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Histopathological and morphological studies on natural Cysticercus fasciolaris infection in liver of Wistar rats

Abstract

Descriptive morphology of multifocal hepatic cysts found in eight of forty five (17.78 %) Wistar rats sacrificed during pharmacological studies related to herbal formulations was studied. The creamish to white cysts were of varying sizes, ranging from 3–8 mm in diameter. Morphological studies of these cysts depicted the presence of metacestodes of Taenia taeniaeformis i.e. Cysticercus fasciolaris inside them. The scolex of metacestode revealed four suckers and rostellum armed with two distinct rows of characteristic pen knife shaped hooks (characteristics of taeniid cestodes). The average size of large hooks was 392.92 ± 10.12 µ and that of small hooks was 240.64 ± 14.26 µ. The average size of suckers was 304.36 ± 12.33 µ. Histopathology of hepatic tissue surrounding the cysts revealed zones of fatty change, inflammation, granulation tissue and metaplasia. However, the histopathology of stomach and small intestines didn’t show any significant lesions.

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References

  • Al- Jashamy K, El Salihi K, Sheikh A, Saied H (2004) Cysticercosis in rat infected with C. fasciolaris. In: Proceedings of 9th National conference on medical sciences. University Sciences Malaysia, Kubang Kerian, pp 185

  • Al-Jashamy K, Islam MN (2007) Morphological study of Taenia taeniaeformis scolex under scanning electron microscopy using hexamethyldislazane. Ann Microbiol 7:80–83

    Google Scholar 

  • Al-Najjar SS, Kadhimand FS, Abdalrziak NA (2009) Parasitological and Pathological study of the Cysticercus fasciolaris naturally infested white mice. Al Anbar J Vet Sci 2:43–47

    Google Scholar 

  • Armando R, Irizarry-Rovira Alexander W, Matthew B (2007) Taenia taeniaeformis induced metastatic hepatic sarcoma in a pet rat (Rattus norvegicus). J Exot Pet Med 16:45–48

    Article  Google Scholar 

  • Dunning WF, Curtis MR (1946) Multiple peritoneal sarcoma in rats from intraperitoneal injection of washed, ground Taenia larvae. Cane Res 6:668–670

    CAS  Google Scholar 

  • Ekanayake S, Warnasuriya ND, Samarakoon PS, Abewickrama H, Kuruppuarachchi ND, Dissanaike AS (1999) An unusual ‘infection’ of a child in Sri Lanka with Taenia taeniaeformis of the cat. Ann Trop Med Parasitol 93:869–873

    CAS  Article  PubMed  Google Scholar 

  • Hanes MA (1995) Fibro sarcomas in two rats arising from hepatic cysts of Cysticercus fasciolaris. Vet Pathol 32:441–444

    CAS  Article  PubMed  Google Scholar 

  • Hsu CK (1979) The laboratory rat. In: Baker HG, Lindsey JR, Weisbroth SH (eds) Biology and disease. Academic Press, New York, pp 314–315

    Google Scholar 

  • Kass PH, Barnes WG, Spangler WL, Chomel BB, Culbertson MR (1993) Epidemiologic evidence for a causal relation between vaccination and fibrosarcoma tumorigenesis in cats. Am Vet Med Assoc 203:396–405

    CAS  Google Scholar 

  • Kumar JM, Reddy PL, Aparna V, Srinivas G, Nagarajan P, Venkatesan R, Sreekumar C, Sesikaran B (2006) Strobilocercus fasciolaris infection with hepatic sarcoma and gastroenteropathy in a Wistar colony. J Vet Parasitol 141:362–367

    Article  Google Scholar 

  • Luna HT, Lee G (1968) Manual of histological staining methods of the armed forces. Institute of pathology. 3rd ed. Plackiston Division McGraw Hill Book co. New York Toronto, London and Sydney

  • McCoy GW (1909) A preliminary report of tumors found in wild rats. J Med Res 21:283

    Google Scholar 

  • Meyer CM, Oslen WO (1975) Essentials of parasitology. W. M. C Brown Company Publishers, Dubuque

    Google Scholar 

  • Miyazaki I (1991) Helminthic zoonoses. International Medical Foundation of Japan, Tokyo, p 494

    Google Scholar 

  • Singla LD, Singla N, Prasad VR, Sandhu BS, Singh J (2003) Occurrence and pathomorphological observations of Cysticercus fasciolaris in lesser bandicoot rats in India. In: Proceedings of Rats, Mice and People: Rodent Biology and Management. Australian Centre for International Agricultural Research, Canberra, pp 43–46

  • Singla LD, Singla N, Parshad VR, Juyal PD, Sood NK (2008) Rodents as reservoirs of parasites in India. Integr Zool 3:21–26

    Article  PubMed  Google Scholar 

  • Singla LD, Aulakh GS, Sharma R, Juyal PD, Singh J (2009) Concurrent infection of Taenia taeniaeformis and Isospora felis in a stray kitten: a case report. Vet Med 54(2):81–83

    Google Scholar 

  • Singla N, Singla LD, Gupta K, Sood NK (2013) Pathological alterations in natural cases of Capillaria hepatica infection alone and in concurrence with Cysticercus fasciolaris in Bandicota bengalensis. J Parasit Dis 37(1):16–20

    PubMed  Google Scholar 

  • Soulsby EJL (1982) Helminths, arthropods and protozoa of domesticated animals. Baillere Tindall, London

    Google Scholar 

Download references

Acknowledgments

Authors are highly obliged to Dr. V.K. Dumka, Professor, Department of Veterinary Pharmacology & Toxicology as well as to Professor- cum- Head, Department of Veterinary Pharmacology and Toxicology for sparing the specimens from the sacrificed animals for the present study.

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Correspondence to Lachhman Das Singla.

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Moudgil, A.D., Singla, L.D., Gupta, K. et al. Histopathological and morphological studies on natural Cysticercus fasciolaris infection in liver of Wistar rats. J Parasit Dis 40, 255–258 (2016). https://doi.org/10.1007/s12639-014-0488-5

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  • DOI: https://doi.org/10.1007/s12639-014-0488-5

Keywords

  • Cysticercus fasciolaris
  • Hepatic cysts
  • Histopathology
  • Metacestode
  • Wistar rats