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Lactiplantibacillus plantarum Strain FLPL05 Promotes Longevity in Mice by Improving Intestinal Barrier

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Abstract

This study aimed to evaluate the effect of oral administration of probiotic Lactiplantibacillus plantarum FLPL05 on the lifespan and intestinal barrier of aged mice. L. plantarum FLPL05 significantly prolonged the lifespan of naturally aged mice, maintained the integrity of intestinal mucosal barrier, and reduced the inflammation level. The analysis of intestinal microbiota revealed that L. plantarum FLPL05 increased the relative abundance of Firmicutes and decreased the abundance of Bacteroides, accompanied by the increased proportions of Lactobacillus and Desulfovibrio in intestinal microbiota as well as the reduced proportions of Roseburia and Parabacteroides. The intestinal proteomics revealed that the oral administration of L. plantarum FLPL05 significantly upregulated the tight junction and simultaneously inhibited the expression of apoptotic-related proteins. The immunohistochemistry results also indicated that L. plantarum FLPL05 promoted the expression of tight junction proteins (ZO-1 and occludin) and reduced the apoptosis of intestinal cells. In addition, L. plantarum FLPL05 and the fermented supernatant increased the activity of HT-29. L. plantarum FLPL05 prolonged the lifespan by improving the health of the intestinal tract after aging and may be a potential probiotic and nutritional supplement for the elderly people.

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Data are available on reasonable request to the authors.

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Funding

This study was funded by the National Natural Science Foundation of China (NSF31260363, 31000048, 31260263).

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Authors and Affiliations

  1. Jiangxi-Oai Joint Research Institute, Nanchang University, 235 Nanjing Donglu, Nanchang, 330047, People’s Republic of China

    Xiaomin Yu, Min Wei, Dong Yang, Hua Wei & Feng Xu

  2. School of Basic Medical Sciences, Nanchang University, Nanchang, 330047, People’s Republic of China

    Xiaomin Yu

  3. College of Basic Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, 330004, People’s Republic of China

    Xiaoli Wu

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  1. Xiaomin Yu
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  2. Min Wei
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  3. Dong Yang
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  4. Xiaoli Wu
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  5. Hua Wei
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  6. Feng Xu
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Contributions

XY and MW performed and designed experiments, XW analyzed data, XY and FX wrote the manuscript performed experiments, and HW and XY edited the manuscript and provided advice. All authors read and approved the final manuscript.

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Correspondence to Hua Wei or Feng Xu.

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Yu, X., Wei, M., Yang, D. et al. Lactiplantibacillus plantarum Strain FLPL05 Promotes Longevity in Mice by Improving Intestinal Barrier. Probiotics & Antimicro. Prot. 15, 1193–1205 (2023). https://doi.org/10.1007/s12602-022-09933-5

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