Abstract
Mucositis is one of the most strenuous side effects caused by chemotherapy drugs, such as 5-fluorouracil (5-FU), during the treatment of several types of cancers. The disease is so prevalent and aggressive that many patients cannot resist such symptoms. However, despite its frequency and clinical significance, there is no effective treatment to prevent or treat mucositis. Thus, the use of probiotics as an adjuvant for the treatment has gained prominence. In the present study, we evaluated the effectiveness of oral administration of the Antarctic strain of Rhodotorula mucilaginosa UFMGCB 18,377 as an alternative to minimize side effects of 5-FU-induced mucositis in mice. Body weight, food consumption, stool consistency, and presence of blood in the feces were assessed daily in mice orally treated or not with the yeast and submitted or not to experimental mucositis. Blood, bones, and intestinal tissues and fluid were used to determine intestinal permeability and immunological, microbiological, and histopathological parameters. Treatment with R. mucilaginosa UFMGCB 18,377 was able to decrease clinical signs of the disease, such as reduction of food intake and body weight loss, and also decreased the number of intestinal enterobacteria and intestinal length shortening. Additionally, treatment was able to decrease the levels of MPO and EPO activities and inflammatory infiltrates, as well as the histopathological lesions characteristic of mucositis in the jejunum and ileum. Results of the present study showed that the oral administration of R. mucilaginosa UFMGCB 18,377 protected mice against mucositis induced by 5-FU.
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Data Availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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This work was supported by grants from the Minas Gerais State Research Support Foundation (FAPEMIG, APQ-00593–14) and the Coordination for the Improvement of Higher Education Personnel (CAPES 88887.136384/2017–00 and 88887.314457/2019–00). Financial support was also received from CNPq PROANTAR 442258/2018–6, INCT Criosfera II, and FNDCT. JOPAC was the recipient of a doctorate’s fellowship from CAPES.
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All animal procedures were carried out according to the standards of the Brazilian Society of Laboratory Animal Science/Brazilian College for Animal Experimentation (available at http://www.mctic.gov.br/concea). This work was approved by the Ethics Committee in Animal Experimentation of the Federal University of Minas Gerais (CEUA/UFMG, protocol # 186/2012).
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Coutinho, J.O.P.A., Quintanilha, M.F., Campos, M.R.A. et al. Antarctic Strain of Rhodotorula mucilaginosa UFMGCB 18,377 Attenuates Mucositis Induced by 5-Fluorouracil in Mice. Probiotics & Antimicro. Prot. 14, 486–500 (2022). https://doi.org/10.1007/s12602-021-09817-0
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DOI: https://doi.org/10.1007/s12602-021-09817-0