Skip to main content
Log in

Diagnosis of bovine sarcocystosis by immunoflourescent antibody technique

  • Original Article
  • Published:
Journal of Parasitic Diseases Aims and scope Submit manuscript

Abstract

Studies on sarcocystosis showed an overall sero-prevalence of 79.46% by IFAT which included 80.14% in cattle and 78.59% in buffaloes out of 716 animals screened. The antibody titers of six randomly selected positive samples from different age groups of <2, 2–5, 5–10 and >10 years old bovines ranged from 16 to 64, 32 to 256, 32 to 128 and 16 to 64 with an average titer of 32 ± 2.92, 106.6 ± 34, 74.6 ± 17 and 34.6 ± 9, respectively. Significantly, lower rates of infection were observed in the cattle of below 2 years (60%) age and an ascending rate of infection in the age groups of 2–5 years (81.33%), 5–10 years (80.52%) and above 10 years (90.9%).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Abdel Rahman SM (2001) Serodiagnosis of two zoonotic parasites (Toxoplasma & Sarcocystis) in cattle. In: First Congress of Food Hygiene & Human Health, Faculty of Veterinary Medicine, Assiut, Egypt

  • Barrows PL, Prestwood AK, Green CE (1982) Experimental Sarcocystis suicanis infections: disease in growing pigs. Am J Vet Res 43:1409–1412

    CAS  PubMed  Google Scholar 

  • Bottner A, Chalerston WAG, Pomroy WE, Rommel M (1987) The prevalence and identity of Sarcocystis in beef of cattle in New Zeland. Vet Parasitol 24(3–4):157–168

    Article  CAS  PubMed  Google Scholar 

  • Cerna Z, Merhautova V (1981) Sarcocystis in cattle and sheep at Prague abattoir. Folia Parasitol 28:125–129

    CAS  PubMed  Google Scholar 

  • Claveria FG, Petersen B, Macabagdal MR, Farolan RJ, Farrol MA, Gonzalvo F, Cadiz R, Ajero R, Roque R, Lozano G (1997) A survey of bovine, bubaline and swine sarcocystosis in the Philippines. Southeast Asian J Trop Med Public Health 28(1):173–178

    PubMed  Google Scholar 

  • Collier L, Balows A, Sussman M (1998) Sarcocystis, Isospora and cyclospora. Topley and Welson’s micobiology and microbial infections, vol 5, 9th edn. Oxford University Press, Inc, New York, pp 319–326

    Google Scholar 

  • Dubey JP (1976) A review of Sarcocystis of domestic animals and of other Coccidia of cats and dogs. J Am Vet Med Assoc 169(10):1061–1078

    CAS  PubMed  Google Scholar 

  • El-Dakhly KM, Khaled A, El-Nesr MS, El-Nahass ES, Hirata A, Sakai H, Yanai T (2011) Prevalence and distribution patterns of Sarcocystis spp. in buffaloes in Beni-Suef, Egypt. Trop Anim Health Prod 43:1549–1554

    Article  PubMed  Google Scholar 

  • El-Nazer M, Abdil-Azim AH (2000) Seropositivity to Sarcocystis antigen in attendants of rheumatology clinic in Sohag University Hospital. South Val Med 4(2):145–155

    Google Scholar 

  • Habeeb YS, Selim MA, Ali MSM, Mahmoud LA, Abdel-Hady AM, Shfei A (1996) Serological diagnosis of extra-intestinal sarcocystosis. J Egypt Soc Parasitol 26(2):393–400

    CAS  PubMed  Google Scholar 

  • Hettiarachchi DC, Rajapakse RPVJ (2008) Antigenic analysis of bovine Sarcocystis spp in Sri Lanka. J Nat Sci Found Sri Lanka 36(3):239–244

    CAS  Google Scholar 

  • Huong LTT (1999) Prevalence of Sarcocystis spp. in water buffaloes in Vietnam. Vet Parasitol 86(1):33–39

    Article  CAS  PubMed  Google Scholar 

  • Levine ND (1973) Sarcocystis, Toxoplasma and related protozoa. In: Protozoan parasites of Animals and Man, 2nd edn. Burgess Minneapolis, pp 292-294

  • Li QQ, Yang ZQ, Zuo YX, Attwood SW, Chen XW, Zhang YP (2002) A PCR-based RFLP analysis of Sarcocystis cruzi (protozoa: sarcocystidae) in Yunnan. Province, PR China, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host. J Parasitol 88(6):1259–1261

    Article  CAS  PubMed  Google Scholar 

  • Lunde MN (1973) Laboratory methods in the diagnosis of Toxoplasmosis. Health Lab Sci 10:328

    Google Scholar 

  • Nevole M (1982) Prevalence of sarcocystosis in our cattle. Veterinafstvi 32:24

    Google Scholar 

  • Pana HF, Ogessawara S, Sinhorini IL (2001) Occurrence of cattle sarcocystosis species in raw kibbe from Arabian food establishments in the city of Sao Paulo, Brazil, and experimental transmission to humans. J Parasitol 87:1459–1465

    Google Scholar 

  • Pappas MG, Lunde MN, Hajkowski R, McMahon J (1986) Determination of IgM and IgG antibodies to Toxoplasma using the IFA test, ELISA, and Dot-ELISA procedures. Vet Parasitol 20(1–3):31–42

    Article  CAS  PubMed  Google Scholar 

  • Renterghem LV, Nimmen LV (1976) Indirect immunofluorescence in toxoplasmosis: frequency, nature and specificity of polar staining. Zbl Bakt Hyg I Abt Orig 235:559–565

    Google Scholar 

  • Sayed FG, Shaheen MSI, Arafa MI, Koraa HM (2008) Sarcocystis infection in cattle at assiut abattoir: microscopical and serological. Assiut Univ Bull Environ Res 11:47–56

    Google Scholar 

  • Tadros W, Hazeohoff W, Laarmanm JJ (1980) Cross reaction in the indirect fluorescent antibodies among six species of Sarcocystis. Trop Geogr Med 6:150–153

    Google Scholar 

  • Tenter AM (1995) Current research on Sarcocystis spp of domestic animals. Int J Paratsitol 25:1311–1330

    Article  CAS  Google Scholar 

  • Toole D (1987) Experimental ovine sarcocystosis: sequential ultrastructural pathology in skeletal muscle. J Comp Path 97:51–60

    Article  Google Scholar 

  • USDHEW (1976) Manual—a procedural guide for the performance of the serology of toxoplasmosis. Centre for Disease Control Atlanta, (US Department of Health, Education and Welfare), Atlanta

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Dasmabai Banothu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Banothu, D., Manchukonda, U. & Rayulu, V.C. Diagnosis of bovine sarcocystosis by immunoflourescent antibody technique. J Parasit Dis 41, 795–798 (2017). https://doi.org/10.1007/s12639-017-0890-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12639-017-0890-x

Keywords

Navigation