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Evaluation of Probiotic Properties and Prebiotic Utilization Potential of Weissella paramesenteroides Isolated From Fruits

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Abstract

Weissella paramesenteroides has gained a considerable attention as bacteriocin and exopolysaccharide producers. However, potential of W. paramesenteroides to utilize different prebiotics is unexplored area of research. Fruits being vectors of various probiotics, five W. paramesenteroides strains, namely, FX1, FX2, FX5, FX9, and FX12, were isolated from different fruits. They were screened and selected based on their ability to survive at pH 2.5 and in 1.0% sodium taurocholate, high cell surface hydrophobicity, mucin adhesion, bile-induced biofilm formation, antimicrobial activity (AMA) against selected enteropathogens, and prebiotic utilization ability, implicating the functional properties of these strains. In vitro safety evaluation showed that strains were susceptible to antibiotics except vancomycin and did not harbor any virulent traits such as biogenic amine production, hemolysis, and DNase production. Based on their functionality, two strains FX5 and FX9 were selected for prebiotic utilization studies by thin layer chromatography (TLC) and short-chain fatty acids (SCFAs) production by high performance liquid chromatography. TLC profile evinced the ability of these two strains to utilize low molecular weight galactooligosaccharides (GOS) and fructooligosaccharides (FOS), as only the upper low molecular weight fractions were disappeared from cell-free-supernatants (CFS). Enhanced β-galactosidase activity correlated with galactose accumulation in residual CFS of GOS displayed GOS utilization ability. Both the strains exhibited AMA against E. coli and Staph. aureus and high SCFAs production in the presence of prebiotic, suggesting their synbiotic potential. Thus, W. paramesenteroides strains FX5 and FX9 exhibit potential probiotic properties with prebiotic utilization and can be taken forward to evaluate synergistic synbiotic potential in detail.

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Funding

This work was funded by the University Grant Commission (UGC), Government of India, under Major Research Project Scheme (MRP-Major-MICR-2013-35872). Ms. Kinjal Pabari is thankful to UGC for providing research fellowship. Authors would like to thank Center of Excellence (CoE) in Drug Discovery, Saurashtra University, Gujarat, for providing facility for HPLC analysis. Authors are grateful to Friesland Campina, the Netherlands for providing Vivinal GOS and Sweet Town Biotech, Taiwan for providing XOS.

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Authors and Affiliations

  1. Department of Biotechnology, Christ College, Vidya Niketan, Saurashtra University, PO, Rajkot, Gujarat, 360005, India

    Kinjal Pabari, Charmy Kothari & Padma Ambalam

  2. UGC-CAS Department of Biosciences, Saurashtra University, Rajkot, Gujarat, 360005, India

    Kinjal Pabari, Bharatkumar Rajiv Manuel Vyas & Ramesh Kothari

  3. Government Science College, Sector 15, Gandhinagar, India

    Sheetal Pithva

  4. National Institute of Plant Genome Research, Aruna Asaf Marg, Po Box No. 10531, New Delhi, India

    Ravi Kiran Purama

  5. National Agri-Food Biotechnology Institute, S.A.S. Nagar, Punjab, 140306, India

    Kanthi Kiran Kondepudi

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  1. Kinjal Pabari
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Pabari, K., Pithva, S., Kothari, C. et al. Evaluation of Probiotic Properties and Prebiotic Utilization Potential of Weissella paramesenteroides Isolated From Fruits. Probiotics & Antimicro. Prot. 12, 1126–1138 (2020). https://doi.org/10.1007/s12602-019-09630-w

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