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Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens

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Abstract

A promising approach for slowing down the rate of reproductive aging is the use of probiotic bacteria as a feed additive. In the current study was investigated the influence of the intake of a potential probiotic on the follicle content and expression of vitellogenin genes (vtg1, vtg2, vtg3) in aged hens. RNA was isolated from liver samples collected from 570-day-old laying hens and gene expression levels were measured using RT-PCR. Bacillus subtilis KATMIRA1933 supplementation had a positive effect on the number of formed follicles in hens and also triggered a significant increase in the relative expression levels of vtg1, vtg2, and vtg3. A Bacillus amyloliquefaciens B-1895 enriched diet or a combination of the two strains had a modest effect on both the number of follicles and the expression of vitellogenin genes. Additionally, the study demonstrates that vitellogenin mRNA expression levels can be considered as a biomarker in a convenient approach for analyzing the hen’s egg-laying ability.

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Funding

This study was supported by the grant from the Russian Science Foundation RSF No.16-16-04032.

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Authors and Affiliations

  1. Southern Federal University, Rostov-on-Don, Russia

    Maria S. Mazanko, Maksim S. Makarenko, Vladimir A. Chistyakov, Alexander V. Usatov, Evgeniya V. Prazdnova, Anzhelika B. Bren & Michael L. Chikindas

  2. Povolzhsky Research Institute of Meat and Dairy Industry Production and Processing, Volgograd, Russia

    Ivan F. Gorlov & Zoya B. Komarova

  3. Health Promoting Naturals Laboratory, School of Environmental and Biological Sciences, Rutgers State University, New Brunswick, NJ, USA

    Richard Weeks & Michael L. Chikindas

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  1. Maria S. Mazanko
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  2. Maksim S. Makarenko
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  3. Vladimir A. Chistyakov
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  4. Alexander V. Usatov
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  5. Evgeniya V. Prazdnova
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Correspondence to Maksim S. Makarenko.

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Mazanko, M.S., Makarenko, M.S., Chistyakov, V.A. et al. Probiotic Intake Increases the Expression of Vitellogenin Genes in Laying Hens. Probiotics & Antimicro. Prot. 11, 1324–1329 (2019). https://doi.org/10.1007/s12602-019-9519-y

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