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Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces

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Abstract

Enterococcus, a common commensal organism in the human gut, exhibits a dual nature with certain strains offering probiotic benefits, while others are associated with nosocomial infections. In this study, we conducted a comprehensive examination of the genome of Enterococcus mundtii strain 203 to assess its probiotic potential and safety profile. The complete genome sequencing, assembly, and annotation were performed, followed by bioinformatics analysis. Our investigation reveals a detailed characterization of the Enterococcus mundtii 203 genome, originally isolated from camel feces, with a size of 3,053,234 bases and a GC content of 38.4%. Importantly, our analysis suggests that this strain poses no risk as a human pathogen due to the absence of antibiotic resistance determinants and virulence factors. The genome harbors a multitude of genes responsible for lactic acid production, bioactive peptide synthesis, adhesion molecule expression, resistance to harsh gut conditions, and enhancement of host metabolism. These findings underline the potential probiotic functionality of Enterococcus mundtii 203, positioning it as a promising candidate. Notably, our study did not identify any sequences related to insertion elements or CRISPR-Cas fragments.

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The genome sequence assembly project has been deposited at GenBank under accession number JAXGFV000000000.

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Acknowledgements

We would like to thank the UATRS genomics team at the CNRST center for carrying out the sequencing.

Funding

The present work was financially supported by the Hassan II Academy of Science and Technology and CNRST project PPR B 50.

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Authors and Affiliations

  1. Biotechnology and Biomolecular Engineering Research Team, Department of Biology, Faculty of Science and Technology, Abdelmalek Essaâdi University, Tetouan, Morocco

    Soumaya Ahadaf, Safae Azzouz, Ouiam El Galiou, Abdelhay Arakrak & Amin Laglaoui

  2. Biotechnology Research Unit, Regional Center of Agricultural Research of Rabat, National Institute of Agricultural Research, Avenue Ennasr, BP 415 Rabat Principale, 10090, Rabat, Morocco

    Mohamed Akram Errahmouni & Rachid Mentag

Authors
  1. Soumaya Ahadaf
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  2. Safae Azzouz
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  3. Ouiam El Galiou
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  4. Mohamed Akram Errahmouni
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  5. Rachid Mentag
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  6. Abdelhay Arakrak
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  7. Amin Laglaoui
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Soumaya Ahadaf, Rachid Mentag, Mohamed Akram Errahmouni, and Amin Laglaoui. The first draft of the manuscript was written by Soumaya Ahadaf and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Amin Laglaoui.

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Ahadaf, S., Azzouz, S., Galiou, O.E. et al. Genomic Insights Into Enterococcus mundtii 203: A Promising Probiotic Candidate Isolated From Camel Feces. Probiotics & Antimicro. Prot. (2024). https://doi.org/10.1007/s12602-024-10284-6

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