Abstract
Taenia solium cysticercosis is a neglected zoonosis prevalent in developing countries including India. The disease has a substantial health impact in India. The control of this disease in pigs would benefit both veterinary and public health. In view of this, the current study was designed to know the effectiveness of oxfendazole against T. solium in pigs reared in Punjab, India. A randomized controlled trail was designed with two groups of treatment (T1 and T2) and a control group. Pigs from ten different litters were selected. Pigs (4 months of age) from the same litter were randomly assigned into these three groups. Treatment 1 (T1) group pigs received oxfendazole@30 mg/kg body weight at 4 months of age, treatment 2 (T2) received oxfendazole@30 mg/kg body weight treatment at 9 months of age and the control group (C) received placebo at 4 months of age. Blood samples were collected at three intervals at the age of 4, 9 and 12 months. Sandwich antigenic ELISA was performed to determine the serological status of disease in pigs. Three piglets in treatment 1 were found positive at 4 months but were recorded seronegative in subsequent samplings. One pig in the control group was positive at 9 and 12 months of age. Results showed promising effects of oxfendazole for the control of T. solium cysticercosis in pigs of Punjab, India.
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An G, Murry DJ, Gajurel K, Bach T, Deye G, Stebounova LV, Codd EE, Horton J, Gonzalez AE, Garcia HH, Ince D, Hodgson-Zingman D, Nomicos E, Conrad T, Kennedy J, Jones W, Gilman RH, Winokur P (2019) Pharmacokinetics, safety, and tolerability of oxfendazole in healthy volunteers: a randomized, placebo-controlled first-in-human single-dose escalation study. Antimicrob Agents Chemother 63(4):e02255–e022518. https://doi.org/10.1128/AAC.02255-18
Chawhan P, Singh B, Sharma R, Gill PS (2015) Prevalence and molecular epidemiology of porcine cysticercosis in naturally infected pigs (Sus scrofa) in Punjab, India. Rev Sci Technol OIE 34(3):953–960
Chowdhury N, Saleque A, Sood NK and Singla LD (2014) Induced neurocysticercosis in Rhesus monkeys (Macaca mulatta) produces clinical signs and lesions similar to natural disease in man. Sci World J, Article ID 248049, p 5
Garcia HH, Gonzalez AE, Tsang VC, O’Neal SE, Llanos-Zavalaga F, Gonzalvez G, Romero J, Rodriguez S, Moyano LM, Ayvar V, Diaz A, Hightower A, Craig PS, Lightowlers MW, Gauci CG, Leontsini E, Gilman RH, Cysticercosis Working Group in Peru (2016) Elimination of Taenia solium transmission in Northern Peru. N Engl J Med 374(24):2335–2344. https://doi.org/10.1056/nejmoa1515520
Goel D, Dhanai JS, Agarwal A, Mehlotra V, Saxena V (2011) Neurocysticercosis and its impact on crude prevalence rate of epilepsy in an Indian community. Neurol India 59(1):37
Gonzalez AE, Gavidia C, Falcon N, Bernal T, Verastegui M, Garcia HH, Gilman RH, Tsang VC, Cysticercosis Working Group in Peru (2001) Protection of pigs with cysticercosis from further infections after treatment with oxfendazole. Am J Trop Med Hyg 65(1):15–18
Khatkar MS, Dhand NK (2014) Statulator: an online tool for basic statistical analysis. http://statulator.com/
Kumar A, Mandal A, Sinha S, Singh A, Das RR (2017) Prevalence, response to cysticidal therapy, and risk factors for persistent seizure in Indian children with neurocysticercosis. Int J Pediatr. https://doi.org/10.1155/2017/8983958
Mahajan RC (1982) Geographical distribution of cysticercosis. In: Flisser A, Willms K, Laclette JP, Larralde C, Ridaura C, Beltran F (eds) Cysticercosis: present state of knowledge and perspectives. Academic Press, New York, pp 39–46
O’Neal SE, Moyano LM, Ayvar V, Rodriguez S, Gavidia C, Wilkins PP, Gilman RH, Garcia HH, Gonzalez AE (2014) Ring-screening to control endemic transmission of Taenia solium. PLoS Negl Trop Dis 8(9):e3125
Pondja A, Neves L, Mlangwa J, Afonso S, Fafetine J, Willingham AL III, Thamsborg SM, Johansen MV (2012) Use of oxfendazole to control porcine cysticercosis in a high-endemic area of Mozambique. PLoS Negl Trop Dis 6(5):e1651. https://doi.org/10.1371/journal.pntd.0001651
Prasad KN, Prasad A, Verma A, Singh AK (2008) Human cysticercosis and Indian scenario: a review. J Biosc 33:571–582
Sánchez-Torres NY, Bobadilla JR, Laclette JP, José MV (2019) How to eliminate taeniasis/cysticercosis: porcine vaccination and human chemotherapy (Part 2). Theor Biol Med Model 16(1):4. https://doi.org/10.1186/s12976-019-0100-x
Sarti E, Rajshekar V (2003) Measures for the prevention and control of Taenia solium taeniosis and cysticercosis. Acta Trop 87:137–143
Singh BB, Khatkar MS, Gill JPS, Dhand NK (2016) Estimation of the health and economic burden of neurocysticercosis in India. Acta Trop 165:161–169
Singh SP, Singh BB, Kalambhe DG, Pathak D, Aulakh RS, Dhand NK (2018) Prevalence and distribution of Taenia solium cysticercosis in naturally infected pigs in Punjab, India. PLoS Negl Trop Dis 12(11):e0006960
R Core Team (2015) R: a language and environment for statistical computing. R foundation for statistical computing, Vienna, Austria. https://www.R-project.org/
Thomas LF, de Glanville WA, Cook EAJ, Bronsvoort BMDC, Handel I, Wamae N, Kariuki S, Fevre EM (2017) Modelling the risk of Taenia solium exposure from pork produced in Western Kenya. PLOS Negl Trop Dis 11:e0005371
WHO (2005) WHO/FAO/OIE guidelines for the surveillance, prevention and control of taeniosis/cysticercosis/editor: KD Murrell; associate editors: P. Dorny et al. Paris: World Organisation for Animal Health. https://apps.who.int/iris/handle/10665/43291
Acknowledgements
The authors wish to thank Indian Council for Agricultural research for funding this research through “Outreach programme on zoonotic disease investigations”. The help received from pig farmers is also thankfully acknowledged.
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Informed consent was provided by the pig owners for the conduct of this study.
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This study was ethically approved by Committee for the Purpose of Control and Supervision of Experiments on Animals, Animal Welfare Division, Ministry of Environment, Forest and Climate Change, Government of India through Institutional Ethics Committee, Guru Angad Dev Veterinary & Animal Sciences University, Ludhiana (70th meeting F. No. 25/4/2019—CPCSEA dated 20-03-2017). Naturally reared pigs were involved in this study.
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Megha, G.K., Aulakh, R.S. & Singh, B.B. Effect of oxfendazole to control Taenia solium cysticercosis in pigs in Punjab state of India. J Parasit Dis 44, 553–558 (2020). https://doi.org/10.1007/s12639-020-01228-2
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DOI: https://doi.org/10.1007/s12639-020-01228-2