Abstract
Studies on sarcocystosis showed an overall sero-prevalence of 79.46% by IFAT which included 80.14% in cattle and 78.59% in buffaloes out of 716 animals screened. The antibody titers of six randomly selected positive samples from different age groups of <2, 2–5, 5–10 and >10 years old bovines ranged from 16 to 64, 32 to 256, 32 to 128 and 16 to 64 with an average titer of 32 ± 2.92, 106.6 ± 34, 74.6 ± 17 and 34.6 ± 9, respectively. Significantly, lower rates of infection were observed in the cattle of below 2 years (60%) age and an ascending rate of infection in the age groups of 2–5 years (81.33%), 5–10 years (80.52%) and above 10 years (90.9%).
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References
Abdel Rahman SM (2001) Serodiagnosis of two zoonotic parasites (Toxoplasma & Sarcocystis) in cattle. In: First Congress of Food Hygiene & Human Health, Faculty of Veterinary Medicine, Assiut, Egypt
Barrows PL, Prestwood AK, Green CE (1982) Experimental Sarcocystis suicanis infections: disease in growing pigs. Am J Vet Res 43:1409–1412
Bottner A, Chalerston WAG, Pomroy WE, Rommel M (1987) The prevalence and identity of Sarcocystis in beef of cattle in New Zeland. Vet Parasitol 24(3–4):157–168
Cerna Z, Merhautova V (1981) Sarcocystis in cattle and sheep at Prague abattoir. Folia Parasitol 28:125–129
Claveria FG, Petersen B, Macabagdal MR, Farolan RJ, Farrol MA, Gonzalvo F, Cadiz R, Ajero R, Roque R, Lozano G (1997) A survey of bovine, bubaline and swine sarcocystosis in the Philippines. Southeast Asian J Trop Med Public Health 28(1):173–178
Collier L, Balows A, Sussman M (1998) Sarcocystis, Isospora and cyclospora. Topley and Welson’s micobiology and microbial infections, vol 5, 9th edn. Oxford University Press, Inc, New York, pp 319–326
Dubey JP (1976) A review of Sarcocystis of domestic animals and of other Coccidia of cats and dogs. J Am Vet Med Assoc 169(10):1061–1078
El-Dakhly KM, Khaled A, El-Nesr MS, El-Nahass ES, Hirata A, Sakai H, Yanai T (2011) Prevalence and distribution patterns of Sarcocystis spp. in buffaloes in Beni-Suef, Egypt. Trop Anim Health Prod 43:1549–1554
El-Nazer M, Abdil-Azim AH (2000) Seropositivity to Sarcocystis antigen in attendants of rheumatology clinic in Sohag University Hospital. South Val Med 4(2):145–155
Habeeb YS, Selim MA, Ali MSM, Mahmoud LA, Abdel-Hady AM, Shfei A (1996) Serological diagnosis of extra-intestinal sarcocystosis. J Egypt Soc Parasitol 26(2):393–400
Hettiarachchi DC, Rajapakse RPVJ (2008) Antigenic analysis of bovine Sarcocystis spp in Sri Lanka. J Nat Sci Found Sri Lanka 36(3):239–244
Huong LTT (1999) Prevalence of Sarcocystis spp. in water buffaloes in Vietnam. Vet Parasitol 86(1):33–39
Levine ND (1973) Sarcocystis, Toxoplasma and related protozoa. In: Protozoan parasites of Animals and Man, 2nd edn. Burgess Minneapolis, pp 292-294
Li QQ, Yang ZQ, Zuo YX, Attwood SW, Chen XW, Zhang YP (2002) A PCR-based RFLP analysis of Sarcocystis cruzi (protozoa: sarcocystidae) in Yunnan. Province, PR China, reveals the water buffalo (Bubalus bubalis) as a natural intermediate host. J Parasitol 88(6):1259–1261
Lunde MN (1973) Laboratory methods in the diagnosis of Toxoplasmosis. Health Lab Sci 10:328
Nevole M (1982) Prevalence of sarcocystosis in our cattle. Veterinafstvi 32:24
Pana HF, Ogessawara S, Sinhorini IL (2001) Occurrence of cattle sarcocystosis species in raw kibbe from Arabian food establishments in the city of Sao Paulo, Brazil, and experimental transmission to humans. J Parasitol 87:1459–1465
Pappas MG, Lunde MN, Hajkowski R, McMahon J (1986) Determination of IgM and IgG antibodies to Toxoplasma using the IFA test, ELISA, and Dot-ELISA procedures. Vet Parasitol 20(1–3):31–42
Renterghem LV, Nimmen LV (1976) Indirect immunofluorescence in toxoplasmosis: frequency, nature and specificity of polar staining. Zbl Bakt Hyg I Abt Orig 235:559–565
Sayed FG, Shaheen MSI, Arafa MI, Koraa HM (2008) Sarcocystis infection in cattle at assiut abattoir: microscopical and serological. Assiut Univ Bull Environ Res 11:47–56
Tadros W, Hazeohoff W, Laarmanm JJ (1980) Cross reaction in the indirect fluorescent antibodies among six species of Sarcocystis. Trop Geogr Med 6:150–153
Tenter AM (1995) Current research on Sarcocystis spp of domestic animals. Int J Paratsitol 25:1311–1330
Toole D (1987) Experimental ovine sarcocystosis: sequential ultrastructural pathology in skeletal muscle. J Comp Path 97:51–60
USDHEW (1976) Manual—a procedural guide for the performance of the serology of toxoplasmosis. Centre for Disease Control Atlanta, (US Department of Health, Education and Welfare), Atlanta
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Banothu, D., Manchukonda, U. & Rayulu, V.C. Diagnosis of bovine sarcocystosis by immunoflourescent antibody technique. J Parasit Dis 41, 795–798 (2017). https://doi.org/10.1007/s12639-017-0890-x
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DOI: https://doi.org/10.1007/s12639-017-0890-x