Skip to main content
SpringerLink
Log in

Phylogenetic analysis of goat warble fly (Przhevalskiana silenus) based on mitochondrial COI gene

  • Original Article
  • Published:
Journal of Parasitic Diseases Aims and scope Submit manuscript

Abstract

The larvae of the genus Przhevalskiana (Diptera: Oestridae) are the causative agents of subcutaneous myiasis in goats. Several species have been grouped under this genus based on the morphology of different larval stages, albeit with a lot of uncertainties. Thus, application of genetic tools seems to be helpful for taxonomy. During this study, the cytochrome oxidase I (COI) gene was targeted for the characterization of larval stages of goat warble fly. Fragments of 606 bp were amplified for all the specimens. Based on the COI gene analysis, all the recovered specimens were identified as larvae of Przhevalskiana silenus. Molecular data on the genus is relatively rare but present isolates revealed about 87–89% identity with previous isolates of P. silenus. According to the phylogenetic data, the present isolates branched (as a sister group) with a number of Hypoderma spp. including H. bovis, H. diana, H. lineatum and H. sinense. The present findings confirmed that the COI gene could be a suitable marker for genetic characterization and identification of larvae up to the species level.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Le Riche P, Jorgensen R, Campbell J, Efstathiou G (1973) Notes on the goat warble fly, Przhevalskiana aegagri Brauer, 1863, in Cyprus. J Nat Hist 7:615622

    Article  Google Scholar 

  • Lunt DH, Zhang DX, Szymura JM, Hewltt OM (1996) The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 5:153–165

    Article  CAS  PubMed  Google Scholar 

  • Madel G (1969) The goat warble-fly Przhevalskiana silenus in Afghanistan. Angew Parasitol 10:204–210

    Google Scholar 

  • Oryan A, Bahrami S (2012) Pathology of natural Przhevalskiana silenus infestation in goats. Trop Biomed 29:524–531

    CAS  PubMed  Google Scholar 

  • Otranto D, Puccini V (2000) Further evidence on the internal life cycle of Przhevalskiana silenus (Diptera, Oestridae). Vet Parasitol 88:321–328

    Article  CAS  PubMed  Google Scholar 

  • Otranto D, Traversa D (2004) Molecular evidence indicating that Przhevalskiana silenus, P. aegagri and P. crossii (Diptera, Oestridae) are one species. Acta Parasitol 49:173–176

    CAS  Google Scholar 

  • Otranto D, Tarsitano E, Giangaspero A, Puccini V (2000) Differentiation by polymerase chain reaction-restriction fragment length polymorphism of some Oestridae larvae causing myiasis. Vet Parasitol 90:305–313

    Article  CAS  PubMed  Google Scholar 

  • Otranto D, Traversa D, Guida B, Tarsitano E, Fiorente P, Stevens JR (2003) Molecular characterization of the mitochondrial cytochrome oxidase I (COI) gene of Oestridae larvae causing obligate myiasis. Med Vet Entomol 17:307–315

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  CAS  PubMed  Google Scholar 

  • Tamura K, Stecher G, Peterson D, Peterson Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Xiong B, Kochner TD (1991) Comparison of mitochondrial DNA sequences of seven morphospecies of black flies (Diptera: Simuliidae). Genome 34:306–311

    Article  CAS  PubMed  Google Scholar 

  • Zhang DX, Hewitt GM (1997) Assessment of the universality and utility of a set of conserved mitochondrial COI primers in insects. Insect Mol Biol 6:143–150

    Article  CAS  PubMed  Google Scholar 

  • Zhang DX, Szymura JM, Hewitt GM (1995) Evolution and structural conservation of the control region of insect mitochondrial DNA. J Mol Evol 40:382–391

    Article  CAS  PubMed  Google Scholar 

  • Zumpt F (1965) Myiasis in man and animals of the old world. Butterworths, London, pp 205–214

    Google Scholar 

Download references

Acknowledgements

The authors thank Mr. Mootabi Alavi for his kind help.

Funding

This work was financially supported by a research grant from Shiraz University, Shiraz, Iran (Grant No. 96-GR-VT-1).

Author information

Authors and Affiliations

  1. Department of Pathobiology, School of Veterinary Medicine, Shiraz University, P.O. Box 71441-69155, Shiraz, Iran

    Ehsan Rakhshandehroo, Seyed Mostafa Razavi, Razieh Farzaneh, Atefeh Esmailnejad & Mohammad Asadpour

  2. Department of Clinical Studies, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

    Shahab Shams

Authors
  1. Ehsan Rakhshandehroo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Seyed Mostafa Razavi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Razieh Farzaneh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Atefeh Esmailnejad
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Mohammad Asadpour
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shahab Shams
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

ER and SMR designed and supervised the experiment. RF carried out the research technically; AE and MA had roles in analyzing data and writing the manuscript. SS participated in collecting the samples and conducting the study.

Corresponding author

Correspondence to Ehsan Rakhshandehroo.

Ethics declarations

Conflict of interest

All authors declares that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rakhshandehroo, E., Razavi, S.M., Farzaneh, R. et al. Phylogenetic analysis of goat warble fly (Przhevalskiana silenus) based on mitochondrial COI gene. J Parasit Dis 43, 304–307 (2019). https://doi.org/10.1007/s12639-019-01093-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12639-019-01093-8

Keywords

Search

Navigation