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Influence of Dietary Probiotic and Alpha-Monolaurin on Performance, Egg Quality, Blood Constituents, and Egg Fatty Acids’ Profile in Laying Hens

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Abstract

This work was designed to evaluate the advantages of using multi-strain probiotics feed (Bacillus subtilis, Bacillus licheniformis and Clostridium butyricum) (PRO) and alpha-monolaurin (AML) on laying performance, criteria of egg quality, blood parameters, and yolk fatty acids’ profile in laying hens. One hundred forty of Bovans brown laying hens at 45 weeks old (25th week of egg production) were randomly allocated into four groups, with seven replicates of five birds each in a complete randomized design. The first group was fed a basal diet without feed additives (0 g/kg diet), and the second, third, and fourth groups received diets containing 1 g PRO, 1 g AML, and 1 g PRO + 1 g AML/kg diet, respectively. No significant impacts of PRO, AML, or their mixture on body weight (BW), body weight gain (BWG), feed intake (FI), or egg weight. Egg production, egg mass, and feed conversion ratio (FCR) were enhanced by 1 g PRO/kg and /or 1 g AML/kg supplementation in laying hen diets. Furthermore, egg shape index, eggshell thickness, and yolk color were statistically higher by PRO and AML supplementation at 55 weeks. However, oviduct, infundibulum, and uterus weights were significantly decreased by 1 g PRO or/and 1 g AML. Additionally, total cholesterol, triglycerides, low density lipoprotein (LDL), glucose, and glutamate pyruvate transaminase (GPT) levels were decreased by PRO and AML supplementation. In conclusion, it seems that dietary inclusion with 1 g PRO/kg, 1 g of AML/kg, and 1 g PRO + 1 g AML improved egg production, egg mass, FCR, and yolk fatty acids profile and lowered total cholesterol and malondialdehyde (MDA) contents in laying hens.

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Data Availability

The data presented in this study are available on request from the corresponding authors.

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Acknowledgements

This work was supported by Researchers Supporting Project (RSPD2024R731), King Saud University (Riyadh, Saudi Arabia).

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Authors and Affiliations

  1. Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Kafr El- Sheikh, 333516, Egypt

    Ahmed A. Saleh, Mahmoud Metwally & Mohammed Ragab

  2. School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy

    Livio Galosi

  3. Animals Production Institute, Agriculture Research Center, Ministry of Agriculture, Cairo, 11470, Egypt

    Mahmoud Hamza

  4. Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr El-Sheikh, 33516, Egypt

    Mostafa Shukry

  5. Famex Company, 4-1B Polygon Business Park, Beverly Hills, Sheikh Zayed City, Giza Governorate, Egypt

    Medhat Khairy

  6. Department of Animal Production, Faculty of Agriculture and Food Sciences, Ibb University, Ibb, 70270, Yemen

    Mohammed H. Alzawqari

  7. Department of Animal Production, College of Food & Agriculture Sciences, King Saud University, Riyadh, 11451, Saudi Arabia

    Mahmoud M. Azzam

  8. Poultry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt

    Mahmoud Alagawany

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All authors contributed equally to all works conducted in the present study. All authors have drafted, reviewed, revised, and approved the final manuscript.

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Correspondence to Ahmed A. Saleh or Mahmoud Alagawany.

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The experiment was accepted by the Ethics Committee of the Local Experimental Animals Care Committee and performed under the guidelines of the Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh University, Egypt (Number 4/2016 EC).

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Saleh, A.A., Galosi, L., Metwally, M. et al. Influence of Dietary Probiotic and Alpha-Monolaurin on Performance, Egg Quality, Blood Constituents, and Egg Fatty Acids’ Profile in Laying Hens. Probiotics & Antimicro. Prot. (2024). https://doi.org/10.1007/s12602-024-10260-0

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