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Assessment of Two Potential Probiotic Strains As Anti-Obesity Supplements Under High-Fat Feeding Conditions

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Abstract

Obesity is one of the chronic diseases that increase annually and cause cardiovascular disease, which is the main cause of death worldwide. So, this study aims to evaluate the role of the two potential probiotics: Lactiplantibacillus plantarum Pro1 and Lacticaseibacillus rhamnosus Pro2, isolated from the fermented milk and corn silage as anti-obesity supplements. Seventy-five male BALB/c mice were distributed to five groups (control, obesity, obesity plus L. plantarum (OLP), obesity plus L. rhamnosus (OLR) and obesity plus mixture of two strains (OM)). The body weight, lipid profile, histopathology and enzymes of liver were assessed. RT-PCR was used to determine the expression of CYP7A1, ALTG4, TNFα and ROR genes.

The findings show that the obesity group recorded the significant highest value of the body weight, TC, TG, LDL, AST and ALT, while OLP group recorded the significant lowest value. Liver tissue of obesity group has necrosis and fatty changes, while the OLP group was related to the control group. The findings of RT-PCR show non-significant differences between the control group and the OLP group, with significant differences between the control group and the set groups in expression of CYP7A1, ALTG4, TNFα and ROR genes. L. plantarum Pro1 reduced the expression of inflammation genes (TNFα and ROR), and increase the expression of the lipid metabolism genes (CYP7A1, ALTG4) to reduce the inflammatory effects of obesity in the liver, and decrease the cholesterol level in serum. Therefore, L. plantarum Pro1 is useful as anti-obesity supplements and an eliminator of the relevant diseases.

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Authors and Affiliations

  1. Department of Emergency Medical Services, College of Health Sciences in AlQunfudah, Umm Al-Qura University, Mekkah, Saudi Arabia

    Naif ALSuhaymi

  2. Cell Biology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza, Egypt

    Ahmed Mohamed Darwish

  3. Genetics and Cytology Department, Biotechnology Research Institute, National Research Centre, Dokki, Giza, Egypt

    Abd El-Nasser Khattab

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  1. Naif ALSuhaymi
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  2. Ahmed Mohamed Darwish
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Contributions

This study was done in collaboration with all authors. A.K. and A.D. designed this study. N.E., A.D. and A.K. participated in the conduct of the study. N.E. and A.D. analyzed the data. A.D. and A.K. drafted the manuscript. A.D. and N.E. critically revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ahmed Mohamed Darwish.

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The experimental procedure used in this investigation was approved (NRC: 2,020,107) by the Animal Care and Use Committee of National Research Centre in Egypt.

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ALSuhaymi, N., Darwish, A.M. & Khattab, A.EN. Assessment of Two Potential Probiotic Strains As Anti-Obesity Supplements Under High-Fat Feeding Conditions. Probiotics & Antimicro. Prot. 15, 856–867 (2023). https://doi.org/10.1007/s12602-022-09912-w

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