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Biomarkers of oxidative stress in patients with wet age related macular degeneration



The aim of this study was to analyze biomarkers of oxidative stress in patients with wet age related macular degeneration (AMD).

Participants and Measurements

Case-control study that includes 163 patients with wet AMD (age group of 55–82 years with the mean age of 71 years and 170 age-matched healthy controls in the age group of 55–78 years with the mean age of 71 years. The following parameters were determined: reduced and oxidized Glutathione (GSH/GSSH), protein carbonyl groups, total antioxidant activity in plasma and the activity of endogenous antioxidant enzymes, such as, gluthatione peroxidase, gluthatione reductase and superoxide dismutase.


We observed total antioxidant activity higher in control group (CG) compared with patients with wet AMD (7.1 ± 1.2 μM Trolox vs 5.8 ± 1.1 μM Trolox). Values of superoxide dismutase activity (SOD), gluthatione reductase (GR) and gluthatione peroxidase (GPx) are higher in control group than in patients with wet AMD. According to the GSH/GSSH results, average values were higher in the CG than in patients with wet AMD and data were not significantly different.. Values of protein carbonyl groups were higher in patients with wet AMD than in CG and significant differences were found.


The finding of the present study suggests that the patients with wet AMD are an altered metabolic state of oxidation-reduction and that it is convenient to give therapeutic interventions with antioxidants. We have demonstrated that systematic oxidative stress, measured by different biomarkers is closely associated with the wet AMD.

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  1. Klein R, Chou CF, Klein BE, Zhang X, Meuer SM, Saaddine JB (2011) Prevalence of age-related macular degeneration in the U.S. population. Arch Ophthalmol 129: 75–80.

    PubMed  Article  Google Scholar 

  2. Haddad S, Chen CA, Santangelo S, Seddon JM (2006) The genetics of age-related macular degeneration: A review of progress to date. Survey of Ophthalmology 51: 316–363.

    PubMed  Article  Google Scholar 

  3. Beatty S, Koh H, Phil M, Henson D, Boulton M (2000) The role of oxidative stress in the pathogenesis of age-related macular degeneration. Surv Ophthalmol 45:115–34.

    PubMed  Article  CAS  Google Scholar 

  4. Drobek-Słowik M, Karczewicz D, Safranow K (2007) The potential role of oxidative stress in the pathogenesis of the age-related macular degeneration (AMD). Postepy Hig Med Dosw 61:28–37.

    Google Scholar 

  5. Babizhayez MA, Burke L, Micans P, Richer SP (2009) N-acteylcarnosine sustained drug delivery eye drops to control the signs of ageless vision: Glare sensitivity, cataract amelioration and quality of vision currently available treatment for the challenging 50,000 patient population. Clin Interv Aging 4:31–50.

    Google Scholar 

  6. Brubaker RF, Bourne WM, Bachman LA, McLaren JW (2000) Ascorbic acid content of human corneal epithelium. Invest Ophthalmol Vis Sci 41:1681–3.

    PubMed  CAS  Google Scholar 

  7. Dávalos A, Gómez-Cordovés C, Bartolomé B (2004) Extending applicability of the oxygen radical absorbance capacity (ORAC-Fluorescein) assay. J. Agric. Food Chem 52: 48–54.

    PubMed  Article  Google Scholar 

  8. Plagia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte gluthatione peroxidase J. Lab Clin Med 70: 158–169.

    Google Scholar 

  9. Anderson JW, Foyer CH, Walker DA (1983) Reduction of dehydro-ascorbate and uptake of exogenous ascorbate by spinach chloroplasts. Planta 158: 442–450.

    Article  CAS  Google Scholar 

  10. Delmas-Beauvieux MC, Peuchant E, Couchouron A (1996) The enzymatic antioxidant system in blood and glutathione status in human immunodeficiency virus (HIV)-infected patients: effects of supplementation with selenium or β-carotene Am. J. Clin Nutr 64: 101–107.

    PubMed  CAS  Google Scholar 

  11. Cai J, Nelson KC, Wu M, Sternberg P, Jones DP (2000) Oxidative damage and protection of the RPE. Prog Retin Eye Res 19: 205–221.

    PubMed  Article  CAS  Google Scholar 

  12. Uno K, Nicholls SJ (2010) Biomarkers of inflammation and oxidative stress in atherosclerosis. Biomark Med 4: 361–73.

    PubMed  Article  CAS  Google Scholar 

  13. Likidlilid A, Patchanans N, Peerapatdit T, Sriratanasathavorn C (2010) Lipid peroxidation and antioxidant enzyme activities in erythrocytes of type 2 diabetic patients. J Med Assoc Thai 93: 682–693.

    PubMed  Google Scholar 

  14. Shen XL, Jia JH, Zhao P, Fan R, Pan XY, Yang HM, Liu L (2012) Changes in blood oxidative and antioxidant parameters in a group of Chinese patients with age-related macular degeneration. Nutr Health Aging 16:201–204.

    Article  CAS  Google Scholar 

  15. Cohen SM, Olin KL, Feuer WJ, Hjelmeland L, Keen CL, Morse LS (1994) Low glutathione reductase and peroxidase activity in age-related macular degeneration Br J Ophthalmol 78: 791–794.

    PubMed  Article  CAS  Google Scholar 

  16. Popovici I, Darabă O, Petrariu FD (1994) Age related macular degeneration: biochemical aspects the journal of preventive medicine 12: 22–29.

    Google Scholar 

  17. Siess, H (1991) Oxidative stress: from Basic Research to Clinical Application. Am. J. Med 91: 38–85.

    Article  Google Scholar 

  18. Justilien V, Pang JJ, Renganathan K, et al. SOD2 (2007) Knockdown mouse model of early AMD. Invest Ophthalmol Vis Sci 48, 4407–20.

    PubMed  Article  Google Scholar 

  19. Hashizume K, Hirasawa M, Imamura Y, et al. Retinal dysfunction and progressive retinal cells death in SOD1-Deficient mice. Am J Pathol 2008; 172(5): 1325–31.

    PubMed  Article  Google Scholar 

  20. Evereklioglu C, Er H, Doganay S, Cekmen M, Turkoz Y, Otlu B, Ozerol E (2003) Nitric oxide and lipid peroxidation are increased and associated with decreased antioxidant enzyme activities in patients with age-related macular degeneration. Doc Ophthalmol 106: 129–136.

    PubMed  Article  Google Scholar 

  21. Jia L, Dong Y, Yang H, Pan X, Fan R, Zhai L (2011) Serum superoxide dismutase and malondialdehyde levels in a group of Chinese patients with age-related macular degeneration. Aging Clin Exp Res 23:264–267.

    PubMed  CAS  Google Scholar 

  22. Prashar S, Pandav SS, Gupta A, Nath R (1993) Antioxidant enzymes in RBCs as a biological index of age related macular degeneration. Acta Ophthalmol 71: 214–218.

    CAS  Google Scholar 

  23. Anderson ME (1998) Glutathione: an overview of biosynthesis and modulation. Chem Biol Interact 111:1–14.

    PubMed  Article  Google Scholar 

  24. Julius M, Lang CA, Gliebermant L, DiFranceisco W, Schork A (1994) Glutathione and morbidity in a community based sample of elderly. J Clin Epidemiol 47:1021–1026.

    PubMed  Article  CAS  Google Scholar 

  25. Samiec PS, Drews-Botsch C, Flagg EW, Kurtz JC, Sternberg P Jr, Reed RL, Jones DP (1998) Glutathione in human plasma: decline in association with aging, age-related macular degeneration, and diabetes. Free Radic Biol Med 24: 699–704.

    PubMed  Article  CAS  Google Scholar 

  26. Davies MJ, Fu S, Wang H & Dean RT (1999) Stable markers of oxidant damage to proteins and their application in the study of human disease. Free Radic Biol Med 27, 1151–1163.

    PubMed  Article  CAS  Google Scholar 

  27. Venza I, Visalli M, Cucinotta M, Teti D, Venza M (2012) Association between oxidative stress and macromolecular damage in elderly patients with age-related macular degeneration. Aging Clin Exp Res 24:21–27.

    PubMed  CAS  Google Scholar 

  28. Serap, Y, Rezan H,, Koray B, Mehmet B, Ülkü C, Pelin E (2011) Antioxidant statues in senile macular degeneration. Acta Physiologica 203, Sup 686.

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Correspondence to P. Zafrilla.

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Zafrilla, P., Losada, M., Perez, A. et al. Biomarkers of oxidative stress in patients with wet age related macular degeneration. J Nutr Health Aging 17, 219–222 (2013).

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Key words

  • Protein carbonyl groups
  • age related macular degeneration (ARMD)
  • antioxidant enzymes