Journal of Parasitic Diseases

, Volume 40, Issue 2, pp 330–338 | Cite as

Utilizing ribosomal DNA gene marker regions to characterize the metacercariae (Trematoda: Digenea) parasitizing piscine intermediate hosts in Manipur, Northeast India

  • Voleentina D. Athokpam
  • Donald B. Jyrwa
  • Veena Tandon
Original Article


Freshwater fishes in Manipur, Northeast India frequently harbour several types of metacercariae, which based on morphological criteria were identified as Clinostomoides brieni, Euclinostomum heterostomum (Clinostomidae) and Polylekithum sp. (Allocreadiidae). Molecular techniques utilizing PCR amplification of rDNA regions of larger subunit (LSU or 28S), smaller subunit (SSU or 18S) and inter transcribed spacers (ITS1, 2) were used for molecular characterization of these types. Sequences generated from the metacercariae were compared with their related sequences available in public databases; an analysis of the identity matrices and phylogenetic trees constructed was also carried out, which confirmed their identification. Similarly, the sequences generated from Polylekithum sp. were found to be highly similar to the species of the same genus. The rDNA ITS2 secondary structure provided additional confirmation of the robustness of the molecular marker as a tool for taxon-specific characterization.


Molecular rDNA ITS2 28S 18S Metacercaria Fish hosts Clinostomidae Allocreadiidae India 



VDA acknowledges the award of Research Fellowship for meritorious students by University Grants Commission (UGC) and Senior Research Fellowship by Council of Scientific & Industrial Research (CSIR), Government of India.


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Copyright information

© Indian Society for Parasitology 2014

Authors and Affiliations

  • Voleentina D. Athokpam
    • 1
  • Donald B. Jyrwa
    • 1
  • Veena Tandon
    • 1
  1. 1.Department of ZoologyNorth-Eastern Hill UniversityShillongIndia

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