey calf which got the infection through transplacental transmission.

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Authors and Affiliations

  1. Department of Clinical Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar, 751003, Odisha, India

    Ahmed Magdy Selim, Manoranjan Das, Santosh Kumar Senapati & Geeta Rani Jena

  2. Internal Medicine, Infectious Diseases and Fish Diseases Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt

    Ahmed Magdy Selim

  3. Department of Animal Breeding and Genetics, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar, 751003, Odisha, India

    Chinmoy Mishra

  4. Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar, 751003, Odisha, India

    Bijayendranath Mohanty

  5. Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneshwar, 751003, Odisha, India

    Susen Kumar Panda

  6. Ministry of Health and Population, Damietta, Egypt

    Al shymaa Al sayed Shwareb

Authors
  1. Ahmed Magdy Selim
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  2. Manoranjan Das
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  3. Santosh Kumar Senapati
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  4. Geeta Rani Jena
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  5. Chinmoy Mishra
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  6. Bijayendranath Mohanty
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  7. Susen Kumar Panda
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  8. Al shymaa Al sayed Shwareb
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Correspondence to Ahmed Magdy Selim.

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In this study, 3 ml of blood from the jugular vein was obtained from calf under aseptic conditions after obtaining permission from the farm owners.

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Selim, A.M., Das, M., Senapati, S.K. et al. Transplacental transmission of Theileria annulata in cattle confirmed by molecular techniques. J Parasit Dis 45, 336–340 (2021). https://doi.org/10.1007/s12639-021-01365-2

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  • DOI: https://doi.org/10.1007/s12639-021-01365-2

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