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Anthelmintic efficacy of Moringa oleifera seed methanolic extract against Fasciola hepatica

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Abstract

The current study was carried out to assess in vitro and in vivo effects of Moringa oleifera seed methanolic extract on Fasciola hepatica to develop an alternative source of treatment. The in vitro ovicidal effect of M. oleifera seed extract on immature F. hepatica eggs has provided evidence of inhibitory activity on the vitality and hatchability of F. hepatica eggs. This inhibitory activity was concentration-dependent and also correlated strongly with the exposure time. In the in vivo trial, the oral administration of F. hepatica experimentally infected rabbits with doses of 150 mg/kg BW prepared extract per day for 3 consecutive days on the 63rd day post infection confirmed potent fasciolicide activity of the extract. A gradual decrease in fecal egg count (FEC) was detected from the 1st day post treatment until reaching 100% FEC reduction by the 7th day post treatment. No flukes could be found at post mortem examinations. Significant increments of serum total protein, globulin, the activities of ALT and AST, total cholesterol, triglycerides and urea were recorded during the period of infection, which were improved by treatment. Remarkable histopathological alterations were observed in the infected liver and gallbladder tissues which decreased clearly in the treated rabbits. This study proposes that the used extract has promising and potent fasciolicide activity.

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Acknowledgements

This work was funded from a co-project between the Academy of Scientific Research in Egypt and the Bulgarian Academy of Science, entitled “Recent studies of pathogenetic mechanism of neoplastic fasciolosis and advanced control methods.”

Authors’ contributions

OMK designed, supervised and directed the experiment. VN, NT, and MG carried out the experimental infection to provide metacercaria. OMK, NMFH, DS, EBA, and HAS conducted the in vitro and in vivo experiments and the laboratory work of the samples. SA performed the biochemical and histopathological studies. OMK, NMF, DS, EBA, SNA and HAS analyzed and discussed the resultant data. OMK, NMFH and HAS implemented writing the manuscript. OMK and NMFH revised and reviewed the manuscript for publication. All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Department of Parasitology and Animal Diseases, National Research Centre (NRC), P.O. Box 12622, 133 El-Behouth Street, Dokki, Cairo, Egypt

    Omnia M. Kandil, Noha M. F. Hassan, Doaa Sedky, Emad B. Ata, Somia A. Nassar & Hatem A. Shalaby

  2. Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev St, Bl. 25, 1113, Sofia, Bulgaria

    Veselin Nanev, Neli Tsocheva-Gaytandzhieva & Margarita Gabrashanska

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  1. Omnia M. Kandil
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  2. Noha M. F. Hassan
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Correspondence to Omnia M. Kandil.

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Kandil, O.M., Hassan, N.M.F., Sedky, D. et al. Anthelmintic efficacy of Moringa oleifera seed methanolic extract against Fasciola hepatica. J Parasit Dis 42, 391–401 (2018). https://doi.org/10.1007/s12639-018-1014-y

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  • DOI: https://doi.org/10.1007/s12639-018-1014-y

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