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Serodetection of Ehrlichia canis infection in dogs from Ludhiana district of Punjab, India

Journal of Parasitic Diseases volume 35, pages 195–198 (2011)Cite this article

Abstract

Dot-ELISA (enzyme-linked immunosorbent assay) Immunocomb® assay was conducted to detect the presence of antibodies against Ehrlichia canis in blood samples of 60 privately owned dogs suspected to be infected with E. canis from the Small Animal Clinics, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, Punjab (India). Antibodies reactive to E. canis were detected in plasma in 48 samples out of 60 samples by Immunocomb® Dot-ELISA. Out of these 39.58% samples were low positive (Titre 1:20–1:40), 31.25% were medium positive (Titre 1:80–1:640) and 29.16% were high positive (Titre >1,280), for the infection. When examined by microscopy, only two samples revealed typical E. canis morulae. Haemato-cellular examination revealed thrombocytopenia along with anaemia and leucopenia. Results suggest that E. canis infection circulates in dogs in India in low non-detectable numbers by microscopy and is transmitted by the brown dog tick Rhipicephalus sanguineus.

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Acknowledgments

Thanks are due to The Director of Research, Guru Angad Dev Veterinary and Animal Sciences University for providing finances for conduction of work. Special thanks are due to Dr. Mohamed S. M. Eljadar, M.V.Sc. student of Veterinary Parasitology Department for clicking the photographs.

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Authors and Affiliations

  1. Department of Veterinary Parasitology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, India

    L. D. Singla, Harkirat Singh, P. Kaur, N. K. Singh & P. D. Juyal

  2. Department of Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, 141004, India

    N. D. Singh

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  1. L. D. Singla
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  2. Harkirat Singh
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  3. P. Kaur
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  4. N. D. Singh
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  5. N. K. Singh
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  6. P. D. Juyal
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Correspondence to L. D. Singla.

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Singla, L.D., Singh, H., Kaur, P. et al. Serodetection of Ehrlichia canis infection in dogs from Ludhiana district of Punjab, India. J Parasit Dis 35, 195–198 (2011). https://doi.org/10.1007/s12639-011-0055-2

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  • DOI: https://doi.org/10.1007/s12639-011-0055-2

Keywords

  • Dogs
  • Dot-ELISA
  • Ehrlichia canis
  • Immunocomb
  • Pancytopenia