Abstract
Reinforcement of the body with exogenous antioxidants have been shown to mitigate the negative effects of African trypanosomiasis on the host and contribute greatly to their survival. This study was therefore conducted to evaluate the effects of oral administration of Vitamin E on the early stage of Trypanosoma brucei brucei infection. To achieve this, parasite free healthy rats were acclimatized for 2 weeks before they were divided into three groups. Two of the groups were infected by intraperitoneal inoculation of 1 × 104 parasites/rat and monitored for the presence of Trypanosoma brucei brucei. Blood samples were collected from the infected rats from the second day post infection to detect the presence of parasites. Vitamin E treatment started day 4 post infection at the onset of parasitaemia. Parasites were monitored till the end of the study. The blood glucose level was determined using a glucometer; the lipid profile, liver and kidney biomarkers, electrolytes and protein were determined by colorimetric method using commercial kits. Haematological parameters were analysed using a sysmex haematology analyser. The results of this study showed that the infection adversely affected the biomarkers examined showing its negative effect on liver, kidney, haematological parameters and host electrolyte balance. Treatments with Vitamin E was however able to mitigate the negative effect of this infection. In conclusion, the treatment was able to ameliorate the anaemia and organ damage caused by Trypanosoma brucei brucei, extend the life span of the treated rats and greatly delay the time taken to get to the second stage of the infection.
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Abdulazeez MA, Ibrahim AB, Edibo ZY, Sidali JO, Idris HO (2013) Anti-trypanosomal effect of Peristrophe bicalyculataextract on Trypanosoma brucei brucei-infected rats. Asian Pac J Trop Biomed 3(7):523–531
Abubakar A, Iliyasu B, Yusuf AB, Igweh AC, Onyekwelu NA, Shamaki BA, Afolayan DO, Ogbadoyi EO (2005) Antitrypanosomal and haematological effects of selected Nigerian medicinal plants in Wistar rats. Biokemistri 17(2):95–99
Abubakar AS, Onaolapo MH, Abdullahi YA, Kabir SS, Danladi O (2016) The metabolism of African trypanosomes in relation to pathogenic mechanisms: a review. J Pharm Biol Sci 11(2):68–72
Abuessailla A, Ismail AA, Agab H, Shuaib YA (2017) Serum biochemical and histopathological changes in rats experimentally infected with trypanosoma evansi isolated from dromedary camels in Sudan. Int J Life Sci Sci Res 3(3):1075–1084. https://doi.org/10.21276/ijlssr.2017.3.3.19
Adah MI, Otesile EB, Joshua RA (1992) Changes in levels of transaminases in goats experimentally infected with Trypanosoma congolense. Revue Elev Med Vet Pays Trop 45:284–286
Adam S, Barde N, Abenga JN, Useh NM, Ibrahim NDG, Esievo KAN (2009) Experimental Trypanosoma brucei infection-induced changes in the serum profiles of lipids and cholesterol and the clinical implications in pigs. J Cell Anim Biol 3(2):15–20
Adamu S, Ige AA, Jatau ID, Neils JS, Useh NM, Bisalla M et al (2008) Changes in the serum profiles of lipids and cholesterol in sheep experimental model of acute African trypanosomosis. Afr J Biotechnol 7(12):2090–2098
Adamu O, Haruna MK, Ovbagbedia RP, Uzowuru M, Eyitope MG, Anichebe F (2015) Effects of immunace and iron dextran on anemia and immunosuppression of T. Brucei infected rats. Int. J. Curr. Res. Chem. Pharm. Sci. 2(8):1–6
Adebayo AO, Ibitoroko GOM, Emechete EM (2019) Effect of vitamin E on hydrogen peroxide and nitrogen levels in male wistar albinorats infected with trypanosomaBrucei Brucei. Int J Biomed Adv Res 10(01):e4934
Adeyemi OS, Sulaiman FA (2012) Biochemical and morphological changes in Trypanosoma brucei brucei-infected rats treated with homidium chloride and diminazene aceturate. J Basic Clin Physio Pharm 23(4):179–183
Adieme IC, Ezeh IO, Ugochukwu EI, Romanus CE (2014) Effect of diminazene aceturate, levamisole and vitamin C combination therapy in rats experimentally infected with Trypanosoma brucei brucei. Asian Pac J Trop Med 7:438–445
Akanji MA, Adeyemi OS, Oguntoye SO, Sulyman F (2009) Psidium guajava extract reduces trypanosomosis associated lipid peroxidation and raises glutathione concentrations in infected animals. EXCLI J 8:148–154
Akazue PI, Ebiloma GU, Ajibola O, Isaac C, Onyekwelu K, Ezeh CO, Eze AA (2019) Sustainable elimination (Zero Cases) of sleeping sickness: how far are we from achieving this goal? Pathogens 8(135):1–18
Akpa PO, Ezeokonkwo RC, Eze CA, Anene BM (2008) Comparative efficacy assessment of pentamidine isethionate and diminazene aceturate in the chemotherapy of Trypanosoma brucei brucei infection in dogs. Vet Parasitol 151:139–149
Allam L, Ogwu D, Agbede RIS, Sackey Allam AKB, Ogwu LD, Agbede RIS, Sackey AKB (2011) Hematological and serum biochemical changes in gilts experimentally infected with Trypanosoma brucei. J Vet Arhiv 81(5):597–609
Arora JK, Pathok KML (1995) Clinico-haematological and biochemical changes associated with T. evansi infection in dogs. Indian J Anim Health 34(1):33–38
Bala AY, Adamu T, Abubakar U, Ladan MJ (2012) Amelioration of trypanosome-infection-induced alterations in serum cholesterol, triglycerides and proteins by hydro-ethanolic extract of Waltheria indica in rats. Res J Parasitol 6:127–135
Bouteille B, Buguet A (2012) The detection and treatment of human African trypanosomiasis. Res Rep Trop Med 3:35–45
Bruijn JA, Oemar BS, Ehrich JH, Foidart JM, Fleuren GJ (1987) Anti-basement membrane glomerulopathy in experimental trypanosomiasis. J Immunol 139(7):2482–2488
Capewell P, Cooper A, Clucas C, Weir W, Macleod A (2014) A co-evolutionary arms race: trypanosomes shaping the human genome, humans shaping the trypanosome genome. Parasitology 142(S1):1-12. https://doi.org/10.1017/S0031182014000602
Cnops J, Magez S, De Trez C (2015) Escape mechanisms of African trypanosomes: why trypanosomosis is keeping us awake. Parasitology 142(3):417–427
Creek DJ, Mazet M, Achcar F, Anderson J, Kim DH, Kamour R, Morand P, Millerioux Y, Biran M, Kerkhoven EJ, Chokkathukalam A (2015) Probing the metabolic network in bloodstream-form Trypanosoma brucei using untargeted metabolomics with stable isotope labelled glucose. PLoS Pathog 11(3):e1004689
Edoga CO, Njoku OO, Okeke JJ, Ani CE (2013) Effect of vitamin C treatment on serum protein, albumin, beta globulin profiles and body weight of Trypanosoma Brucei-infected Rattus Norvegicus. Anim Res Int 10(1):1685–1688
Ekanem JT, Yusuf OK (2008) Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. brucei-infected rats. Afr J Biomed Res 11:79–85
Eze JI, Okeke MC, Ngene AA, Omeje JN, Abonyi FO (2013) Effects of dietary selenium supplementation on parasitemia, anemia and serum proteins of Trypanosoma brucei brucei infected rats. Exp Parasitol 135:331–336
Ezeokonkwo RC, Ezeh IO, Onunkwo JI, Onyenwe IW, Iheagwam CN, Agu WE (2012) Comparative serum biochemical changes in mongrel dogs following single and mixed infections of Trypanosoma congolense and Trypanosoma brucei brucei. Vet Parasitol 190(1–2):56–61
Fiennes RTW, Jones ER, Laws SG (1946) The course and pathology of T. congolense (brooden) disease of cattle. J Comp Pathol 56:1–27
Franco JR, Cecchi G, Priotto G, Paone M, Diarra A, Grout L, Simarro PP, Zhao W, Argaw D (2018) Monitoring the elimination of human African trypanosomiasis: update to 2016. PLOS Neglect Trop Dis 12(12):e0006890
Gaithuma AK, Yamagishi J, Martinelli A, Hayashida K, Kawai N, Marsela M, Sugimoto C (2019) A single test approach for accurate and sensitive detection and taxonomic characterization of Trypanosomes by comprehensive analysis of internal transcribed spacer 1 amplicons. PLoS Neglect Trop Dis 13(2):e0006842
Gjini E, Haydon DT, Barry JD, Cobbold CA (2010) Critical interplay between parasitesdifferentiation, host immunity, and antigenic variation in trypanosome infections. Am Nat 176(4):424–439
Gutteridge JMC (1995) Lipid peroxidation and antioxidants as biomarkers of tissue damage. Clin Chem 41:1819–1828
Herrera E, Barbas C (2001) Vitamin E, action, metabolism and perspectives. J Physiol Biochem 57:43–56
Herbert WJ, Lumsden WH (1976) Trypanasoma brucei: a rapid matching method for estimating the host's parasitaemia. Exper Parasitol 40:427–431
Hussain R, Khan A, Abbas RZ, Ghaffar A, Abbas G, Rahman T, Ali F (2016) Clinico-hematological and biochemical studies on naturally infected camels with trypanosomiasis. Pak J Zool Soc 48(2):311–316
Igbokwe IO (1994) Mechanisms of cellular injury in African trypanosomiasis. Vet Bull 64:611–620
Igbokwe IO, Muhammed A (1992) Some plasma biochemical changes in experimental Trypanosoma brucei infection in Sokoto red goats. Rev Elev Med Pays Trop 45(3–40):287–290
Igbokwe IO, Umar IA, Omage JJ, Ibrahim NDG, Kadima KB, Obagaiye OK, Saror DI, Esievo KAN (1996) Effect of acute Trypanosoma vivax infection on cattle erythrocyte glutathione and susceptibility to in vitro peroxidation. Vet Parasitol 63:215–224
Ismaila AU, Zipporah AT, Funnilayo II, Lawan AG, BB, (2000) The role of vitamin C administration in alleviation of organ damage in rats infected with Trypanosoma brucei. Clin Biochem Nutr 28:1–7
Kaneko JJ, Harvey JW, Bruss LM (2008) Clinical biochemistry of domestic animals, 6th edn. Elsevier, Hoboken, pp 854–858
Kapoor S (2011) Hepascore: a new liver function test. J Clin Diagnos Res 5(1):155–156
Karaye GP, Sackey AKB, Tekdek LB, Lawan IA (2017) Biochemical response of red sokoto bucks experimentally infected with Trypanosoma congolense and Trypanosoma bruceitreatment and relapse. Saudi J Biomed Res 2(4):86–90
Katunguka-Rwakishaya E, Murray M, Holmes PH (1999) The influence of energy intake on some blood biochemical parameters in Scottish Blackface sheep infected with Trypanosoma congolense. Vet Parasitol 84:1–11
Kobo PI, Ayo JO, Tagang Aluwong T, Zezi AU, Maikai VA (2013) Haematological changes in Trypanosoma brucei brucei infected wistar rats treated with a flavonoid mixture and/or diminazene aceturate. Biol Med J 6:213. https://doi.org/10.4172/0974-8369.1000213
Longdet IY, Achemu HU, Okanlawon CN (2014) Potentials of methanolic extract of N. latifolia stem bark against T. congolense infection in experimental rats. J Agric Sci Policy Res 4:29–41
Malvy D, Chappuis F (2011) Sleeping sickness. Clin Microbiol Infect 17:986–995
Mazet M, Morand P, Biran M, Bouyssou G, Courtois P, Daulouède S, Millerioux Y, Franconi JM, Vincendeau P, Moreau P, Bringaud F (2013) Revisiting the central metabolism of the blood stream forms of Trypanosoma brucei: Production of acetate in the mitochondrion Is essential for parasiteviability. PLoS Neglect Trop Dis 7(12):e2587
Mbaya AW, Aliyu MM, Nwosu CO, Egbe-Nwiyi T (2009) The relationship between parasitaemia and anaemia in concurrent Trypanosoma brucei and Haemonchus contortus infections in red fronted gazelles (Gazella rufifrons). Vet Arhiv 79(5):451–460
Mishra RR, Senapati SK, Sahoo SC, Das MR, Sahoo G, Patra RC (2017) Trypanosomiasis induced oxidative stress and hemato-biochemical alteration in cattle. J Entomol Zool Stud 5(6):721–727
Morrison WI, Murray M, Sayer PD, Preston JM (1981) The pathogenesis of experimentally induced Trypanosoma brucei infection in dog. Am J Pathol 102:182–194
Ngure RM, Ndungu JM, Ngotho JM, Nancy MK, Maathai RG, Gateri LM (2008) Biochemical changes in the plasma of vervet monkeys (Chlorocebus aethiops) experimentally infected with Trypanosoma brucei rhodesiense. J Cell Anim Biol 2(7):150–157
Nwagwu M, Opperdoes FR (1982) Regulation of glycolysis in Trypanosoma brucei: hexokinase and phosphofructokinase activity. Acta Trop 39:61–72
Omotainse SO, Anosa VO (1995) Leucocyte and thrombocyte response in dogs experimentally infected with Trypanosoma brucei. Rev Elev Med Vet Pays Trop 48(3):254–258
Ogunsanmi AO, Taiwo VO (2001) Pathobiochemical mechanisms involved in the control of the disease caused by Trypanosoma congolense in African grey duiker (Sylvicapra grimmia). Vet Parasitol 96:51–63
Ogunsanmi AO, Akpavie SO, Anosa VO (1994) Serum biochemical changes in West African Dwarf sheep experimentally infected with Trypanosoma brucei. Revue d’Elevage et de medicine veterinaire des pays tropicaux 47(2):195–200
Oparah NQ, Sackey KBA, Lawal AI, Abdullahi SU (2017) Haematological indices in Trypanosoma brucei brucei (Federe isolate) infected Nigerian donkeys (Equus asinus) treated with homidium and isometamidium chloride. Mac Vet Rev 40(1):73–82. https://doi.org/10.1515/macvetrev-2017-0014
Opperdoes FR, Hart DR, Baudhain P (1986) Biogenesis of glycosome (microbodies) in the trypanosomatidae T. brucei. Eur J Cell Biol 41:30
Orhue N, Nwanze E, Okafor A (2005) Serum total protein, albumin and globulin levels in Trypanosoma brucei-infected rabbits: effect of orally administered Scoparia dulcis. Afr J Biotechnol 4:1152–1155
Sackey AKB (2011) Hematological and serum biochemical changes in gilts experimentally infected with Trypanosoma brucei. J Vet Arhiv 81(5):597–609
Saleh MA, Bassam MA, Sanousi SA (2009) Oxidative stress in blood of camels (Camelus dromedaries) naturally infected with Trypanosoma evansi. Vet Parasitol 162:192–199
Samia HA, Elmalik KH, Khalid HS, Shamat AMA, Khojali SME (2004) Biochemical changes in rats experimentally infected with T. evansi. J Anim Vet Adv 3(7):483–486
Samuel FU, Adamu S, Bisalla M, Chiezey NP, Mohammed AK, Bello TK et al (2016) Effect of T. congolense on haematological parameters in experimentally infected donkeys. J Anim Prod Res 28(1):14–24
Sazmand A, Rasooli A, Nouri M, Hamidinejat H, Hekmatimoghaddam S (2011) Serobiochemical alterations in subclinically affected dromedary camels with Trypanosoma evansi in Iran. Pak Vet J 31(3):223–226
Seyfang A, Duszenko M (1991) Specificity of glucose transport in Trypanosoma brucei. Effective inhibition by phloretin and cytochalasin B. Eur J Biochem 202:191–196
Simarro PP, Cecchi G, Franco JR, Paone M, Diarra A, Ruiz-Postigo JA, Fèvre EM, Mattioli RC, Jannin JG (2012) Estimating and mapping the population at risk of sleeping sickness. PLoS Neglect Trop Dis 6:e1859
Smith TK, Bringaud F, Nolan DP, Figueiredo LM (2017) Metabolic reprogramming during the Trypanosoma bruceilife cycle. F1000Research 6:683
Sow A, Sidibé I, Kalandi M, Bathily A, Ndiaye NP, Ouédraogo M, Mouiche MM, Sawadogo GJ (2014) Biochemical changes induced by natural infection of trypanosomosis in Burkinabese local donkey breeds. Comp Clin Pathol 23:103–109
Stijlemans B, Radwanska M, De Trez C, Magez S (2017) African trypanosomes undermine humoral responses and vaccine development: link with inflammatory responses? Front Immunol 8:582
Sulaiman AF, Akanji MA, Yakubu MT (2012) Effect of administration of Ibuprofen on the levels of parasitaemia, albumin and total protein concentration in rats infected with Trypanosoma bruce brucei. Afr Sci 13(1):57–63
Tabel H, Wei G, Shi M (2008) T cells and immunopathogenesis of experimental African trypanosomiasis. Immunol Rev 225:128–139
Takeet MI, Fagbemi BO (2009) Haematological, pathological and plasma biochemical changes in rabbits experimentally infected with Trypanosoma congolense. Sci World J 4(2)
Taylor K, Authie EM (2004) Pathogenesis of animal trypanosomiasis. In: Maudlin I, Holmes PH, Miles MA (eds) The trypanosomiasis. CAB International, London, pp 331–353
Tesfaye D, Speybroeck N, De Deken R, Thys E (2012) Economic burden of bovine trypanosomosis in three villages of Metekel zone, northwest Ethiopia. Trop Anim Health Prod 44(4):873–879
Traber MG, Atkinson J (2007) Vitamin E, antioxidant and nothing more. Free Radic Biol Med 43:4–15
Trindade S, Rijo-Ferreira F, Carvalho T, Pinto-Neves D, Guegan F, Aresta-Branco F, Bento F, Young SA, Pinto A, Van Den Abbeele J, Ribeiro RM (2016) Trypanosoma brucei parasites occupy and functionally adapt to the adipose tissue in mice. Cell Host Microbe 19(6):837–848
Ukpai OM, Nwabuko OP (2014) Effects of Trypanosoma brucei brucei on haematological parameters and pathology of internal organs of Trypanosoma brucei brucei infected albino rats. Niger J Biotechnol 27:8–13
Umar IA, Wuro-Chekke AU, Gidado A, Igbokwe IO (1999) Effects of Combined parental vitamin C and E administration on the severity of anaemia, hepatic and renal damage in T. brucei-infected rabbits. Vet Parasitol 85:43–47
Umar IA, Toh ZA, Igbalajobi FI, Igbokwe IO, Gidado A (1999) The effect of orally administered vitamins C and E on the severity of anaemia in T. brucei-infected rats. Trop Vet 18:71–77
Umar IA, Toh ZA, Igbalajobi FI, Igbokwe IO, Gidado A (2000) The effects of orally administered vitamins C and E on severity of anaemia in Trypanosoma brucei- infected rats. Trop Vet 18:71–77
Umar IA, Toh ZA, Igbalagobi FI, Gidado A, Buratai LB (2000) The role of vitamin C administration in alleviation of organ damage in rats infected with the T. brucei. J Clin Biochem Nutr 28:1–7
Umar IA, Igbalajobi FI, Toh ZA, Gidado A, Shugaba A et al (2001) Effect of repeated daily doses of vitamin E (alpha-tocopherol) on some biochemical indices of rats infected with T. brucei (Basa strain). West Afr J BiolSci 12:1–7
Umar IA, Igbalajobi FI, Toh ZA, Gidado A, Shugaba A, Buratai LB (2001) Effects of oral administration of repeated doses of vitamin E (alpha tocopherol) on some biochemical indices in rats infected with T. b. brucei. West Afr J Biol Sci 12:1–7
Umar IA, Ogenyi E, Okodaso D, Kimeng E, Stancheva GI, Omage JJ, Isah S, Ibrahim MA (2007) Amelioration of anaemia and organ damage by combined intraperitoneal administration of vitamins A and C to trypanosoma brucei brucei-infected rats. Afr J Biotechnol 6(18):2083–2086
Umar IA, Rumah BL, Bulus SL, Kamla AA, Jobin A, Asueliman BI, Mazai MH, Ibrahim MA, Isah S (2008) Effects of intraperitoneal administration of vitamins C and E or A and E combinations on the severity of Trypanosoma brucei brucei infection in rats. Afri J Biochem Res 2(3):088–091
Van Hellemond JJ, Tielens AGM (2006) Adaptations in the lipid metabolism of the protozoan parasite Trypanosoma brucei. FEBS Lett 580:5552–5558
Vickerman K, Tetley L (1979) Biology and ultrastructure of trypanonomes in relation to pathogenesis. In: Losos G, Chouinard A (eds) Pathogenicity of trypanosomes. IDRC, Ottawa, pp 23–31
Vray B, Debaetselier P, Ouaisis A, Carlier Y (1991) Trypanosoma with T. evansi infection in dogs. Indian J Anim Health 34(1):33–38
Yusuf AB, Umar IA, Nok AJ (2012) Effects of methanol extract of Vernonia amygdalina leaf on survival and some biochemical parameters in acute Trypanosoma brucei brucei infection. Afr J Biochem Res 6(12):150–158
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Ojo, R.J., Enoch, G.A., Adeh, F.S. et al. Comprehensive analysis of oral administration of Vitamin E on the early stage of Trypanosoma brucei brucei infection. J Parasit Dis 45, 512–523 (2021). https://doi.org/10.1007/s12639-020-01322-5
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DOI: https://doi.org/10.1007/s12639-020-01322-5