A Multi-strain Potential Probiotic Formulation of GABA-Producing Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 with Antidepressant Effects


Today, a number of studies conclusively show that certain bacterial strains, mainly from the genera Lactobacillus and Bifidobacterium, influence the functioning of the central nervous system, leading to changes in beahvior, nociception and the cognitive abilities of humans and animals. Such strains serve as the basis for developing probiotics with a curative potential for the central nervous system — psychobioitcs. However, the question of how to find such strains and which criteria to use for their selection remains unanswered. Some compounds produced by bacteria, such as gamma-aminobutyric acid (GABA), the main inhibitory neurotransmitter of the central nervous system, are potential mediators between bacterial cells and the host. Previously, we established that some species of Lactobacillus and Bifidobacterium are capable of producing GABA. We presumed that GABA-producing Lactobacillus and Bifidobacterium strains are great candidates to use as psychobiotics. Therefore, we selected the strains Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 as efficient GABA producers. The goal of this work was to assess the probiotic properties of the selected strains as well as their antidepressive effects in mice. We established that the ingestion of the probiotic composition based on the selected strains by BALB/c mice for 2 weeks reduced depressive-like behavior in the forced swimming test; the effect was similar to that of fluoxetine.

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This project was funded by the state budget reserved for “Genetic technologies in biology, medicine, agricultural and environmental activities N 0112-2019-0002.”

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Correspondence to R. A. Yunes.

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The experiments were approved by a Bioethics Committee of the Research Zakusov Institute of Pharmacology. The animals were handled in accordance with the guidelines set by the directive of the Ministry of Health of the Russian Federation (23.08.2010, N2708N).

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The authors declare that they have no conflict of interest.

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Yunes, R.A., Poluektova, E.U., Vasileva, E.V. et al. A Multi-strain Potential Probiotic Formulation of GABA-Producing Lactobacillus plantarum 90sk and Bifidobacterium adolescentis 150 with Antidepressant Effects. Probiotics & Antimicro. Prot. 12, 973–979 (2020). https://doi.org/10.1007/s12602-019-09601-1

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  • Lactobacillus
  • Bifidobacterium
  • Gamma-aminobutiric acid
  • Multi-strains probiotic composition
  • Antidepressant