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Lactobacillus plantarum PS128 Ameliorated Visceral Hypersensitivity in Rats Through the Gut–Brain Axis

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Abstract

Irritable bowel syndrome (IBS) is a common functional gastrointestinal disorder characterized by abdominal pain and alterations in bowel habits. Current treatments for IBS are unsatisfactory due to its multifactorial pathogenesis involving the microbiota–gut–brain axis. Lactobacillus plantarum PS128 (PS128) was reported to exhibit neuromodulatory activity which may be beneficial for improving IBS. This study aimed to investigate the effect of PS128 on visceral hypersensitivity (VH) and the gut–brain axis using a 5-hydroxytryptophan (5-HTP)-induced VH rat model without colonic inflammation induction, mimicking the characteristics of IBS. Male Sprague–Dawley rats were administered with PS128 (109 CFU in 0.2 mL saline/rat/day) or saline (0.2 mL saline/rat/day) for 14 days. Colorectal distension (CRD) with simultaneous electromyography recording was performed 30 min before and 30 min after the 5-HTP injection. Levels of neuropeptides and neurotrophins were analyzed. PS128 significantly reduced VH induced by the 5-HTP injection and CRD. Neurotransmitter protein levels, substance P, CGRP, BDNF, and NGF, were decreased in the dorsal root ganglion but increased in the spinal cord in response to the 5-HTP injection; PS128 reversed these changes. The hypothalamic–pituitary–adrenal axis was modulated by PS128 with decreased corticosterone concentration in serum and the expression of mineralocorticoid receptors in the amygdala. Oral administration of PS128 inhibited 5-HTP-induced VH during CRD. The ameliorative effect on VH suggests the potential application of PS128 for IBS.

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Funding

This research was financially supported by grants from Academic Technology Development Program of the Ministry of Economic Affairs, Republic of China (101-EC-17-A-17-S1-197; 102-EC-17-A-17-S1-197; 103-EC-17-A-17-S1-197), Ministry of Science and Technology, Republic of China (MOST-102-2313-B-010-001-MY3; MOST 107-2320-B-010-003-MY2), National Science Council (NSC 101-2314-B-010-053), and Taipei Veterans General Hospital (V104E13-001-MY2-1).

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  1. Yen-Wenn Liu and Yen-Po Wang contributed equally to this work.

Authors and Affiliations

  1. Institute of Biochemistry and Molecular Biology, National Yang-Ming University, No. 155, Sec. 2, Linong St., Beitou Dist., Taipei, 11221, Taiwan

    Yen-Wenn Liu, Hsu-Fang Yen, Wen-Jian Tzeng & Ying-Chieh Tsai

  2. Microbiome Research Center, National Yang-Ming University, No. 155, Sec. 2, Linong St., Beitou Dist., Taipei, 11221, Taiwan

    Yen-Wenn Liu & Ying-Chieh Tsai

  3. Endoscopy Center for Diagnosis and Treatment, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan

    Yen-Po Wang & Ching-Liang Lu

  4. Institute of Brain Science, National Yang-Ming University, No. 155, Sec. 2, Linong St., Beitou Dist., Taipei, 11221, Taiwan

    Yen-Po Wang, Pei-Yi Liu & Ching-Liang Lu

  5. School of Medicine, National Yang-Ming University, No. 155, Sec. 2, Linong St., Beitou Dist., Taipei, 11221, Taiwan

    Yen-Po Wang, Chia-Fen Tsai, Han-Chieh Lin, Fa-Yauh Lee & Ching-Liang Lu

  6. Department of Psychiatry, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan

    Chia-Fen Tsai

  7. Division of Gastroenterology, Taipei Veterans General Hospital, No. 201, Sec. 2, Shipai Rd., Beitou Dist., Taipei, 11217, Taiwan

    Han-Chieh Lin, Fa-Yauh Lee & Ching-Liang Lu

  8. Bened Biomedical Co., Ltd., Taipei, Taiwan

    One-Jang Jeng

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  1. Yen-Wenn Liu
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Liu, YW., Wang, YP., Yen, HF. et al. Lactobacillus plantarum PS128 Ameliorated Visceral Hypersensitivity in Rats Through the Gut–Brain Axis. Probiotics & Antimicro. Prot. 12, 980–993 (2020). https://doi.org/10.1007/s12602-019-09595-w

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