A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen’s egg-laying ability.
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This study was supported by the grant from the Russian Science Foundation RSF No.16-16-04032.
This article is original and contains unpublished materials. The corresponding author confirms that all of the other authors have read and approved the manuscript and no ethical issues involved.
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Mazanko, M.S., Makarenko, M.S., Chistyakov, V.A. et al. Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens. Probiotics & Antimicro. Prot. 11, 1324–1329 (2019). https://doi.org/10.1007/s12602-019-9519-y
- Reproductive aging
- Probiotic supplementation