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Identification of Functional Genetic Polymorphisms at IL-10 Promoter Region and their Association with Risk of Ischemic Stroke in Chinese Han Population

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The journal of nutrition, health & aging

Abstract

Background

Pleiotropic cytokine interleukin-10 (IL-10) has been suggested as modifying risk for atherosclerosis. Promoter region genetic polymorphism of IL-10 gene is known to be associated with the level of IL-10 production. Through these effects, they might be involved with the ischemic stroke (IS). Our aim was to assess the nature of the functional polymorphisms in IL-10 promoter and any links with IS in Chinese Han population.

Methods

The IL-10 -1082 G>A, -819 C>T and -592 C>A functional polymorphisms were determined by TaqMan SNP Genotyping assays in 1296 subjects (648 IS patients diagnosed by CTs or/and magnetic resonance imaging (MRI) and 648 normal healthy controls from Chinese Han Population). Multivariate logistic regression analysis was used to show the association between the IL-10 genotypes and the IS events.

Results

We found that the CC genotype of -819 C>T was more common in controls than IS subjects (P<0.05, corrected for multiple testing) in the Han population. CC carriage may be associated with the decreased risk of IS in the Han ethnic group (OR 0.65, 95% CI 0.43-0.97). In addition, the CC genotype in -592 C>A and GG genotype in -1082 G>A are all extremely low in Chinese Han cases and controls.

Conclusions

The G allele frequency at the -1082 promoter region of IL-10 was rare in Chinese Han population which are diffierent from American/European populations.. IL-10 -819 C>T may be an independent protective factor for IS in the Chinese Han population.

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Author notes

  1. Yeqing Tong, Shunli Jiang, Li Cai,Xuhua Guan, Shuangyi Hou and Zhihong Wang contributed equally as the first authors.

  2. Jiafa Liu and Qing Lu contributed equally as the corresponding authors

Authors and Affiliations

  1. Center for Disease Control and Prevention, Hubei, 430079, China

    Yeqing Tong, Xuhua Guan, Shuangyi Hou, Zhihong Wang & Jiafa Liu

  2. School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Yeqing Tong, Shunli Jiang & Qing Lu

  3. Wuhan Center for Disease Control and Prevention, Wuhan, 430015, P. R. China

    Li Cai

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  1. Yeqing Tong
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Correspondence to Qing Lu or Jiafa Liu.

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Tong, Y., Jiang, S., Cai, L. et al. Identification of Functional Genetic Polymorphisms at IL-10 Promoter Region and their Association with Risk of Ischemic Stroke in Chinese Han Population. J Nutr Health Aging 22, 779–784 (2018). https://doi.org/10.1007/s12603-018-1012-x

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