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Strain-Specific Anti-Inflammatory Effects of Faecalibacterium prausnitzii Strain KBL1027 in Koreans

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Abstract

Faecalibacterium prausnitzii is one of the most dominant commensal bacteria in the human gut, and certain anti-inflammatory functions have been attributed to a single microbial anti-inflammatory molecule (MAM). Simultaneously, substantial diversity among F. prausnitzii strains is acknowledged, emphasizing the need for strain-level functional studies aimed at developing innovative probiotics. Here, two distinct F. prausnitzii strains, KBL1026 and KBL1027, were isolated from Korean donors, exhibiting notable differences in the relative abundance of F. prausnitzii. Both strains were identified as the core Faecalibacterium amplicon sequence variant (ASV) within the healthy Korean cohort, and their MAM sequences showed a high similarity of 98.6%. However, when a single strain was introduced to mice with dextran sulfate sodium (DSS)-induced colitis, KBL1027 showed the most significant ameliorative effects, including alleviation of colonic inflammation and restoration of gut microbial dysbiosis. Moreover, the supernatant from KBL1027 elevated the secretion of IL-10 cytokine more than that of KBL1026 in mouse bone marrow–derived macrophage (BMDM) cells, suggesting that the strain-specific, anti-inflammatory efficacy of KBL1027 might involve effector compounds other than MAM. Through analysis of the Faecalibacterium pan-genome and comparative genomics, strain-specific functions related to extracellular polysaccharide biosynthesis were identified in KBL1027, which could contribute to the observed morphological disparities. Collectively, our findings highlight the strain-specific, anti-inflammatory functions of F. prausnitzii, even within the same core ASV, emphasizing the influence of their human origin.

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Data Availability

The datasets generated in this study are available in Figshare at https://doi.org/https://doi.org/10.6084/m9.figshare.24105207. All other data that support the findings of this study are available upon reasonable request to the corresponding author.

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Funding

This work was supported by the National Research Foundation of Korea (NRF) (NRF-2022M3A9F3017371, RS-2023-00223831) and the Main Research Program (E0170600-07) of the Korea Food Research Institute (KFRI), funded by the Korean Ministry of Science and Information & Communication Technology (ICT).

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Authors and Affiliations

  1. Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea

    Boram Seo, Kyungchan Jeon, Woon-Ki Kim, You Jin Jang & GwangPyo Ko

  2. Personalized Diet Research Group, Food Functionality Research Division, Korea Food Research Institute, Jeollabuk-do, Republic of Korea

    Boram Seo

  3. Natural Product Informatics Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Republic of Korea

    Kwang Hyun Cha

  4. N-Bio, Seoul National University, Seoul, Republic of Korea

    GwangPyo Ko

  5. Center for Human and Environmental Microbiome, Institute of Health and Environment, Seoul National University, Seoul, Republic of Korea

    GwangPyo Ko

  6. KoBioLabs Inc., Seoul, Republic of Korea

    GwangPyo Ko

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  1. Boram Seo
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Contributions

B.S. designed the study, performed the experiments, and analyzed the data. K.J., W.K., and Y.J.J. assisted in the in vivo mouse experiments. K.H.C. generated the microbiome data from human donor feces for bacterial isolation. B.S. wrote the initial draft of the manuscript. G.K. supervised the study and revised the manuscript accordingly.

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Correspondence to GwangPyo Ko.

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G.K. is the founder of KoBioLabs, Inc. The remaining authors declare that they have no conflict of interest.

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Seo, B., Jeon, K., Kim, WK. et al. Strain-Specific Anti-Inflammatory Effects of Faecalibacterium prausnitzii Strain KBL1027 in Koreans. Probiotics & Antimicro. Prot. (2024). https://doi.org/10.1007/s12602-024-10213-7

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