Journal of Parasitic Diseases

, Volume 40, Issue 2, pp 423–426 | Cite as

Evaluation of the antileishmanial and cytotoxic effects of various extracts of garlic (Allium sativum) on Leishmania tropica

  • Hossein Mahmoudvand
  • Peyman Sepahvand
  • Sareh Jahanbakhsh
  • Mozhgan Azadpour
Original Article


Cutaneous leishmaniasis (CL) is a major public health problem in tropical and subtropical countries worldwide. Treatment of CL by pentavalent antimony compounds remains a challenge because of limited efficacy, toxic side effects and drug resistance. In the present study, in vitro antileishmanial and cytotoxic activity of garlic extracts against promastigote forms of Leishmania tropica and murine macrophages was evaluated by colorimetric cell viability (MTT) assay. The results revealed that the methanolic and aqueous extracts of garlic were effective in inhibiting promastigote growth of L. tropica with IC50 (50 % inhibitory concentrations) values 12.3 and 19.2 µg/ml, respectively. In addition, methanolic and aqueous extracts of garlic showed low cytotoxicity against murine macrophages with CC50 (cytotoxicity concentration for 50 % of cells) values 291.4 and 348.2 µg/ml, respectively. Findings of present study were the first step in the search for new antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in volunteer human subjects.


Promastigote Murine macrophage MTT Cutaneous leishmaniasis Cytotoxicity 



We would like to thank Ms. Razieh Tavakil for cultivation of promastigotes of L. tropica.

Conflict of interest

The authors declare that there is no conflict of interest in this study.


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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Hossein Mahmoudvand
    • 1
  • Peyman Sepahvand
    • 2
  • Sareh Jahanbakhsh
    • 1
  • Mozhgan Azadpour
    • 3
  1. 1.Leishmaniasis Research CenterKerman University of Medical SciencesKermanIran
  2. 2.Department of Medical VirologyAhvaz University of Medical SciencesAhvazIran
  3. 3.Razi Herbal Medicines Research CenterLorestan University of Medical SciencesKhorramabadIran

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