Evaluation of the antileishmanial and cytotoxic effects of various extracts of garlic (Allium sativum) on Leishmania tropica
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Cutaneous leishmaniasis (CL) is a major public health problem in tropical and subtropical countries worldwide. Treatment of CL by pentavalent antimony compounds remains a challenge because of limited efficacy, toxic side effects and drug resistance. In the present study, in vitro antileishmanial and cytotoxic activity of garlic extracts against promastigote forms of Leishmania tropica and murine macrophages was evaluated by colorimetric cell viability (MTT) assay. The results revealed that the methanolic and aqueous extracts of garlic were effective in inhibiting promastigote growth of L. tropica with IC50 (50 % inhibitory concentrations) values 12.3 and 19.2 µg/ml, respectively. In addition, methanolic and aqueous extracts of garlic showed low cytotoxicity against murine macrophages with CC50 (cytotoxicity concentration for 50 % of cells) values 291.4 and 348.2 µg/ml, respectively. Findings of present study were the first step in the search for new antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in volunteer human subjects.
KeywordsPromastigote Murine macrophage MTT Cutaneous leishmaniasis Cytotoxicity
We would like to thank Ms. Razieh Tavakil for cultivation of promastigotes of L. tropica.
Conflict of interest
The authors declare that there is no conflict of interest in this study.
- Behnia M, Haghighi A, Komeilizadeh H, Tabaei SJ, Abadi A (2008) In vitro antiamoebic activity of Iranian Allium sativum in comparison with metronidazole against Entamoeba histolytica. Iran J Parasitol 3(4):32–38Google Scholar
- Cai Y (1991) Anticryptococcal and antiviral properties of garlic. Cardiol Pract 9:11Google Scholar
- Mahmoudvand H, Sharifi I, Harandi MF, Shokohi M, Shakibaie M, Riabi TR et al (2014a) Anti-leishmania effects of methotrexate (MTX) alone and incombination with meglumine antimoniate (MA) against Iranian isolate of sensitive and MA-resistant Leishmania tropica: an in vitro assay. Asian Pacific J Trop Med 4:412–420Google Scholar
- Mahmoudvand H, Sharififar F, Sharifi I, Ezatpour B, Harandi MF, Makki, Jahanbakhsh S (2014b) In Vitro inhibitory effect of Berberis vulgaris (Berberidaceae) and its main component, berberine against different Leishmania species. Iran J Parasitol 9(1):28–36Google Scholar
- Meng Y, Lu D, Guo N, Reed E, Zhou GZ, Zhang LB (1993) Anti-HCMV effect of garlic components. Virol Sin 8:147–150Google Scholar
- Moazeni M, Nazer A (2010) In vitro Effectiveness of Garlic (Allium sativum) Extract on Scolices of Hydatid Cyst. World J Surg 34:2677–2681Google Scholar
- Wabwoba B, Anjili CO, Ngeiywa M, Ngure PK, Kigondu EM, Ingonga J, Makwail J (2010) Experimental chemotherapy with Allium sativum (Liliaceae) methanolic extract in rodents infected with Leishmania major and Leishmania donovani. J Vector Borne Dis 47:160–167Google Scholar
- World Health Organization (2010) Control of the leishmaniasis. WHO (Technical Report Series 949) Geneva, p 5–12Google Scholar