Microscopical and serological studies on Sarcocystis infection with first report of S. cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt


This study was performed for the purpose of investigating the prevalence and the species composition of Sarcocystis spp. in buffaloes in Assiut province, Egypt. Macroscopically we reported the infection of buffaloes with Sarcocystis fusiformis, while microscopically three Sarcocystis species (Sarcocystis cruzi, Sarcocystis levinei and Sarcocystis hominis) cysts were recognized, and were differentiated by their morphological features using both histopathological sections and electron microscope scanning. Regarding the prevalence of Sarcocystis species among buffaloes in Assiut province, we reported that, using gross examination of 90 buffaloes’ esophagus, only 23 samples out of 90 (25.5 %) were found to be infected; on the other hand, by using microscopical examination, the prevalence was 27.7 % (25 samples out of 90 samples were found to be infected). Using ELISA, 85 samples out of 90 (94.4 %) were found positive, an overall prevalence of 94.4 %. In this work we concluded that customary meat inspection methods in abattoirs in Egypt are insufficient for detecting Sarcocystis infection. Due to the presence of hidden or microscopic cysts, we strongly recommend the use of combined microscopical examination and ELISA for Sarcocystis diagnosis, to avoid human infection of such zoonotic parasite and to control the consequent disease. In addition, this study introduced the first report of S. cruzi in buffaloes in Egypt, and proved the hypothesis that S. cruzi is able to use animals such as water buffalo as intermediate hosts.

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  1. Abdel Rahman SM (2001) Serodiagnosis of two zoonotic parasites (Toxoplasma & Sarcocystis) in cattle. First Congress of Food Hygiene & Human Health, Faculty of Veterinary Medicine, Assiut, Egypt

  2. Bancroft JD, Stevens A (1993) Theory and practice of histological techniques, 3rd edn. Long Man Group Limited, London

    Google Scholar 

  3. Bhatia BB (2000) Textbook of veterinary protozoology, 1st edn. ICAR, New Delhi

    Google Scholar 

  4. Coles EH (1986) Veterinary clinical pathology, 4th edn. Saunders Company, Philadelphia

    Google Scholar 

  5. Collier L, Balows A, Sussman M (1998) Sarcocystis, Isospora and Cyclospora. In: Gransden WR (ed) Topley and Welson’s: microbiology and microbial infections, vol 5, 9th edn. Oxoford University Press Inc., New York, pp 319–326

    Google Scholar 

  6. Dubey JP, Speer CA, Fayer R (1989) Sarcocystosis of animals and man. C.A.B. Press, Boca Raton, p 105

    Google Scholar 

  7. Dubey JP, Hamir AN, Niezgoda M, Rupperch CE (1996) A Sarcocystis neurona-like organism associated with encephalitis in a stripped stuck (Mephitis mephitis). J Parasitol 82:172–174

    PubMed  Article  CAS  Google Scholar 

  8. El-Dakhly KM, El-Nesr KA, El-Nahass el-S, Hirata A, Sakai H, Yanai T (2011) Prevalence and distribution patterns of Sarcocystis spp. in buffaloes in Beni-Suef, Egypt. Trop Anim Health Prod 43:1549–1554

    PubMed  Article  Google Scholar 

  9. El-Nazer M, Abdel-Azim AH (2000) Seropositivity to Sarcocystis antigen in attendants of rheumatology clinic in Sohag University Hospital. South Valley Med J 4(2):145–155

    Google Scholar 

  10. Fatma GS, Maha SIS, Mohsen IA, Hoda MK (2008) Sarcocystis infection in cattle at Assiut abattoir: microscopical and serological studies. Ass Univ Bull Environ Res 11:47–58

    Google Scholar 

  11. Fawaz AA (1998) Incidence of Toxoplasma and Sarcosporidia in slaughtered animals in Qena Governorate. PhD thesis. Faculty of Veterinary Medicine, Assiut University

  12. Habeeb YS, Selim MA, Ali MM, Mahmoud LA, Abdel-Hady AM, Shfei A (1996) Serological diagnosis of extra-intestinal sarcocystosis. J Egypt Soc Parasitol 26(2):393–400

    PubMed  CAS  Google Scholar 

  13. Huong LT (1999) Prevalence of Sarcocystis spp. in water buffaloes in Vietnam. Vet Parasitol 86:33–39

    PubMed  Article  CAS  Google Scholar 

  14. Jain PC, Shah HL (1985) Cross-transmission studies of Sarcocystis cruzi of the cattle to the buffalo calves. Indian J Anim Sci 55:27–28

    Google Scholar 

  15. Jehle C, Dinkel A, Sander A, Morent M, Romig T, Luc PV, De TV, Thai VV, Mackenstedt U (2009) Diagnosis of Sarcocystis spp. Vet Parasitol 166:314–320

    PubMed  Article  CAS  Google Scholar 

  16. Juyal PD, Bhatia BB (1989) Sarcocystosis: an emerging zoonosis. Indian Vet Med J 13:66–69

    Google Scholar 

  17. Khalifa RM, El-Nadi NA, Sayed FG, Omran EK (2008) Comparative morphological studies on three Sarcocystis species in Sohag, Egypt. J Egypt Soc Parasitol 38(2):599–608

    PubMed  Google Scholar 

  18. Latif BM, Al-Delim JK, Mohamed BS, Al-Bayati SM, Al-Amiry AM (1999) Prevalence of Sarcocystis spp. in meat producing animals in Iraq. Vet Parasitol 84:85–90

    PubMed  Article  CAS  Google Scholar 

  19. Mohanty BN, Misra SC, Panda DN, Panda MR (1995) Prevalence of Sarcocystis infection in ruminants in Orissa. Indian Vet J 72:1026–1030

    Google Scholar 

  20. Morsy TA, Abdel-Mawla MM, Salama MM, Hamdi KN (1994) Assessment of intact Sarcocystis cystozoite as an ELISA antigen. J Egypt Soc Parasitol 24(1):85–91

    PubMed  CAS  Google Scholar 

  21. Mowafy NM (1993) Sarcosporidiosis in rodents. Ph.D. Thesis in Medical Science, Faculty of Medicine, Minia University, Egypt

  22. Said MS (1996) Muscular parasites in slaughtered animals in Assiut Governorate. PhD thesis, Faculty of Veterinary Medicine Assiut University

  23. Soulsby EJL (1982) Helminths, Arthropods and Protozoa of domesticated animals, 6th edn. Baillier, Tindall and Casell, London

    Google Scholar 

  24. Wang MQ, Lin QW, Liu HH, Xiao NB, Zhang GC, Gong FZ (1992) Comparative ultrastructural studies on the cysts of Sarcocystis cruzi in cattle and buffalo after cross-infections. Acta Veterinaria ET Zootechnica Sinica 23:347–353

    Google Scholar 

  25. Xiao NB, Zeng ND, Zhang CG, Wang M, Li Y, Gong FZ (1993) Development of Sarcocystis cruzi in buffalo (Bubalus bubalis) and cattle (Bos Taurus). Acta Veterinaria et Zootechnica Sinica 24:185–192

    Google Scholar 

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Correspondence to Mahmoud R. Abd Ellah.

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Metwally, A.M., Abd Ellah, M.R., AL-Hosary, A.A. et al. Microscopical and serological studies on Sarcocystis infection with first report of S. cruzi in buffaloes (Bubalus bubalis) in Assiut, Egypt. J Parasit Dis 38, 378–382 (2014). https://doi.org/10.1007/s12639-013-0257-x

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  • Buffaloes
  • Sarcocystis
  • Assiut
  • S. cruzi