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Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus

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Abstract

The present observation records the lipid classes and their fatty acid composition of the neutral lipid and phospholipid of the trematode Paramphistomum cervi and liver of its host Capra hircus, the common Indian goat. Thin Layer Chromatography and Gas Liquid Chromatography were used to identify different neutral lipid and phospholipid components. The results show that among the neutral lipid fractions, the amount of combined hydrocarbon, wax ester and steryl ester is more or less equal in parasite and its host, but the percent of triacylglycerol is more in host liver than the parasite and the percent of total sterol is more in parasite than that of its host liver. Among the phospholipid fractions, phosphatidylinositol and phosphatidylethanolamine in the trematode parasite P. cervi and phosphatidylcholine and cardiolipin in the host’s liver are the major components. The predominant fatty acids in the neutral lipid and phospholipid fractions of both the host liver and the trematode P. cervi include C16:0, C18:0, C18:1, and C18:2. The results reveal that the parasite P. cervi take up almost all the lipid and fatty acids from their host, which is required for their life cycle.

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References

  • Ackman RG, Burgher RD (1965) Cod liver oil fatty acids as secondary reference standards in the GLC of polyunsaturated fatty acids of animal origin: analysis of a dermal oil of the Atlantic leather-back turtle. J Am Oil Chem Soc 42:38–42

    Article  CAS  PubMed  Google Scholar 

  • Awharitoma AO, Opute FI, Ali SN, Obiamiwe BA (1988) Lipid composition of four species of amphistomes (trematoda) from the rumen of cattle. Int J Parasitol 18:441–444

    Article  CAS  PubMed  Google Scholar 

  • Bankov I, Timanova A, Barrett J (1998) Sphingomyelin synthesis in helminths: a minireview. Folia Parasitol 45:257–260

    CAS  PubMed  Google Scholar 

  • Barrett J (1981) Biochemistry of parasitic helminths. Macmillan, London

    Google Scholar 

  • Barrett J, Cain GD, Fairbairn D (1970) Sterols in Ascaris lumbricoides (Nematoda), Macracan thorhynchus hirudinaceus and Moniliformis dubius (Acanthocephala) and Echinostoma revolutum (Trematoda). J Parasitol 56:1004–1008

    Article  CAS  PubMed  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  PubMed  Google Scholar 

  • Brito CFA, Oliveira GC, Oliveira SC, Street M, Riengrojpitak S, Wilson RA, Simpson AJG, Oliveira RC (2002) Sm 14 gene expression in different stages of the Schistosoma mansoni life cycle and immunolocalization of the Sm 14 protein within the adult worm. Braz J Med Biol Res 35:377–381

    Article  CAS  PubMed  Google Scholar 

  • Brouwers JF, Smeenk IM, van Golde LM, Tielens AG (1997) The incorporation, modification and turnover of fatty acids in adult Schistosoma mansoni. Mol Biochem Parasitol 88:175–185

    Article  CAS  PubMed  Google Scholar 

  • Brouwers JF, Van Hellemond JJ, van Golde LM, Tielens AG (1998) Ether lipids and their possible physiological function in adult Schistosoma mansoni. Mol Biochem Parasitol 96:49–58

    Article  CAS  PubMed  Google Scholar 

  • Christie WW (2003) Lipid analysis, 3rd edn. Oily Press, Bridgwater

    Google Scholar 

  • Conn EE, Stumpf PK, Bruening G, Doi RH (2003) Outlines of biochemistry, 5th edn. John Wiley, Singapore

    Google Scholar 

  • Dalton JP, Skelly P, Halton DW (2004) Role of the tegument and gut in nutrient uptake by parasitic platyhelminthes. Can J Zool 82:211–232

    Article  Google Scholar 

  • Furlong ST (1991) Unique roles for lipids in Schistosoma mansoni. Parasitol Today 7:59–62

    Article  CAS  PubMed  Google Scholar 

  • Furlong ST, Thibault KS, Rogers RA (1992) Fluorescent phospholipids preferentially accumulate in sub-tegumental cells of schistosomula of Schistosoma mansoni. J Cell Sci 103:823–830

    CAS  PubMed  Google Scholar 

  • Gallo GJ, Fried B (1984) Association of particular systems with the release of neutral lipids in Echinostoma revolutum (Trematoda) adults. J Chem Ecol 10:1065–1069

    Article  CAS  PubMed  Google Scholar 

  • Gardocki ME, Jani N, Lopes JM (2005) Phosphatidylinositol biosynthesis: biochemistry and regulation. Biochimi Biophys Acta 1735:89–100

    Article  CAS  Google Scholar 

  • Ghosh D, Misra KK (2011) Major lipids and fatty acids in the liver and rumen fluid of the goat (Capra hircus) infected with the trematode Paramphistomum cervi. J Helminthol 85:246–254

    Article  CAS  PubMed  Google Scholar 

  • Grunwald C (1978) Function of sterols. Phil Trans R Soc Lond B 284:541–558

    Article  CAS  Google Scholar 

  • Halton DW (1967) Observations on the nutrition of digenetic trematodes. Parasitology 57:639–660

    Article  CAS  PubMed  Google Scholar 

  • Humiczewska M, Rajski K (2005) Lipids in the host-parasite system: digestive gland of Lymnaea truncatula infected with the developmental stages of Fasciola hepatica. Acta Parasitol 50:235–239

    Google Scholar 

  • Mangold HK (1969) Aliphatic lipids. In: Stahl E (ed) Thin-layer chromatography. Springer, New York, pp 363–421

    Chapter  Google Scholar 

  • Marsit CJ, Fried B, Sherma J (2000) Neutral lipids in cercariae, encysted metacercariae and rediae of Zygocotyle lunata. J Parasitol 86:1162–1163

    Article  CAS  PubMed  Google Scholar 

  • Meyer F, Kimura S, Mueller JL (1966) Lipid metabolism in the larval and adult forms of the tapeworm Spirometra mansonoides. J Biol Chem 241:4224–4232

    CAS  PubMed  Google Scholar 

  • Misra S, Ghosh A (1991) Analysis of epicuticular waxes. In: Linskens HF, Jackson JF (eds) Essential oils and waxes. Springer, Berlin, pp 205–229

    Chapter  Google Scholar 

  • Misra S, Ghosh A, Dutta J (1984) Production and composition of microbial fat from Rhodotorula glutinis. J Sci Food Agric 35:59–65

    Article  CAS  Google Scholar 

  • Oldenborg V, van Vugt F, van Golde LMG (1975) Composition and metabolism of phospholipid of Fasciola hepatica, the common liver fluke. Biochim Biophys Acta 398:101–110

    Article  CAS  PubMed  Google Scholar 

  • Payrastre B (2004) Phosphoinositides. In: Nicolaou A, Kokotos G (eds) Bioactive lipids. The Oily Press, Bridgwater, pp 63–84

    Google Scholar 

  • Pearce EJ, Sher A (1989) Three major surface antigens of Schistosoma mansoni are linked to the membrane by glycosylphosphatidylinositol. J Immunol 142:979–984

    CAS  PubMed  Google Scholar 

  • Redman CA, Kennington S, Spathopoulou T, Kusel JR (1997) Interconversion of sphingomyelin and ceramide in adult Schistosoma mansoni. Mol Biochem Parasitol 90:145–153

    Article  CAS  PubMed  Google Scholar 

  • Roberts LS, Schmidt G, Janovy J Jr (2000) Foundations of parasitology, 6th edn. Mc-Graw Hill, International Edition, Boston

    Google Scholar 

  • Robinson BS, Hii CS, Poulos A, Ferrante A (1997) Activation of neutral sphingomyelinase in human neutrophils by polyunsaturated fatty acids. Immunology 91:274–280

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Rouser G, Kritchevsky G, Yamamoto A (1976) Column chromatographic and associated procedures for separation and determination of phosphatides and glycolipids. In: Marinetti GV (ed) Lipid chromatographic analysis, vol 3, 2nd edn. M Dekker, New York, pp 713–776

    Google Scholar 

  • Sato S, Hirayama T, Hirazawa N (2008) Lipid content and fatty acid composition of the monogenean Neobenedenia girellae and comparison between the parasite and host fish species. Parasitology 135:967–975

    Article  CAS  PubMed  Google Scholar 

  • Smith TM, Doughty BL, Brown JN (1977) Fatty acid and lipid class composition of Schistosoma japonicum eggs. Comp Biochem Physiol Part B: Comparative Biochemistry 57:59–63

    Article  CAS  Google Scholar 

  • Smyth JD (1994) Animal parasitology, 3rd edn. Cambridge University Press, Cambridge

    Google Scholar 

  • Smyth JD, Halton DW (1983) The physiology of trematodes, 2nd edn. Cambridge University Press, Cambridge

    Google Scholar 

  • Sweely CC, Bentley R, Makita M, Wells WW (1963) Gas-liquid chromatography of trimethylsilyl derivatives of sugars and related substances. J Am Oil Chem Soc 85:2497–2500

    Article  Google Scholar 

  • Tallima H, Ridi RE (2005) Methyl-β-cyclodextrin treatment and filipin staining reveal the role of cholesterol in surface membrane antigen sequestration of Schistosoma mansoni and S. haematobium lung stage larvae. J Parasitol 91:720–725

    Article  CAS  PubMed  Google Scholar 

  • Tarr GE (1973) Comparison of ascarosides from four species of ascarid and one of oxurid (Nematoda). Comp Biochem Physiol 46B:167–176

    Google Scholar 

  • Tielens AGM (1997) Biochemistry of trematodes. In: Fried B, Graczyk TK (eds) Advances in trematode biology. CRC Press, Boca Raton, pp 309–343

    Google Scholar 

  • Tielens AGM (1999) Metabolism. In: Dalton JP (ed) Fasciolosis. CABI, Wallingford, pp 277–306

    Google Scholar 

  • Vykhrestyuk NP, Yarygina GV (1982) Preliminary studies of lipids of the trematodes Eurytrema pancreaticum, Calicophoron erschowi and the turbellarian Penecurva sibirica. Mol Biochem Parasitol 5:221–229

    Article  CAS  PubMed  Google Scholar 

  • Worgall TS, Johnson RA, Seo T, Gierens H, Deckelbaum RJ (2002) Unsaturated fatty acid- mediated decreases in sterol regulatory element- mediated gene transcription are linked to cellular sphingolipid metabolism. J Biol Chem 277:3878–3885

    Article  CAS  PubMed  Google Scholar 

  • Wu R, Chen L, Yu Z, Quinn PJ (2006) Phase diagram of stigmasterol- dipalmitoylphosphatidylcholine mixtures dispersed in excess water. Biochim Biophysic Acta 1758:764–771

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are thankful towards the Principal, R.B.C College, Naihati for providing the required laboratory facilities and to Dr. A. Ghosh, DRDC, Kolkata for extending help towards GLC analysis.

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  1. Department of Zoology, R.B.C. College, Naihati, 743 165, West Bengal, India

    Debasree Ghosh & K. K. Misra

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  1. Debasree Ghosh
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  2. K. K. Misra
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Correspondence to K. K. Misra.

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Ghosh, D., Misra, K.K. Comparison of fatty acid contents of the neutral and phospholipids of the trematode Paramphistomum cervi and liver of its host, Capra hircus . J Parasit Dis 38, 223–232 (2014). https://doi.org/10.1007/s12639-012-0229-6

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  • DOI: https://doi.org/10.1007/s12639-012-0229-6

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