Prevalence and molecular epidemiology of Cryptosporidium parvum in dairy calves in Punjab (India)
- 157 Downloads
Cryptosporidium parvum is an important zoonotic protozoan parasite that infects the gastrointestinal tract of vertebrate animals and man. The current study was contemplated for molecular detection of Cryptosporidium species prevalent in dairy calves in Punjab, India. A total of 302 faecal samples were screened by modified Ziehl-Neelsen staining technique for the detection of Cryptosporidium oocysts. Molecular characterisation was done using PCR followed by sequence analysis of the representative isolates. An overall prevalence of 26.15 % was obtained with the highest prevalence obtained in 0–30 day old calves in both diarrhoeic and non-diarrhoeic animals. PCR analysis revealed the expected bands at 1,325 and 835 bp from all the isolates for primary and secondary/nested PCR respectively. Ten representative samples were sequenced in both directions. Phylogenetic analysis revealed the presence of C. parvum in all the samples. The high rate of calves infected with C. parvum can act as a great source of zoonotic cryptosporidiosis which indicates a potential risk of zoonotic transmission from animal to human beings in Punjab (India).
KeywordsPrevalence Dairy calves Cryptosporidium parvum Punjab India
Conflict of interest
No financial or personal relationships between the authors and other people or organizations have inappropriately influenced (bias) this work.
- Dubey JP, Fayer R, Rao JR (1992) Cryptosporidial oocysts in faeces of water buffalo and zebu calves in India. J Vet Parasitol 6:55–56Google Scholar
- Jeyaba L, Ray DD (2005) Cryptosporidial infection in cattle and buffaloes. J Vet Parasitol 19:165–166Google Scholar
- Kumar D, Sreekrishnan R, Das SS (2004) Cryptosporidiosis in man and animals in Pondicherry. Ind J Anim Sci 74:261–263Google Scholar
- Nooruddin M, Sarma DK (1987) Role of Cryptosporidium in calf diarrhoea: in annual report. Livestock Advisor 12:49Google Scholar
- O’Handley RM, Cockwill C, McAllister TA, Jelinski M, Morck DW, Olson ME (1999) Duration of naturally acquired giardiosis and cryptosporidiosis in dairy calves and their association with diarrhoea. J Amer Vet Med Assoc 214:391–396Google Scholar
- OIE (2008) Cryptosporidiosis terrestrial manual chapter 2.9.4: 1192–15Google Scholar
- Shobhamani B, Singari NA, Syamasundar N (2005) A study on clinical manifestations of cryptosporidiosis in calves. Ind Vet J 83:677–678Google Scholar