The journal of nutrition, health & aging

, Volume 21, Issue 3, pp 233–240 | Cite as

Consumption of nuts and seeds and telomere length in 5,582 men and women of the National Health and Nutrition Examination Survey (NHANES)




Consumption of nuts and seeds is associated favorably with all-cause mortality. Nuts and seeds could reduce disease and prolong life by influencing telomeres. Telomere length is a good indicator of the senescence of cells. The purpose of the present study was to determine the relationship between nuts and seeds intake and leukocyte telomere length, a biomarker of biologic aging.



Setting and Participants

A total of 5,582 randomly selected men and women from the National Health and Nutrition Examination Survey (NHANES), 1999-2002, were studied.


DNA was obtained via blood samples. Telomere length was assessed using the quantitative polymerase chain reaction method. A validated, multi-pass, 24-h recall dietary assessment, administered by NHANES, was employed to quantify consumption of nuts and seeds.


Nuts and seeds intake was positively and linearly associated with telomere length. For each 1-percent of total energy derived from nuts and seeds, telomere length was 5 base pairs longer (F=8.6, P=0.0065). Given the age-related rate of telomere shortening was 15.4 base pairs per year (F=581.1, P<0.0001), adults of the same age had more than 1.5 years of reduced cell aging if they consumed 5% of their total energy from nuts and seeds.


Consumption of nuts and seeds accounts for meaningful decreases in biologic aging and cell senescence. The findings reinforce the recommendations of the 2015-2020 Dietary Guidelines for Americans, which encourage the consumption of nuts and seeds as part of a healthy diet.

Key words

Cell aging nuts DNA dietary fat NHANES 


  1. 1.
    U.S. Department of Health and Human Services and the U.S. Department of Agriculture. 2015-2020 Dietary Guidelines for Americans. 8th Edition ed.; 2015.Google Scholar
  2. 2.
    Guasch-Ferre, M.; Bullo, M.; Martinez-Gonzalez, M.A.; Ros, E.; Corella, D.; Estruch, R.; Fito, M.; Aros, F.; Warnberg, J.; Fiol, M.; Lapetra, J.; Vinyoles, E.; Lamuela-Raventos, R.M.; Serra-Majem, L.; Pinto, X.; Ruiz-Gutierrez, V.; Basora, J.; Salas-Salvado, J.; Frequency of nut consumption and mortality risk in the PREDIMED nutrition intervention trial. BMC Med 2013, 11, 164.CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Ellsworth, J.L.; Kushi, L.H.; Folsom, A.R. Frequent nut intake and risk of death from coronary heart disease and all causes in postmenopausal women: the Iowa Women’s Health Study. Nutr Metab Cardiovasc Dis 2001, 11, 372–377.PubMedGoogle Scholar
  4. 4.
    Hshieh, T.T.; Petrone, A.B.; Gaziano, J.M.; Djousse, L. Nut consumption and risk of mortality in the Physicians’ Health Study. Am J Clin Nutr 2015, 101, 407–412.CrossRefPubMedGoogle Scholar
  5. 5.
    Sabate, J.; Oda, K.; Ros, E. Nut consumption and blood lipid levels: a pooled analysis of 25 intervention trials. Arch Intern Med 2010, 170, 821–827.CrossRefPubMedGoogle Scholar
  6. 6.
    Albert, C.M.; Gaziano, J.M.; Willett, W.C.; Manson, J.E. Nut consumption and decreased risk of sudden cardiac death in the Physicians’ Health Study. Arch Intern Med 2002, 162, 1382–1387.CrossRefPubMedGoogle Scholar
  7. 7.
    Ros, E. Nuts and novel biomarkers of cardiovascular disease. Am J Clin Nutr 2009, 89, 1649S–1656S.CrossRefPubMedGoogle Scholar
  8. 8.
    Ros, E. Health benefits of nut consumption. Nutrients 2010, 2, 652–682.CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Kris-Etherton, P.M.; Hu, F.B.; Ros, E.; Sabate, J. The role of tree nuts and peanuts in the prevention of coronary heart disease: multiple potential mechanisms. J Nutr 2008, 138, 1746S–1751S.PubMedGoogle Scholar
  10. 10.
    Tapsell, L.C.; Neale, E.P.; Satija, A.; Hu, F.B. Foods, Nutrients, and Dietary Patterns: Interconnections and Implications for Dietary Guidelines. Adv Nutr 2016, 7, 445–454.CrossRefPubMedGoogle Scholar
  11. 11.
    Curtin, L.R.; Mohadjer, L.K.; Dohrmann, S.M.; Montaquila, J.M.; Kruszan-Moran, D.; Mirel, L.B.; Carroll, M.D.; Hirsch, R.; Schober, S.; Johnson, C.L. The National Health and Nutrition Examination Survey: Sample Design, 1999-2006. Vital Health Stat 2 2012, 1–39.Google Scholar
  12. 12.
    Centers for Disease Control and Prevention. National Health and Nutrition Examination Survey Laboratory Protocol, Data Documentation, Codebook, and Frequencies: 2001-2002. Available online: Accessed on 1 September 2016.Google Scholar
  13. 13.
    Needham, B.L.; Adler, N.; Gregorich, S.; Rehkopf, D.; Lin, J.; Blackburn, E.H.; Epel, E.S. Socioeconomic status, health behavior, and leukocyte telomere length in the National Health and Nutrition Examination Survey, 1999-2002. Soc Sci Med 2013, 85, 1–8.CrossRefPubMedPubMedCentralGoogle Scholar
  14. 14.
    Cawthon, R.M. Telomere measurement by quantitative PCR. Nucleic Acids Res 2002, 30, e47.CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Centers for Disease Control and Prevention. NHANES. MEC in-person dietary interviewers procedures manual. U.S. Department of Health and Human Services, Center for Disease Control and Prevention: 2002. Available online: Accessed on 1 September 2016).Google Scholar
  16. 16.
    Haddad, E.H.; Tanzman, J.S. What do vegetarians in the United States eat? Am J Clin Nutr 2003, 78, 626S–632S.PubMedGoogle Scholar
  17. 17.
    Farmer, B.; Larson, B.T.; Fulgoni, V.L., 3rd; Rainville, A.J.; Liepa, G.U. A vegetarian dietary pattern as a nutrient-dense approach to weight management: an analysis of the national health and nutrition examination survey 1999-2004. J Am Diet Assoc 2011, 111, 819–827.CrossRefPubMedGoogle Scholar
  18. 18.
    Kant, A.K.; Graubard, B.I.; Mattes, R.D. Association of food form with self-reported 24-h energy intake and meal patterns in US adults: NHANES 2003-2008. Am J Clin Nutr 2012, 96, 1369–1378.CrossRefPubMedGoogle Scholar
  19. 19.
    NHANES. Dietary Interview, Individual Foods. Available online: https://wwwn.cdc. gov/Nchs/Nhanes/2001-2002/DRXIFF_B.htm Accessed on 1 September 2016.Google Scholar
  20. 20.
    Ainsworth, B.E.; Haskell, W.L.; Whitt, M.C.; Irwin, M.L.; Swartz, A.M.; Strath, S.J.; O’Brien, W.L.; Bassett, D.R., Jr; Schmitz, K.H.; Emplaincourt, P.O.; Jacobs, D.R., Jr.; Leon, A.S. Compendium of physical activities: an update of activity codes and MET intensities. Med Sci Sports Exerc 2000, 32, S498–504.CrossRefPubMedGoogle Scholar
  21. 21.
    Pescatello, L.S.; American College of Sports Medicine. ACSM’s guidelines for exercise testing and prescription. 9th ed.; Wolters Kluwer/Lippincott Williams & Wilkins Health: Philadelphia, 2014; p xxiv, 456 p.Google Scholar
  22. 22.
    Johnson, C.L.; Paulose-Ram, R.; Ogden, C.L.; Carroll, M.D.; Kruszon-Moran, D.; Dohrmann, S.M.; Curtin, L.R. National health and nutrition examination survey: analytic guidelines, 1999-2010. Vital Health Stat 2 2013, 1–24.Google Scholar
  23. 23.
    Cassidy, A.; De Vivo, I.; Liu, Y.; Han, J.; Prescott, J.; Hunter, D.J.; Rimm, E.B. Associations between diet, lifestyle factors, and telomere length in women. Am J Clin Nutr 2010, 91, 1273–1280.CrossRefPubMedGoogle Scholar
  24. 24.
    Lee, J.Y.; Jun, N.R.; Yoon, D.; Shin, C.; Baik, I. Association between dietary patterns in the remote past and telomere length. Eur J Clin Nutr 2015, 69, 1048–1052.CrossRefPubMedGoogle Scholar
  25. 25.
    Zhou, M.; Zhu, L.; Cui, X.; Feng, L.; Zhao, X.; He, S.; Ping, F.; Li, W.; Li, Y. Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study. Nutr J 2016, 15, 39.CrossRefPubMedPubMedCentralGoogle Scholar
  26. 26.
    Marin, C.; Delgado-Lista, J.; Ramirez, R.; Carracedo, J.; Caballero, J.; Martinez, P.; Gutierrez-Mariscal, F.M.; Garcia-Rios, A.; Delgado-Casado, N.; Cruz-Teno, C.; Yubero-Serrano, E.M.; Tinahones, F.; Malagon Mdel, M.; Perez-Jimenez, F.; Lopez-Miranda, J. Mediterranean diet reduces senescence-associated stress in endothelial cells. Age (Dordr) 2012, 34, 1309–1316.CrossRefGoogle Scholar
  27. 27.
    Boccardi, V.; Esposito, A.; Rizzo, M.R.; Marfella, R.; Barbieri, M.; Paolisso, G. Mediterranean diet, telomere maintenance and health status among elderly. PLoS One 2013, 8, e62781.CrossRefPubMedPubMedCentralGoogle Scholar
  28. 28.
    Crous-Bou, M.; Fung, T.T.; Prescott, J.; Julin, B.; Du, M.; Sun, Q.; Rexrode, K.M.; Hu, F.B.; De Vivo, I. Mediterranean diet and telomere length in Nurses’ Health Study: population based cohort study. BMJ 2014, 349, g6674.Google Scholar
  29. 29.
    Gu, Y.; Honig, L.S.; Schupf, N.; Lee, J.H.; Luchsinger, J.A.; Stern, Y.; Scarmeas, N. Mediterranean diet and leukocyte telomere length in a multi-ethnic elderly population. Age (Dordr) 2015, 37, 24.CrossRefGoogle Scholar
  30. 30.
    von Zglinicki, T. Oxidative stress shortens telomeres. Trends Biochem Sci 2002, 27, 339–344.CrossRefGoogle Scholar
  31. 31.
    Houben, J.M.; Moonen, H.J.; van Schooten, F.J.; Hageman, G.J. Telomere length assessment: biomarker of chronic oxidative stress? Free Radic Biol Med 2008, 44, 235–246.CrossRefPubMedGoogle Scholar
  32. 32.
    Jiang, R.; Jacobs, D.R., Jr.; Mayer-Davis, E.; Szklo, M.; Herrington, D.; Jenny, N.S.; Kronmal, R.; Barr, R.G. Nut and seed consumption and inflammatory markers in the multi-ethnic study of atherosclerosis. Am J Epidemiol 2006, 163, 222–231.CrossRefPubMedGoogle Scholar
  33. 33.
    Jenkins, D.J.; Kendall, C.W.; Marchie, A.; Parker, T.L.; Connelly, P.W.; Qian, W.; Haight, J.S.; Faulkner, D.; Vidgen, E.; Lapsley, K.G.; Spiller, G.A. Dose response of almonds on coronary heart disease risk factors: blood lipids, oxidized low-density lipoproteins, lipoprotein(a), homocysteine, and pulmonary nitric oxide: a randomized, controlled, crossover trial. Circulation 2002, 106, 1327–1332.CrossRefPubMedGoogle Scholar
  34. 34.
    Casas-Agustench, P.; Lopez-Uriarte, P.; Bullo, M.; Ros, E.; Cabre-Vila, J.J.; Salas-Salvado, J. Effects of one serving of mixed nuts on serum lipids, insulin resistance and inflammatory markers in patients with the metabolic syndrome. Nutr Metab Cardiovasc Dis 2011, 21, 126–135.CrossRefPubMedGoogle Scholar
  35. 35.
    Salas-Salvado, J.; Casas-Agustench, P.; Murphy, M.M.; Lopez-Uriarte, P.; Bullo, M. The effect of nuts on inflammation. Asia Pac J Clin Nutr 2008, 17 Suppl 1, 333–336.PubMedGoogle Scholar
  36. 36.
    Casas-Agustench, P.; Bullo, M.; Salas-Salvado, J. Nuts, inflammation and insulin resistance. Asia Pac J Clin Nutr 2010, 19, 124–130.PubMedGoogle Scholar

Copyright information

© Serdi and Springer-Verlag France 2017

Authors and Affiliations

  1. 1.College of Life SciencesBrigham Young UniversityProvoUSA

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