Abstract
The present study reports the characteristics of the 5S rRNA spacer sequences of Dirofilaria repens microfilariae isolated from dogs. The 22 nucleotide long spliced leader 1 sequences located in the 5S rRNA spacer region are completely conserved in all nematodes. There is variation in the spliced leader 1 sequences and associated sites in the 5S rRNA spacer region of D. repens. Absence of canonical SL 1 sequences distinguishes D. repens from other filarial species.
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Acknowledgements
The present paper is a part of Ph.D. thesis submitted to the Kerala Veterinary and Animal Sciences University and the authors are thankful to the University for the Facilities and grant rendered for the research.
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Deepa Chirayath—performed the research work as a part of Ph.D. and prepared the manuscript. Alex P. C.—major guide for the Ph.D. Research. Usha Narayana Pillai—minor guide for the Ph.D. Research
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There is no separate funding for this research project. The project is formulated following the guidelines of Institutional Animal Ethics Committee and got approval from faculty Research Committee. All biomaterials required for the research were collected with prior consent of the owners of the animals. There is no conflict of interest for the research findings.
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Chirayath, D., Alex, P.C. & Pillai, U.N. Molecular characterization of 5S ribosomal spacer sequences of Dirofilaria repens microfilariae in dogs in Kerala, India. J Parasit Dis 44, 864–868 (2020). https://doi.org/10.1007/s12639-020-01257-x
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DOI: https://doi.org/10.1007/s12639-020-01257-x