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In vitro Inhibition of Clinical Isolates of Otitis Media Pathogens by the Probiotic Streptococcus salivarius BLIS K12

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Abstract

Otitis media is a common childhood infection, frequently requiring antibiotics. With high rates of antibiotic prescribing and increasing antibiotic resistance, new strategies in otitis media prevention and treatment are needed. The aim of this study was to assess the in vitro inhibitory activity Streptococcus salivarius BLIS K12 against otitis media pathogens. Efficacy of the bacteriocin activity of S. salivarius BLIS K12 against the otitis media isolates was assessed using the deferred antagonism test. Overall, 48% of pathogenic isolates exhibited some growth inhibition by S. salivarius BLIS K12. S. salivarius BLIS K12 can inhibit the in vitro growth of the most common pathogens.

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Acknowledgements

Blis Technology provided BLIS K12 and the use of lab facilities without cost to the research team.

Funding

The study was investigator led and funded through the Paediatric Department, University of Otago, Christchurch.

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Authors and Affiliations

  1. Department of Otolaryngology, Head and Neck Surgery, Christchurch Public Hospital, Christchurch, New Zealand

    Tzu Yu Chen, Duncan S. Stevenson, Philip A. Bird & Tony Walls

  2. Blis Technologies Ltd, Dunedin, New Zealand

    John D. F. Hale, John R. Tagg, Rohit Jain & Abigail L. Voss

  3. Department of Paediatric Otolaryngology, Starship Children’s Hospital, Auckland, New Zealand

    Nikki Mills

  4. Department of Paediatrics and Child Health, University of Auckland, Auckland, New Zealand

    Emma J. Best

  5. Department of Surgery, University of Otago, Christchurch, New Zealand

    Duncan S. Stevenson & Philip A. Bird

  6. Department of Paediatrics, University of Otago, Christchurch, New Zealand

    Tony Walls

Authors
  1. Tzu Yu Chen
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  2. John D. F. Hale
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  3. John R. Tagg
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  5. Abigail L. Voss
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  6. Nikki Mills
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  7. Emma J. Best
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  8. Duncan S. Stevenson
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  10. Tony Walls
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Corresponding author

Correspondence to Tzu Yu Chen.

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Ethical Approval

The study was approved by the Health and Disability Ethics Committee, New Zealand. Ethics approval number—NTX/11/04/029/AM02.

Informed Consent

Participants in the original OMIVI studies provided written consent for storage of their sample for later research.

Conflict of Interest

Dr. J Hale, Professor J Tagg, Dr. R Jain and A. Voss are paid employees of Blis Technologies. None of the other authors has any conflict of interest to declare.

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Chen, T.Y., Hale, J.D.F., Tagg, J.R. et al. In vitro Inhibition of Clinical Isolates of Otitis Media Pathogens by the Probiotic Streptococcus salivarius BLIS K12. Probiotics & Antimicro. Prot. 13, 734–738 (2021). https://doi.org/10.1007/s12602-020-09719-7

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  • DOI: https://doi.org/10.1007/s12602-020-09719-7

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