Abstract
Trichomoniasis, the disease caused by the flagellate protozoan Trichomonas vaginalis is the sexually transmitted infection with the largest annual incidence. Metronidazole is the drug of choice recommended for the treatment of human trichomoniasis but it can lead to drug resistance and many other adverse effects. So, it is necessary for new alternatives for the treatment of this infection. Medicinal plants or herbs could be good alternative regimens to be inexpensive, effective and safe to use. In the present study, the therapeutic potential of Nigella sativa aqueous and alcoholic extracts as well as seeds oil was examined. Different concentrations of these plant preparations were incubated in vitro with cultivated T. vaginalis trophozoites and its effect on growth was compared with metronidazole under the same conditions. Both the alcoholic extract and oil proved to be valuable agents as efficient as metronidazole in treating T. vaginalis infection. The remarkable effect of N. sativa oil may be attributed to the fact that the active principles extracted from N. sativa seeds are mostly from its essential oil (omega 3, 6, 9 as well as 7 fatty acids). However, further experimental and clinical investigations are needed to evaluate and standardize the doses of these natural products to be safe and efficient.
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Abdel-Zaher AO, Abdel-Rahman MS, Elwasei FM (2011) Protective effect of Nigella sativa oil against tramadol-induced tolerance and dependence in mice: role of nitric oxide and oxidative stress. Neurotoxicology 32:725–733
Abel-Salam BK (2012) Immunomodulatory effects of black seeds and garlic on alloxan-induced diabetes in albino rat. Allergol Immunopathol (Madr) 40:336–340
Abu El Ezz NM (2005) Effect of Nigella sativa and Allium cepa oils on Trichinellaspiralis in experimentally infected rats. J Egypt Soc Parasitol 35:511–523
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi A, Siddique NA, Damanhouri ZA, Anwar F (2013) A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pac J Trop Biomed 3:337–352
Al-Heali FM, Rahemo Z (2006) The combined effect of two aqueous extracts on the growth of Trichomonas vaginalis, in vitro. Turkiye Parazitol Derg 30(4):272–274
Al-Homidan A, Al-Qarawi AA, Al-Waily SA, Adam SE (2002) Response of broiler chicks to dietary Rhazyastricta and Nigella sativa. Br Poult Sci 43:291–296
Alzanbagi NA, Salem HS, Al Braiken F (2005) Trichomoniasis among women with vaginal discharge in Jeddah city, Saudi Arabia. J Egypt Soc Parasitol 35:1071–1080
Assayed ME (2010) Radioprotective effects of black seed (Nigella sativa) oil against hemopoietic damage and immunosuppression in gamma-irradiated rats. Immuno pharmacol Immuno toxicol 32:284–296
Badary OA, Al-Shabanah OA, Nagi MN, Al-Bekairi AM, Elmazar MA (1998) Acute and subchronic toxicity of thymoquinone in mice. Drug Dev Res 44:56–61
Bishara SA, Masoud SI (1992) Effect of Nigella sativa extract on experimental giardiasis. New Egypt J Med 7:1–3
Borchardt KA, Zhang MZ, Shing H, Flink KA (1997) Comparison of the sensitivity of the In-Pouch TV, Diamond’s and Trichosel media for detection of Trichomonas vaginalis. Genitourin Med 73(4):297–298
Boskabady MH, Mohsenpoor N, Takaloo L (2010) Antiasthmatic effect of Nigella sativa in airways of asthmatic patients. Phytomedicine 17:707–713
Boyaval P, Corre C, Dupuis C, Roussel E (1995) Effects of free fatty acids on propionic acid bacteria. Lait 75:17–29
Brandelli CL, Vieira P, de B, Macedo AJ, Tasca T (2013) Remarkable anti-Trichomonas vaginalis activity of plants traditionally used by the Mbyá-Guarani Indigenous group in Brazil. BioMed Res Int 2013(2013):1–7
Cates W Jr (1999) Estimates of the incidence and prevalence of sexually transmitted diseases in the United States. American Social Health Association Panel. Sex Transm Dis 26:2–7
Cheesbrough M (1998) Parasitological tests In: Cheesbrough M (ed) District laboratory practice in tropical countries Part 1, Cambridge Low Price Edn, Cambridge University Press, Cambridge, p 178–310
Cotch MF, Pastorek JG, Nugent RP, Hillier SL, Gibbs RS, Martin DH, Eschenbach DA, Edelman R, Carey JC, Regan JA, Krohn MA, Klebanoff MA, Rao AV, Rhoads GG (1997) Trichomonas vaginalis associated with low birth weight and preterm delivery. The vaginal infections and prematurity study group. Sex Trans Dis 24(6):353–360
Davies DG, Marques CNH (2009) A fatty acid messenger is responsible for inducing dispersion in microbial biofilms. J Bacteriol 191:1393–1403
Diamond LS (1957) The establishment of various trichomonads of animals and man in axenic cultures. J Parasitol 43:488–490
El Daly ES (1998) Protective effect of cysteine and vitamin E, Crocus sativus and Nigella sativa extracts on cisplatin-induced toxicity in rats. J Pharm (Belg) 53:87–93
El Wakil HS (2007) Evaluation of in vitro effect of Nigella sativa aqueous extract on Blastocystis hominis isolates. J Egypt Soc Parasitol 37(3):801–813
El-Sherbiny GM, El Sherbiny ET (2011) The effect of commiphoramol (Myrrh) in treatment of Trichomonas vaginalis infection. Iran Red Crescent Med J 13(7):480–486
Fernando C, Lilian Y, Amparo T (2007) Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites. J Ethnopharmacol 113:248–251
Freitas SF, Shinobara L, Sforcin JM, Guimaraes S (2006) In vitro effect of propolis on Giardia duodenalisTrophozoites. Phytomedicine 13(3):170–175
Gardner TB, Hill DR (2001) Treatment of giardiasis. Clin Microbiol Rev 14:114–128
Gardner WA Jr, Culberson DE, Bennett BD (1986) Trichomonas vaginalis in the prostate gland. Arch Pathol Lab Med 110(5):430–432
Haloci E, Manfredini S, Toska V, Vertuani S, Ziosi P, Topi I, Kolani H (2012) Antibacterial and antifungal activity assessment of Nigella sativa essential oils. World Acad Sci Eng Technol 66:1198–1200
Harris JC, Plummer S, Turner MP, Lloyd D (2000) The microaerophilic flagellate Giardia intestinalis: Allium sativum (garlic) is an effective antigiardial. Microbiology 146:3119–3127
Harzallah HJ, Noumi E, Bekir K, Bakhrouf A, Mahjoub T (2012) Chemical composition, antibacterial and antifungal properties of Tunisian Nigella sativa fixed oil. Afr J Microbiol Res 6(22):4675–4679
Hook EW III (1999) Trichomonas vaginalis-no longer a minor STD. Sex Trans Dis 26:388–389
Houcher Z, Boudiaf K, Benboubetra M, Houcher B (2007) Effects of methanolic extract and commercial oil of Nigella sativa on blood glucose and antioxidant capacity in alloxan-induced diabetic rats. Pteridines 18:8–18
Kassem HH, Majoud OA (2006) Trichomoniasis among women with vaginal discharge in Benghazi city, Libya. J Egypt Soc Parasitol 36:1007–1016
Khan MA, Ashfaq MK, Zuberi HS, Mhmood MS, Gilani AH (2003) The in vitro antifungal activity of aqueous extract from Nigella sativa seeds. Phytother Res 17(2):183–186
Klebanoff MA, Carey JC, Hauth JC, Hillier SL, Nugent RP, Thom EA, Ernest JM, Heine RP, Wapner RJ, Trout W, Moawad A, Leveno KJ, Miodovnik M, Sibai BM, Van Dorsten JP, Dombrowski MP, O’Sullivan MJ, Varner M, Langer O, McNellis D, Roberts JM (2001) National Institute of Child Health and Human Development Network of Maternal-Fetal Medicine Units. Failure of metronidazole to prevent preterm delivery among pregnant women with asymptomatic Trichomonas vaginalis infection. N Engl J Med 345:487–493
Lewis DA, Habgood L, White R, Barker KF, Murphy SM (1997) Managing vaginal trichomoniasis resistant to high-dose metronidazole therapy. Int J STD AIDS 8:780–784
Mahdi NK, Gany ZH, Sharief M (2001) Risk factors for vaginal trichomoniasis among women in Basra, Iraq. East Mediterr Health J 7:918–924
Meingasser JG, Thurner J (1979) Strain of Trichomonas vaginalis resistant to metronidazole and other 5-nitroimidazoles. Antimicrob Agents Chemother 15:254–257
Morsy TA, El Dasouki ST (1979) A study on vaginal trichomoniasis in Amman, Jordan. J Egypt Soc Parasitol 8:279–282
Negm AY, El-Haleem DA (2004) Detection of trichomoniasis in vaginal specimens by both conventional and modern molecular tools. J Egypt Soc Parasitol 34:589–600
Palmas C, Wakelin D, Gabriele F (1984) Transfer of immunity against Hymenolepis nana in mice with lymphoid cells or serum from infected donors. Parasitol 89:287–293
Rohrer L, Winterhalter KH, Eckert J, Köhler P (1986) Killing of Giardia lamblia by human milk is mediated by unsaturated fatty acids. Antimicrob Agents Chemother 30:254–257
Rojas JJ, Ochoa VJ, Ocampo S, Muñoz JF (2006) Screening for antimicrobial activity of ten medicinal plants used in Colombian Folkovic medicine: a possible alternative in treatment of non nosocomial infection. BMC Complement Altern Med 6:2
Rughooputh S, Greenwell P (2005) Trichomonas vaginalis: paradigm of a successful sexually transmitted organism. Br J Biomed Sci 62(4):193–200
Schwebke JR, Burgess D (2004) Trichomoniasis. Clin Microbiol Rev 17:794–803
Shaaban MT, El Silk SE, Tayel MA (2011) Efficiency of some plant extracts, carbohydrates and inorganic salts as anti-adhesion agents against the adhesion of Staphylococcus strains to HEp-2 cells. Life Sci J 8:1172–1182
Sink JD, Walkins SL, Zeigler JH, Miller RC (1964) Analysis of fat by gas liquid chromatography. J Anim Sci 23:111–121
Stenz L, François P, Fischer A, Huyghe A, Tangomo M, Hernandez D, Cassat J, Linder P, Schrenzel J (2008) Impact of oleic acid (cis-9-octadecenoic acid) on bacterial viability and biofilm production in Staphylococcus aureus. FEMS Microbiol Lett 287:149–155
Sun CQ, O’Connor CJ, Roberton AM (2003) Antibacterial actions of fatty acids and monoglycerides against Helicobacter pylori. FEMS Immunol Med Microbiol 36:9–17
Swygard H, Se˜na AC, Hobbs MM, Cohen MS (2004) Trichomoniasis: clinical manifestations, diagnosis, and management. Sex Transm Infect 80:91–95
Tennekoon K, Jeevathayaparan S, Kurukulasooriya A, Karunanayake E (1991) Possible hepatotoxicity of Nigella sativa seeds and Dregeavolubilis leaves. J Ethnopharmacol 31:283–289
Tonkal AMD (2009) In vitro antitrichomonal effect of Nigella sativa aqueous extract and wheat germ agglutinin. JKAU Med Sci 16:17–34
Upcroft JA, Dunn LA, Wright JM, Benakli K, Upcroft P, Vanelle P (2006) 5-Nitroimidazole drugs effective against metronidazole-resistant Trichomonas vaginalis and Giardia duodenalis spp. Antimicrob Agents Chemother 50:344–347
Wendel KA, Workowski KA (2007) Trichomoniasis challenges to appropriate management. Clin Infect Dis 44:123–129
Wojtczak L, Więckowski MR (1999) The mechanisms of fatty acid induced proton permeability of the inner mitochondrial membrane. J Bioenerg Biomembr 31:447–455
Won SR, Hong MJ, Kim YM, Li CY, Kim JW, Rhee HI (2007) Oleic acid: an efficient inhibitor of glucosyltransferase. FEBS Lett 581:4999–5002
World Health Organization (2001) Global prevalence and incidence of selected curable sexually transmitted infections: overview and estimates. WHO, Geneva (Switzerland). http://www.who.int/hiv/pub/sti/who_hiv_aids_2001.02.pdf
World Health Organization (2008) Global incidence and prevalence of selected curable sexually transmitted infections-2008. World Health Organization, Geneva, Switzerland. http://apps.who.int/iris/bitstream/10665/75181/1/9789241503839_eng.pdf
Zribi M, Mansour KB, Abid F, Fendri C, Masmoudi A (2008) Syndromic approach in Tunisian women: bacteriological validation. Int J STD AIDS 19:112–114
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Mahmoud, M.A.EF.A., Aminou, H.A. & Hashem, H.A. Are the fatty acids responsible for the higher effect of oil and alcoholic extract of Nigella sativa over its aqueous extract on Trichomonas vaginalis trophozoites?. J Parasit Dis 40, 22–31 (2016). https://doi.org/10.1007/s12639-014-0479-6
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DOI: https://doi.org/10.1007/s12639-014-0479-6