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Safety Evaluation and Whole-Genome Annotation of Lactobacillus plantarum Strains from Different Sources with Special Focus on Isolates from Green Tea

Probiotics and Antimicrobial Proteins volume 12, pages 1057–1070 (2020)Cite this article

Abstract

Lactobacillus plantarum shows high intraspecies diversity species, and has one of the largest genome sizes among the lactobacilli. It is adapted to diverse environments and provides a promising potential for various applications. The aim of the study was to investigate the safety and probiotic properties of 18 L. plantarum strains isolated from fermented food products, green tea, and insects. For preliminary safety evaluation the L. plantarum strains were tested for their ability to produce hemolysin and biogenic amines and for their antibiotic resistance. Based on preliminary safety screening, four strains isolated from green tea showed antibiotic resistance below the cut-off MIC values suggested by EFSA, and were selected out of the 18 strains for more detailed studies. Initial selection of strains with putative probiotic potential was determined by their capacity to survive in the human GIT using an in vitro simulation model, and for their adhesion to human Caco-2/TC-7 cell line. Under simulated GIT conditions, all four L. plantarum strains isolated from green tea showed higher survival rates than the control (L. plantarum subsp. plantarum ATCC 14917). All studied strains were genetically identified by 16S rRNA gene sequencing and confirmed to be L. plantarum. In addition, whole-genome sequence analysis of L. plantarum strains APsulloc 331261 and APsulloc 331263 from green tea was performed, and the outcome was compared with the genome of L. plantarum strain WCFS1. The genome was also annotated, and genes related to virulence factors were searched for. The results suggest that L. plantarum strains APsulloc 331261 and APsulloc 331263 can be considered as potential beneficial strains for human and animal applications.

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Acknowledgments

This work was supported by Amore Pacific Corporation, Seoul, South Korea, and HEM, Pohang, South Korea. Support from the Bio&Medical Technology Program of the Korean National Research Foundation (NRF) (No. 2016M3A9A5923160) is gratefully acknowledged. 

Funding

This study received financial support from the National Council on Science and Technology, CONACYT, Mexico, DF, Mexico by providing financial support to KA, involved in the present project.

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Affiliations

  1. Advanced Green Energy and Environment Institute (AGEE), Handong Global University, Handong-ro 558, Pohang-si, Gyungbuk, 37554, South Korea

    Karina Arellano, Jorge Vazquez, Haryung Park, Juhwan Lim, Yosep Ji, Hye-Ji Kang & Wilhelm Heinrich Holzapfel

  2. Holzapfel Effective Microbes Inc, Iui-dong 1286, Suwon-si, Gyeonggi-do, 31286, South Korea

    Yosep Ji & Wilhelm Heinrich Holzapfel

  3. Vital Beautie Research Division, Amorepacific R&D Center, Yonggin-si, Gyeonggi-do, 17074, South Korea

    Donghyun Cho & Hyun Woo Jeong

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  1. Karina Arellano
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Arellano, K., Vazquez, J., Park, H. et al. Safety Evaluation and Whole-Genome Annotation of Lactobacillus plantarum Strains from Different Sources with Special Focus on Isolates from Green Tea. Probiotics & Antimicro. Prot. 12, 1057–1070 (2020). https://doi.org/10.1007/s12602-019-09620-y

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Keywords

  • Lactobacillus plantarum
  • Safety
  • Probiotic
  • Virulence factors