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Silicon Nanoparticles on the Enhanced Micropropagation of Hemidesmus indicus (L.) R. Br. ex Schult

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Abstract

Silicon nanoparticles (SiNPs) in Murashige and Skoog (MS) medium improved micropropagation of Hemidesmus indicus (L.) R. Br. ex Schult. (Apocynaceae), a valuable and threatened medicinal plant. Nodal explant-derived shoots amplified (130 shoots/inoculum/culture vessel) on MS medium + 1.0 mg L−1 SiNPs and 1.0 mg L−1 6-benzylaminopurine (BAP) + 0.5 mg L−1 kinetin (Kn) + 0.1 mg L−1 indole-3-acetic acid (IAA). These shoots exhibited superior growth over control. The SiNPs-derived shoots developed functional stomata, cuticle, multi-layered mesophyll, hypodermis, increased density of cells in the cortex, and vascular elements in the leaf and stem. In contrast, shoots generated on the control were with non-functional stomata, underdeveloped mesophylls, and few dermal, ground, and vascular tissues. The shoots were amplified on SiNPs-amended medium rooted maximum (99%) than control (90.3%) on 1/4th MS + 3.0 mg L−1 IBA (Indole-3-butyric acid). The rooted plants were acclimatized with ease and successfully transferred to the field. It is suggested that the addition of 1.0 mg L−1 SiNPs in the cultivation medium induced micro-morpho-anatomical features improving the micropropagation of H. indicus.

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Funding

The work was financially supported by the Researchers Supporting Project (Number RSP-2023R86), King Saud University, Riyadh, Saudi Arabia.

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Authors and Affiliations

  1. Biotechnology Unit, Kanchi Mamunivar Government Institute for Postgraduate Studies and Research, Puducherry, 605 008, India

    M. Manokari & Mahipal S. Shekhawat

  2. Department of Botany, Siddha Clinical Research Unit, Central Council for Research in Siddha, Palayamkottai, Tamil Nadu, 627 002, India

    M. Manokari

  3. Department of Botany & Microbiology, College of Science, King Saud University, P.O. Box 2455, 11451, Riyadh, Saudi Arabia

    Mohammad Faisal & Abdulrahman A. Alatar

  4. CEREGE, Aix-Marseille University, CNRS, IRD, INRAE, Aix-en-Provence, France

    Doris Barboni

  5. French Institute of Pondicherry, UAR3330 CNRS-MEAE, Puducherry, India

    Doris Barboni

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  1. M. Manokari
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  2. Mohammad Faisal
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  3. Abdulrahman A. Alatar
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  4. Doris Barboni
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Contributions

Manokari M, Mahipal S Shekhawat, Mohammad Faisal, and Abdulrahman A. Alatar: Conceptualization, investigation, methodology. Manokari M and Mahipal S Shekhawat: Data compilation and hardening of the plants. Mahipal S Shekhawat, Manokari M, Mohammad Faisal, and Abdulrahman A. Alatar: Writing of original draft, statistics and data interpretation. Doris Barboni: Revision of the manuscript. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to Mahipal S. Shekhawat.

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Compliance with Ethical Standards

No, all testing are conducted in this study as per ASTM standards.

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Key message

The remarkable improvement in morphology and micro-structural traits of in vitro-raised shoots of H. indicus, achieved through the incorporation of silicon nanoparticles (SiNPs) in the nutrient medium, underscores the immense potential of this novel system for conservation and sustainable utilization of medicinal climber.

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Manokari, M., Faisal, M., Alatar, A.A. et al. Silicon Nanoparticles on the Enhanced Micropropagation of Hemidesmus indicus (L.) R. Br. ex Schult. Silicon 16, 775–782 (2024). https://doi.org/10.1007/s12633-023-02714-x

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