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Effects of margarine supplemented with T10C12 and C9T11 CLA on atherosclerosis and steatosis in apoE/LDLR -/- mice

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The journal of nutrition, health & aging

Abstract

Objectives

The objective of this study was to evaluate functional effects of margarine supplemented with individual CLA isomers trans-10, cis-12 and cis-9, trans-11 in apoE/LDLR -/- mice.

Design

In LONG experiment (LONG), two-month old mice with no atherosclerosis were assigned to experimental groups and fed for the next 4 months. In SHORT experiment (SHORT), four-month old mice, with pre-established atherosclerosis, were assigned to experimental groups and fed for the next 2 months. The experimental diets were: ALN-93G (margarine), AIN-93G + 0.5% trans-10, cis-12 CLA (tl0cl2), and AIN-93G + 0.5% cis-9, trans-11 CLA (c9tll).

Results

In both experiments (LONG and SHORT), liver weight was significantly (P<0.05) increased in mice fed t110c12 CLA. Hepatic steatosis was found in animals fed t110c12 diet and no signs of the steatosis was observed in mice fed c9tll CLA. Dietary treatments with t110c12 CLA significantly increased total plasma cholesterol and plasma triacylglycerols. There were no isomer-specific effects of CLA isomers on area of atherosclerotic plaque in aortic root.

Conclusion

In conclusion, t110c12 CLA significantly increased liver weight in mice in LONG and SHORT experiments. Our results do not support the notion that CLA isomer supplementation to the margarine possess anti-atheroclerotic effect. Therefore, no isomer-specific effects of CLA on development of atherosclerosis were observed.

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References

  1. Evans M, Brown J, Mcintosh M (2002) Isomer-specific effects of conjugated linoleic acid (CLA) on adiposity and lipid metabolism. J Nutr Biochem 13: 508–516

    Article  PubMed  CAS  Google Scholar 

  2. Wang Y, Jones PJH (2004) The role of conjugated linoleic acid in human health. Dietary conjugated linoleic acid and body composition. Am J Clin Nutr 79: 1153S–1158S

    PubMed  CAS  Google Scholar 

  3. Belury MA (2002) Inhibition of carcinogenesis by conjugated linoleic acid: potential mechanisms of action. J Nutr 132:2995–2998

    PubMed  CAS  Google Scholar 

  4. Field CJ, Schley PD (2004) Evidence for potential mechanisms for the effect of conjugated linoleic acid on tumor metabolism and immune function: lessons from n-3 fatty acids. Am J Clin Nutr 79:1190S–1198S

    PubMed  CAS  Google Scholar 

  5. Kritchevsky D, Tepper SA, Wright S et al. (2002) Influence of graded levels of conjugated linoleic acid (CLA) on experimental atherosclerosis in rabbits. Nutrition Research 22: 1275–1279

    Article  CAS  Google Scholar 

  6. Arbonés-Mainar JM, Navarro MA, Acín S et al. (2006) Trans-10, cis-12- and cis-9, trans-11-Conjugated Linoleic Acid Isomers Selectively Modify HDL-Apolipoprotein Composition in Apolipoprotein E Knockout Mice. J Nutr 136: 353–359

    PubMed  Google Scholar 

  7. Toomey S, Harhen B, Roche HM et al. (2006) Profound resolution of early atherosclerosis with conjugated linoleic acid. Atherosclerosis 187: 40–49

    Article  PubMed  CAS  Google Scholar 

  8. Franczyk-Żarów M, Kostogrys RB, Szymczyk B et al. (2008) Functional effects of eggs, naturally enriched with conjugated linoleic acid (CLA), on the blood lipid profile, development of atherosclerosis and composition of atherosclerotic plaque in apolipoprotein E and low density lipoprotein receptor double-knockout mice (apoE/LDLR -/-). Brit J Nutr 99:49–58

    PubMed  Google Scholar 

  9. O’shea M, Bassaganya-Riera J, Mohede ICM (2004) Immunomodulatory properties of conjugated linoleic acid. Am J Clin Nutr 79:1199S–1206S

    PubMed  Google Scholar 

  10. Azain MJ (2003) Conjugated linoleic acid and its effects on animal products and health in single stomached animals. Proc Nutr Soc 62: 319–328

    Article  PubMed  CAS  Google Scholar 

  11. Raes K, De Smet S, Demeyer D (2004) Effects of dietary fatty acids on incorporation of long chain polyunsaturated fatty acids and conjugated linoleic acid in lamb, beef and pork meat. Anim Feed Sci Tech 113: 199–221

    Article  CAS  Google Scholar 

  12. Givens DI (2005) The role of animal nutrition in improving the nutritive value of animal-derived foods in relation to chronic disease. Proc Nutr Soc 64: 395–402

    Article  PubMed  CAS  Google Scholar 

  13. Pisulewski PM, Franczyk M, Kostogrys RB (2005) Heath-related effects of nutritionally modified foods of animal origin. J Anim Feed Sci 14:71S–85S

    Google Scholar 

  14. Schmid A, Collomb M, Sieber R et al. (2006) Conjugated linoleic acid in meat and meat products: A review. Meat Sci 73:29–41

    Article  PubMed  CAS  Google Scholar 

  15. Jawien J, Gajda M, Rudling M et al. (2006a) Inhibition of five lipoxygenase activating protein (FLAP) by MK-886 decreases atherosclerosis in apoE/LDLR-double knockout mice. Eur J Clin Invest 36:141–146

    Article  PubMed  CAS  Google Scholar 

  16. Olszanecki R, Jawien J, Gajda M et al. (2005) Effect of curcumin on atherosclerosis in apoE/LDLR-double knockout mice. J Physiol Pharmacol 56: 627–635

    PubMed  CAS  Google Scholar 

  17. Jawien J, Csanyi G, Gajda M et al. (2006b) Ticlopidine attenuates progression of atherosclerosis in apolipoprotein E and low density lipoprotein receptor double knockout mice. Eur J Pharmacol 556:129–35

    Article  PubMed  Google Scholar 

  18. Ishibashi S, Herz J, Maeda N et al. (1994) The two-receptor model of lipoprotein clearance: tests of the hypothesis in “knockout” mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins. Proc Natl Acad Sci USA 91:4431–4435

    Article  PubMed  CAS  Google Scholar 

  19. Reeves PG, Nielsen FH, Fahey GC (1993). AIN-93 purified diets for laboratory rodents: Final report of the American Institute of Nutrition ad hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951

    PubMed  CAS  Google Scholar 

  20. Robertson AK, Rudling M, Zhou X et al. (2003) Disruption of TGF-beta signaling in T cells accelerates atherosclerosis. J Clin Invest 112: 1342–1350

    PubMed  CAS  Google Scholar 

  21. Kieman JA (1990) Histological & Histochemical Methods. Theory & Practice, 2 nd edition. Oxfort: Pergamon Press, Great Britain BPCC, Wheatons LTD, Exeter, pp 413–421

    Google Scholar 

  22. Ryder JW, Portocarrero CP, Song XM, et al. (2001) Isomer-specific antidiabetic properties of conjugated linoleic acid. Improved glucose tolerance, skeletal muscle insulin action, and UCP-2 gene expression. Diabetes 50: 1149–1157

    Article  PubMed  CAS  Google Scholar 

  23. Park Y, Albright KJ, Liu W et al. (1997) Effect of conjugated linoleic acid on body composition in mice. Lipids 32: 853–858

    Article  PubMed  CAS  Google Scholar 

  24. Park Y, Albright KJ, Storkson JM et al. (1999) Changes in body composition in mice during feeding and withdrawal of conjugated linoleic acid. Lipids 34: 243–248

    Article  PubMed  CAS  Google Scholar 

  25. West DB, Delany JP, Carnet PM et al. (1998) Effects of conjugated linoleic acid on body fat and energy metabolism in the mouse. Am J Physiol 275: R667–672

    PubMed  CAS  Google Scholar 

  26. Park Y, Pariza MW (2007) Mechanisms of body fat modulation by conjugated linoleic acid (CLA). Food Res Int, 40: 311–323

    Article  CAS  Google Scholar 

  27. Park Y (2009) Conjugated linoleic acid (CLA): Good or bad trans fat? J Food Com Anal 22S: S4–S12

    Article  Google Scholar 

  28. Warren JM, Simon VA, Bartolini G et al. (2003) Trans-10,cis-12 CLA increases liver and decreases adipose tissue lipids in mice: possible roles of specific lipid metabolism genes. Lipids 38: 497–504

    Article  PubMed  CAS  Google Scholar 

  29. Ashwell MS, Ceddia RP, House RL et al. (2010) Trans-10, cis-12-conjugated linoleic acid alters hepatic gene expression in a polygenic obese line of mice displaying hepatic lipidosis. J Nutr Biochem 21:9, 848–855

    Article  PubMed  CAS  Google Scholar 

  30. Tsuboyama-Kasaoka N, Takahashi M, Tanemura K, et al. (2000) Conjugated linoleic acid supplementation reduces adipose tissue by apoptosis and develops lipodystrophy in mice. Diabetes 49: 1534–1542

    Article  PubMed  CAS  Google Scholar 

  31. Clement L, Poirier H, Niot I, et al. (2002) Dietary trans-10,cis-12 conjugated linoleic acid induces hyperinsulinemia and fatty liver in the mouse. J Lipid Res 43: 1400–1409

    Article  PubMed  CAS  Google Scholar 

  32. Degrace P, Demizieux L, Gresti J et al. (2004) Hepatic steatosis is not due to impaired fatty acid oxidation capacities in C57BL/6J mice fed the conjugated trans-10,cis-12-isomer of linoleic acid. J Nutr 134: 861–867

    PubMed  CAS  Google Scholar 

  33. Belury MA, Mahon A, Banni S (2003) The conjugated linoleic acid (CLA) isomer, tl0cl2-CLA, is inversely associated with changes in body weight and serum leptin in subjects with type 2 diabetes mellitus. J Nutr 133: 257S–260S

    PubMed  Google Scholar 

  34. Burdge GC, Lupoli B, Russell JJ et al. (2004) Incorporation of cis-9,trans-11 or trans-10,cis-12 conjugated linoleic acid into plasma and cellular lipids in healthy men. J Lipid Res 45: 736–741

    Article  PubMed  CAS  Google Scholar 

  35. Kostogrys RB, Pisulewski PM (2010) Conjugated linoleic acid decreased serum triacyloglycerol and changed fatty acid composition in rat’s liver. J Anim Feed Sci 19: 484–494

    Google Scholar 

  36. Kostogrys RB, Pisulewski PM (2010) Effect of conjugated linoleic acid (CLA) on lipid profile and liver histology in laboratory rats fed high-fructose diet. Environ Toxicol Pharmacol 30: 245–250

    Article  PubMed  CAS  Google Scholar 

  37. Chouinard P, Courneau L, Barbano D et al. (1999) Conjugated linoleic acids alter milk fatty acid composition and inhibit milk fat secretion in dairy cows. J Nutr 129: 1579–1584

    PubMed  CAS  Google Scholar 

  38. Simon O, Manner K, Schafer K et al. (2000) Effects of conjugated linoleic acids on protein to fat proportions, fatty acids, and plasma lipids in broilers. Eur J Lipid Sci Technol 102:402–410

    Article  CAS  Google Scholar 

  39. Szymczyk B, Pisulewski PM, Szczurek W et al. (2000) The effects of feeding conjugated linoleic acid (CLA) on rat growth performance, serum lipoproteins and subsequent lipid composition of selected rat tissues. J Sci Food Agr 80: 1553–1558

    Article  CAS  Google Scholar 

  40. Li Y, Watkins BA (1998) Conjugated linoleic acids alter bone fatty acid composition and reduce ex vivo prostaglandin E2 biosynthesis in rats fed n-6 or n-3 fatty acids. Lipids 33: 417–425

    Article  PubMed  CAS  Google Scholar 

  41. Evans M, Geigerman C, Curtis L et al. (2001) Trans-10, cis-12 conjugated linoleic acid reduces triglyceride content while differentially affecting ppar gamma2 and ap2 expression in 3t3-11 preadipocytes. Lipids 36:1223–1232

    Article  PubMed  CAS  Google Scholar 

  42. Munday JS, Thompson KG, James KAC (1999) Dietary conjugated linoleic acids promote fatty streak formation in the C57BL/6 mouse atherosclerosis model. Br J Nutr 81:251–255

    PubMed  CAS  Google Scholar 

  43. Valeille K, Gripois D, Blouquit MF et al. (2004) Lipid atherogenic risk markers can be more favourably influenced by the cis-9 trans-11-octadecadienoate isomer than a conjugated linoleic acid mixture or fish oil in hamsters. Brit J Nutr 91: 191–199

    Article  PubMed  CAS  Google Scholar 

  44. Tricon S, Burdge GC, Kew S et al. (2004) Opposing effects of cis-9,trans-11 and trans-10,cis-12 conjugated linoleic acid on blood lipids in healthy humans. Am J Clin Nutr 80: 614–620

    PubMed  CAS  Google Scholar 

  45. Yotosumoto H, Hara E, Naka S (1999) 10 trans, 12 cis linoleic acid reduces apolipoprotein B secretion in HepG2 cells. Food Research Int 31:403–409

    Article  Google Scholar 

  46. Grundy SM, Denke MA (1990) Dietary influences on serum lipids and lipoproteins. J Lipid Res 31:1149–1172

    PubMed  CAS  Google Scholar 

  47. Yeung ChH, Yang L, Huang Y (2000) Dietary conjugated linoleic acid mixture affects the activity of intestinal acyl coenzyme A: cholesterol acyltransferase in hamsters. Br J Nutr 84: 935–941

    CAS  Google Scholar 

  48. Fernandez ML, West KL (2005) Mechanisms by which dietary fatty acids modulate plasma lipids. J Nutr 135:2075–2078

    PubMed  CAS  Google Scholar 

  49. Gonzalez FJ (2007) Animal models for human risk assessment: the peroxisome proliferator-activated receptor alpha-humanized mouse. Nutr Rev 65: S2–6

    Article  PubMed  Google Scholar 

  50. Belury MA, Moya-Camarena SY, Liu KL et al. (1997) Dietary conjugated linoleic acid induces peroxisome-specific enzyme accumulation and ornithine decarboxylase activity in mouse liver. J Nutr Biochem 8: 579–584

    Article  CAS  Google Scholar 

  51. Rasooly R, Kelley DS, Greg J et al. (2007) Dietary trans 10, cis 12-conjugated linoleic acid reduces the expression of fatty acid oxidation and drug detoxification enzymes in mouse liver. Brit J Nutr 97: 58–66

    Article  PubMed  CAS  Google Scholar 

  52. Mitchell PL, McLeod RS (2008) Conjugated linoleic acid and atherosclerosis: studies in animal models. Biochem. Cell Biol 86: 293–301

    Article  PubMed  CAS  Google Scholar 

  53. Kostogrys RB, Maślak E, Franczyk-Żarów M et al. (2011). Effects of trans-10,cis-12 and cis-9,trans-11 CLA on atherosclerosis in apoE/LDLRS/S mice. Eur. J. Lipid Sci Technol 113, 572–583.

    Article  CAS  Google Scholar 

  54. Nestel P, Fujii A, Allen T (2006) The cis-9,trans-11 isomer of conjugated linoleic acid (CLA) lowers plasma triglyceride and raises HDL cholesterol concentrations but does not suppress aortic atherosclerosis in diabetic apoE-deficient mice. Atherosclerosis 189: 282–287

    Article  PubMed  CAS  Google Scholar 

  55. Cooper MH, Miller JR, Mitchell PL et al. (2008) Conjugated linoleic acid isomers have no effect on atherosclerosis and adverse effects on lipoprotein and liver lipid metabolism in apoE-/- mice fed a high-cholesterol diet. Atherosclerosis 200: 294–302

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Renata B. Kostogrys.

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Kostogrys, R.B., Franczyk-Żarow, M., Maslak, E. et al. Effects of margarine supplemented with T10C12 and C9T11 CLA on atherosclerosis and steatosis in apoE/LDLR -/- mice. J Nutr Health Aging 16, 482–490 (2012). https://doi.org/10.1007/s12603-011-0354-4

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  • DOI: https://doi.org/10.1007/s12603-011-0354-4

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