Journal of Parasitic Diseases

, Volume 33, Issue 1–2, pp 65–69 | Cite as

Histopathological changes of the esophagus of the freshwater eels, Macrognathus aculeatus (Bl.) and Mastacembelus pancalus (Lac.) due to infection of metacercariae of Clinostomum mastacembeli Jaiswal, 1957

Original Article


Macrognathus aculeatus (Bl.) and Mastacembelus pancalus (Lac.) (Family: Mastacembelidae) are considered to be the most delicious fish in Andhra Pradesh due to the tenderness of their muscles. The metacercariae of Clinostomum mastacembeli Jaiswal, 1957 are found infesting the oesophagus of these fishes heavily. During present studies, histopathological changes caused due to the encystment of metacercariae of C. mastacembeli in the oesophagus of these fishes were observed. The four layers of the oesophagus namely; serosa, muscualris layer, submucosa and mucosa were found severely affected. Histopathological changes included decrease in the lumen diameter of the oesophagus, atrophy and necrosis of epithelial cells of serosa layer, shrinkage of villi due to thickening of muscularis layer and formation of small vacuoles in the sub-mucosal layers.


Clinostomum mastacembeli Histopathology Macrognathus aculeatus Mastacembelus pancalus Esophagus 


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  1. Bancraft JD (1975) Histochemical Techniques. Butterworths, London and BostonGoogle Scholar
  2. Chandler AC (1951) Studies on the metacercariae of Perca flavescens in Lake Itasca Minnesota. Am Midl Nat, 45: 711–721CrossRefGoogle Scholar
  3. Chapman JA, Hunter GWIII (1954) Studies on the host-parasite reactions. VIII. The pigment cells surrounding the metacercarial cysts of Cryptocotyle lingua in the cunner, Tautogolabrus adspersus (Walbaum). Trans Am Microsc, Soc 73:28–36CrossRefGoogle Scholar
  4. Faust EC, Khaw OW (1925) Excystment phenomenon in Clonorchis sinensis. Proc Soc Exp Biol Med, 23:245–248Google Scholar
  5. Gupta AK, Agarwal SM (1983a) Host-parasite relationship in Channa punctatus and Euclinostomum heterostomum. Current Science, 52:474–476Google Scholar
  6. Gupta AK, Agarwal SM (1983b) Host-parasite relationship in Channa punctatus and Euclinostomum heterostomum II. Current Science, 52:1141–1143Google Scholar
  7. Gupta AK, Agarwal SM (1984) Host-parasite relationship in Channa punctatus and Euclinostomum heterostomum III. Transaminases, total proteins and amino acids. Current Science, 53:710–711Google Scholar
  8. Hiral H, Ooiso H, Kifune T, Kiyato T and Sakaguchi Y (1987) Clinostomum complanatum infection in posterior wall of pharynx of human. Japenese J Parasitol, 36:142–144Google Scholar
  9. Hoffman GL (1967) Synopsis of Strigeoidea (Trematoda) of Fishes and their life-cycles. Fish Bull 175, United States Department of the Interior Fish and Wild Life Service Washington 60: 439–469Google Scholar
  10. Hoffman GL, Dunbar CE (1963) Studies on Neogagatea kentuckiensis (Cable, 1935) n. comb. (Trematoda: Strigeoidea: Cyathocotylidae) J Parasitol, 49:737–744CrossRefGoogle Scholar
  11. Hunter GW III, Dalton HC (1939) Studies on Clinostomum V The cyst of the yellow grub of fish (Clinostomum marginatum) Proc Helminth Soc Wash, 6:73–76Google Scholar
  12. Hunter GW III, Hunter WS (1940) Studies on the development of the metacercaria and the nature of the cyst of Posthodiplostomum minimum (MacCallum, 1921) (Trematoda: Strigeata) Trans Am Microsc Soc, 59:52–63CrossRefGoogle Scholar
  13. Isobe A, Kinoshita S, Hojo N, Fukushima T, Shiwaku K, Yamane Y (1994) The 12th human case of Clinostomum sp. infection in Japan. Japense J Parasitol, 43:193–198Google Scholar
  14. Kalantan AMN, Arfin M, Nizami WA (1986) Histochemical nature and origin of the metacercarial cyst of Clinostomum complanatum (Trematoda: Digenea) Trans Amer Mircoscop Soc 105:290–295CrossRefGoogle Scholar
  15. Kalantan AMN, Arfin M and Nizami WA (1987) Seasonal incidence and pathogenicity of metacercaria of Clinostomum complanatum (Trematoda: Digenea) in Aphanius dispar. Japenese Journal of Parasitology, 36:17–23Google Scholar
  16. Laxma Reddy BG, Benarjee G, Rajendra V, Bikshapathi M, Swamy M, Rama Rao NJ (2006) Histopathological and histochemical abnormalities induced by Euclinostomum heterostomum in the liver of freshwater fish, Channa punctatus. J Aqua Biol, 21(2):1–5Google Scholar
  17. Lo C, Wang C, Kou G (1992) The pathology of loach (Misgrunus anguillicaudatus) infected with Clinostomum complanatum (Rudolphi, 1814) Acta Zoologica Tiawan, 3:145–154Google Scholar
  18. Maejima J, Fukumoto S, Tanihata T, Wang HR, Hirai K (1996) The fourth case of human infection with Clinostomum sp. (Trematoda: Clinostomatidae) in Shimane Prefecture. Jap J Parasitol, 45:333–337Google Scholar
  19. Mahdy O (1991) Morphological studies on the role of some freshwater fishes in transmitting parasitic helminthes of some avian hosts. Ph.D Thesis (Parasitology), Faculty of Veterinary Medicine Cairo University, EgyptGoogle Scholar
  20. Mahdy O, Manal A, Essa A, Easa MElS (1995) Parasitological and pathological studies on heterophyid infections in Tilapia sp. from Manzala lake, Egypt Egyptian J Comparative Pathol and Clinic Pathol, 8:131–145.Google Scholar
  21. Mahmoud N, Youssef H, Khalifa R, Nassar A (1989) Studies on metacercarial infection in fresh water fish in Assuit province. Egyptian J Comparative Pathol and Clinic Pathol, 2:213–224Google Scholar
  22. Malek M, Mobedi I (2001) Occurrence of Clinostomum complanatum (Rudolpi, 1819) (Digenea: Clinostomatidae) in Capoeta capoeta gracilis (Osteichthyes: Cyprinidae) from Shiroud River, Iran. Iranian J Publ Health, 30:95–98Google Scholar
  23. Mawdesley-Thomas LE, Young PC (1967) Cutaneous melanosis in a flounder (Platychthys flesus L) Vet Rec, 81:384–385Google Scholar
  24. Orecka-Grabda T (1991) Haemato- and histopathological changes in the whitefish (Coregonus i Linn.) invaded by metacercariae of Cotylurus erraticus (Syn. Ichthyocotylurus) (Rudolphi, 1809) Fish Pathology, 221:3–19Google Scholar
  25. Osborn HL (1911) On the distribution and occurrence in the United States and Canada of Clinostomum marginatum a trematode parasite in fish and birds. Biol Bull, 20:350–366CrossRefGoogle Scholar
  26. Paperna I, Vanas JG (1983) The pathology of Chilodenella hexastica infections in cichlid fishes. Journal of Fish Biology, 23(4):441–450CrossRefGoogle Scholar
  27. Pearse AGE (1968) Histochemistry. Theoretical and Applied. 2nd Edition, Little Brown and Company, Boston, MSSGoogle Scholar

Copyright information

© Indian Society for Parasitology 2009

Authors and Affiliations

  1. 1.Department of Animal SciencesYogi Vemana UniversityKadapaIndia
  2. 2.Department of ZoologyAndhra UniversityVisakhapatnamIndia

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