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Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt

Journal of Parasitic Diseases volume 40, pages 901–905 (2016)Cite this article

Abstract

Cryptosporidium is a significant cause of diarrhea worldwide especially in children. Infection may end fatally in immunocompromised patients. Multi-attribute analysis was used to determine the lab utility of 4 diagnostics; coproscopy of AF stained fecal smear, fecal immunoassays by ICT and ELISA and copro-nPCR assay targeting Hsp90 gene, for detection of Cryptosporidium in stool of 250 Egyptian children (150 diarrheic and 100 non-diarrhaeic children). Also, to determine Cryptosporidium molecular prevalence. Cryptosporidium was an important enteric pathogen among both diarrheic and non-diarrheic study children with a clearly high prevalence of 16.4 % (n = 41). Conventional methods had perfect specificity (100 %) but couldn`t be used as a consistent single detection method due to their lowered sensitivities. Multi-attribute analysis ranked nPCR the highest test for lab use. Being the test with the best diagnostic yield, nPCR is a reliable diagnostic test and is going to replace conventional methods for reliable detection of Cryptosporidium.

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The authors declare that they have no Conflict of interests.

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Authors and Affiliations

  1. Medical Parasitology, Kasr Al-Ainy School of Medicine, Cairo University, Cairo, Egypt

    Marwa M. I. Ghallab, Inas Z. Abdel Aziz, Eman Y. Shoeib & Ayman A. El-Badry

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  1. Marwa M. I. Ghallab
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  2. Inas Z. Abdel Aziz
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  3. Eman Y. Shoeib
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  4. Ayman A. El-Badry
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Correspondence to Marwa M. I. Ghallab.

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Ghallab, M.M.I., Aziz, I.Z.A., Shoeib, E.Y. et al. Laboratory utility of coproscopy, copro immunoassays and copro nPCR assay targeting Hsp90 gene for detection of Cryptosporidium in children, Cairo, Egypt. J Parasit Dis 40, 901–905 (2016). https://doi.org/10.1007/s12639-014-0601-9

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  • DOI: https://doi.org/10.1007/s12639-014-0601-9

Keywords

  • Cryptosporidium
  • Hsp90
  • Copro-DNA
  • Nested PCR
  • Multi-attribute
  • Fecal immunoassay
  • ELISA
  • ICT
  • AF stain