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Silicon Mitigates the Adverse Effect of Drought in Canola (Brassica napus l.) Through Promoting the Physiological and Antioxidants Activity

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Abstract

Drought is the major limiting factor for crops which reduces the growth and ultimately yield. However, silicon (Si) is considered one of the important elements for ameliorating adverse effect of abiotic stresses including drought also. To re-establish the previous evidence of Si, an experiment was carried out in a wire-house under pot-culture, where canola plants were grown under normal conditions (100% field capacity) and water deficit condition (40% field capacity). To know the ameliorative effect of silicon on drought-induced canola plants through promoting the physiological, growth, biochemical, and yield attributes of canola (Brassica napus L.); exogenous application of Si was done by two different methods i.e., through seed treatment (priming) before sowing and foliar spray at vegetative stage. For priming, seeds of canola were dipped for six hours in a 5 mM Si solution, whereas for foliar spray, three different levels of Si i.e., 2, 4 and 6 mM Si solution were applied at vegetative stage (after 40 days of seed emergence). Drought stress caused a substantial decrease in the parameters of water relations, and the uptake of various nutrients in canola plants. However, the application of Si significantly improved plants’ ability to withstand the drought stress through enhancing the uptake of Si in plants and also enhanced the activity of ascorbate peroxidase (APX), peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT). Data on, water potential, leaf water contents ratio and leaf pigments, (for example, chlorophyll a, chlorophyll b, total chlorophyll, carotenoids), was positively influenced by applying Si under water deficit stress. Similarly, agronomic parameters such as seeds pod−1, seed weight and seed yield were also enhanced as a result of the Si application under drought condition. Among these application methods of Si, the foliar spray has been found more effective for alleviating the adverse effects of water deficit stress on canola plants. Therefore, the results of the present study revealed that foliar application of Si at the vegetative stage may be useful for ameliorating the adverse effect of drought; since Si promoted to maintain turgor pressure, antioxidants activity in plants and also improved the nutrient accumulation both under water stress and control condition.

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References

  1. Waraich EA, Ahmad R, Ahmad R et al (2020) Comparative study of growth, physiology and yield attributes of camelina (Camelina sativa L.) and canola (Brassica napus L.) under different irrigation regimes. Pak J Bot 52:1–8. https://doi.org/10.30848/pjb2020-5(2)

    Article  CAS  Google Scholar 

  2. Ali AA, Areej AA, Fahad AA-Y et al (2017) Effect of combined biotic and abiotic stress on some physiological aspects and antioxidant enzymatic activity in mungbean (Vigna radiate L.). Afr J Agric Res 12:700–705. https://doi.org/10.5897/AJAR2016.12084

    Article  Google Scholar 

  3. Ahmad Z, Waraich EA, Akhtar S, Anjum S, Ahmad T, Mahboob W, Hafeez OB, Tapera T, Labuschagne M, Rizwan M (2018) Physiological responses of wheat to drought stress and its mitigation approaches. Acta Physiol Plant 40(4):80. https://doi.org/10.1007/s11738-018-2651-6

    Article  CAS  Google Scholar 

  4. Ahmad Z, Waraich EA, Ahmad R, Shahbaz M (2017) Modulation in water relations, chlorophyll contents and antioxidants activity of maize by foliar phosphorus application under drought stress. Pak J Bot 49:11–19

    CAS  Google Scholar 

  5. Gürel F, Öztürk ZN, Uçarlı C, Rosellini D (2016) Barley genes as tools to confer abiotic stress tolerance in crops. Front Plant Sci 7:1137. https://doi.org/10.3389/fpls.2016.01137

    Article  PubMed  PubMed Central  Google Scholar 

  6. EL Sabagh A, Hossain A, Barutçular C et al (2018) Sustainable maize (Zea mays L.) production under drought stress by understanding its adverse effect, survival mechanism and drought tolerance indices. J Exp Biol Agric Sci 6(2):282–295. https://doi.org/10.18006/2018.6(2).282.295

    Article  CAS  Google Scholar 

  7. Maghsoudi K, Emam Y, Ashraf M (2016) Foliar application of silicon at different growth stages alters growth and yield of selected wheat cultivars. J Plant Nutr 39:1194–1203. https://doi.org/10.1080/01904167.2015.1115876

    Article  CAS  Google Scholar 

  8. Waraich EA, Ahmad Z, Ahmad R, Saifullah, Ashraf MY (2015) Foliar applied phosphorous enhanced growth, chlorophyll contents, gas exchange attributes and PUE in wheat (Triticum aestivum L.). J Plant Nutr 38(12):1929–1943. https://doi.org/10.1080/01904167.2015.1043377

    Article  CAS  Google Scholar 

  9. Ahmad R, Waraich EA, Nawaz F, Ashraf MY, Khalid M (2016) Selenium (se) improves drought tolerance in crop plants - a myth or fact? J Sci Food Agric 96:372–380

    Article  CAS  Google Scholar 

  10. Bukhari MA, Ashraf MY, Ahmad R et al (2015) Improving drought tolerance potential in wheat (Triticum aestivum L.) through exogenous silicon supply. Pak J Bot 47:1641–1648

    CAS  Google Scholar 

  11. Mafakheri A, Siosemardeh A, Bahramnejad B et al (2010) Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aust J Crop Sci

  12. Kaya C, Tuna L, Higgs D (2006) Effect of silicon on plant growth and mineral nutrition of maize grown under water-stress conditions. J Plant Nutr 29:1469–1480. https://doi.org/10.1080/01904160600837238

    Article  CAS  Google Scholar 

  13. Habibi G (2015) Contrastive response of Brassica napus L. to exogenous salicylic acid, selenium and silicon supplementation under water stress. Arch Biol Sci 67(2):397–404. https://doi.org/10.2298/ABS140411006H

    Article  Google Scholar 

  14. Ahmad Z, Anjum S, Waraich EA, Ayub MA, Ahmad T, Tariq RMS, Ahmad R, Iqbal MA (2018) Growth, physiology, and biochemical activities of plant responses with foliar potassium application under drought stress–a review. J Plant Nutr 1(13):1734–1743. https://doi.org/10.1080/01904167.2018.1459688

    Article  CAS  Google Scholar 

  15. Mohtashami R, Movahhedi Dehnavi M, Balouchi H, Faraji H (2020) Improving yield, oil content and water productivity of dryland canola by supplementary irrigation and selenium spraying. Agric Water Manag 232:106046. https://doi.org/10.1016/j.agwat.2020.106046

    Article  Google Scholar 

  16. Ahmad Z, Waraich EA, Barutçular C et al (2020) Enhancing drought tolerance in wheat through improving morpho- physiological and antioxidants activities of plants by the supplementation of foliar silicon. https://doi.org/10.32604/phyton.2020.09143

  17. Ma JF (2004) Role of silicon in enhancing the resistance of plants to biotic and abiotic stresses. Soil Sci Plant Nutr 50(1):11–18. https://doi.org/10.1080/00380768.2004.10408447

    Article  CAS  Google Scholar 

  18. Rizwan M, Ali S, Ibrahim M, Farid M, Adrees M, Bharwana SA, Zia-ur-Rehman M, Qayyum MF, Abbas F (2015) Mechanisms of silicon-mediated alleviation of drought and salt stress in plants: a review. Environ Sci Pollut Res 22:15416–15431. https://doi.org/10.1007/s11356-015-5305-x

    Article  CAS  Google Scholar 

  19. Maghsoudi K, Emam Y, Pessarakli M (2016) Effect of silicon on photosynthetic gas exchange, photosynthetic pigments, cell membrane stability and relative water content of different wheat cultivars under drought stress conditions. J Plant Nutr 39:1001–1015. https://doi.org/10.1080/01904167.2015.1109108

    Article  CAS  Google Scholar 

  20. Fialová I, Šimková L, Vaculíková M, Luxová M (2018) Effect of Si on the Antioxidative defense of young maize roots under NaCl stress. Silicon. 10(6):2911–2914

    Article  Google Scholar 

  21. Adrees M, Ali S, Rizwan M, Zia-ur-Rehman M, Ibrahim M, Abbas F, Farid M, Qayyum MF, Irshad MK (2015) Mechanisms of silicon-mediated alleviation of heavy metal toxicity in plants: a review. Ecotoxicol Environ Saf 119:186–197. https://doi.org/10.1016/j.ecoenv.2015.05.011

    Article  CAS  PubMed  Google Scholar 

  22. Chance B (1955) Maehly AC (1955) assay of catalases and peroxidases. Methods Enzymol 2:764–775. https://doi.org/10.1016/S0076-6879(55)02300-8

    Article  Google Scholar 

  23. Cakmak I (1994) Activity of ascorbate-dependent H2O2-scavenging enzymes and leaf chlorosis are enhanced in magnesium- and potassium-deficient leaves, but not in phosphorus-deficient leaves. J Exp Bot 45:1259–1266. https://doi.org/10.1093/jxb/45.9.1259

    Article  CAS  Google Scholar 

  24. Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. Anal Biochem 161(2):559–566. https://doi.org/10.1016/0003-2697(87)90489-1

    Article  CAS  PubMed  Google Scholar 

  25. R Core Team (2014) R: a language and environment for statistical computing. R Found Stat Comput Vienna, Austria URL https://wwwR-project.org/

  26. Soleimanzadeh H (2012) Response of sunflower (Helianthus annuus L.) to selenium application under water stress. World Appl Sci J 17(9):1115–1119

    CAS  Google Scholar 

  27. Abdel-Motagally FMF, El-Zohri M (2018) Improvement of wheat yield grown under drought stress by boron foliar application at different growth stages. J Saudi Soc Agric Sci 17:178–185. https://doi.org/10.1016/j.jssas.2016.03.005

    Article  Google Scholar 

  28. Shi Y, Zhang Y, Han W, Feng R, Hu Y, Guo J, Gong H (2016) Silicon enhances water stress tolerance by improving root hydraulic conductance in Solanum lycopersicum L. Front Plant Sci 7:1–15. https://doi.org/10.3389/fpls.2016.00196

    Article  Google Scholar 

  29. Maghsoudi K, Emam Y, Ashraf M (2016) Foliar application of silicon at different growth stages alters growth and yield of selected wheat cultivars. J Plant Nutr 39:1194–1203. https://doi.org/10.1080/01904167.2015.1115876

    Article  CAS  Google Scholar 

  30. Ahmad Z, Waraich EA, Ahmad T et al (2015) Yield responses of maize as influenced by supplemental foliar applied phosphorus under drought stress. Int J Food Allied Sci 1:45. https://doi.org/10.21620/ijfaas.2015245-55

    Article  Google Scholar 

  31. Shirani Rad AH, Abbasian A, Aminpanah H (2013) Evaluation of rapeseed (Brassica napus L.) cultivars for resistance against water deficit stress. Bulg J Agric Sci 19(2):266–273

    Google Scholar 

  32. Dossa K, Yehouessi LW, Likeng-Li-Ngue BC, Diouf D, Liao B, Zhang X, Cissé N, Bell J (2017) Comprehensive screening of some west and central African sesame genotypes for drought resistance probing by agromorphological, physiological, biochemical and seed quality traits. Agronomy. 7(4):83. https://doi.org/10.3390/agronomy7040083

    Article  CAS  Google Scholar 

  33. Ahmed Z, Waraich EA, Ahmad R, Shahbaz M (2017) Morpho-physiological and biochemical responses of camelina (Camelina sativa crantz) genotypes under drought stress. Int J Agric Biol 19:1–7. https://doi.org/10.17957/IJAB/15.0141

    Article  Google Scholar 

  34. Waraich EA, Rashid F, Ahmad Z, Ahmad R, Ahmad M (2020) Foliar applied potassium stimulate drought tolerance in canola under water deficit conditions. J Plant Nutr 43:1–12. https://doi.org/10.1080/01904167.2020.1758132

    Article  CAS  Google Scholar 

  35. Gao L, Caldwell CD, Jiang Y (2018) Photosynthesis and growth of camelina and canola in response to water deficit and applied nitrogen. Crop Sci 58(1):393–401. https://doi.org/10.2135/cropsci2017.07.0406

    Article  CAS  Google Scholar 

  36. Ahmad M, El-Saeid MH, Akram MA et al (2016) Silicon fertilization – a tool to boost up drought tolerance in wheat (Triticum aestivum L.) crop for better yield. J Plant Nutr 39:1283–1291. https://doi.org/10.1080/01904167.2015.1105262

    Article  CAS  Google Scholar 

  37. Urbaniak SD, Caldwell CD, Zheljazkov VD, Lada R, Luan L (2008) The effect of cultivar and applied nitrogen on the performance of Camelina sativa L. in the maritime provinces of Canada. Can J Plant Sci 88(1):111–119. https://doi.org/10.4141/CJPS07115

    Article  CAS  Google Scholar 

  38. Jaleel CA, Manivannan P, Wahid A et al (2009) Drought stress in plants: a review on morphological characteristics and pigments composition. Int J Agric Biol 11(1):100–105

    Google Scholar 

  39. Shehzad MA, Nawaz F, Ahmad F, Ahmad N, Masood S (2020) Protective effect of potassium and chitosan supply on growth, physiological processes and antioxidative machinery in sunflower (Helianthus annuus L.) under drought stress. Ecotoxicol Environ Saf 187:109841. https://doi.org/10.1016/j.ecoenv.2019.109841

    Article  CAS  PubMed  Google Scholar 

  40. El Sabagh A, Hossain A, Barutçular C et al (2019) Effects of drought stress on the quality of major oilseed crops: implications and possible mitigation strategies - a review. Appl Ecol Environ Res 17:4019–4043. https://doi.org/10.15666/aeer/1702_40194043

    Article  Google Scholar 

  41. Choudhury FK, Rivero RM, Blumwald E, Mittler R (2017) Reactive oxygen species, abiotic stress and stress combination. Plant J 90(5):856–867. https://doi.org/10.1111/tpj.13299

    Article  CAS  PubMed  Google Scholar 

  42. Ullah A, Sun H, Yang X, Zhang X (2017) Drought coping strategies in cotton: increased crop per drop. Plant Biotechnol J 15(3):271–284

    Article  CAS  Google Scholar 

  43. Osmolovskaya N, Shumilina J, Kim A, Didio A, Grishina T, Bilova T, Keltsieva OA, Zhukov V, Tikhonovich I, Tarakhovskaya E, Frolov A, Wessjohann LA (2018) Methodology of drought stress research: experimental setup and physiological characterization. Int J Mol Sci 19(12):4089. https://doi.org/10.3390/ijms19124089

    Article  PubMed Central  Google Scholar 

  44. Laxa M, Liebthal M, Telman W, Chibani K, Dietz KJ (2019) The role of the plant antioxidant system in drought tolerance. Antioxidants. 8(4):94. https://doi.org/10.3390/antiox8040094

    Article  CAS  PubMed Central  Google Scholar 

  45. Hussain M, Farooq S, Hasan W, Ul-Allah S, Tanveer M, Farooq M, Nawaz A (2018) Drought stress in sunflower: physiological effects and its management through breeding and agronomic alternatives. Agric Water Manag 201:152–166. https://doi.org/10.1016/j.agwat.2018.01.028

    Article  Google Scholar 

  46. Wang P, Yang C, Chen H, Song C, Zhang X, Wang D (2017) Transcriptomic basis for drought-resistance in Brassica napus L. Sci Rep 7(1):1–20. https://doi.org/10.1038/srep40532

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Agronomy, Faculty of Agriculture and Environmental Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan

    Muhammad Adnan Bukhari & Muhammad Shahzad Sharif

  2. Department of Field Crops, Faculty of Agriculture, Çukurova University, 1380, Adana, Turkey

    Zahoor Ahmad & Celaleddin Barutçular

  3. College of Food and Agriculture Sciences, Plant Production Department, King Saud University, Riyadh, Saudi Arabia

    Muhammad Afzal

  4. Bangladesh Wheat and Maize Research Institute, Dinajpur, Rājshāhi, 5200, Bangladesh

    Akbar Hossain

  5. Department of Field crops, Faculty of Agriculture, Siirt University, 56100, Siirt, Turkey

    Ayman EL Sabagh

  6. Department of Agronomy, Faculty of Agriculture, University of Kafrelsheikh, Kafrelsheikh, 33516, Egypt

    Ayman EL Sabagh

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  1. Muhammad Adnan Bukhari
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  2. Muhammad Shahzad Sharif
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  3. Zahoor Ahmad
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Contributions

MAD plan and supervise the research and MS conduct research work in wire-house; MA and ZA write the introduction part; MAD and MS write the manuscript; ZA help to statical analysis and graphical representation; CB read the manuscript as proofreading; AH and AE help in English editing and final formatting according to journal style.

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Correspondence to Zahoor Ahmad.

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Bukhari, M.A., Sharif, M.S., Ahmad, Z. et al. Silicon Mitigates the Adverse Effect of Drought in Canola (Brassica napus l.) Through Promoting the Physiological and Antioxidants Activity. Silicon 13, 3817–3826 (2021). https://doi.org/10.1007/s12633-020-00685-x

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