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Partial Evaluation of Autochthonous Probiotic Potential of the Gut Microbiota of Seriola lalandi

Probiotics and Antimicrobial Proteins volume 12, pages 672–682 (2020)Cite this article

Abstract

Seriola lalandi is an economically important species that is globally distributed in temperate and subtropical marine waters. Aquaculture production of this species has had problems associated with intensive fish farming, such as disease outbreaks or nutritional deficiencies causing high mortality. Intestinal microbiota are involved in many processes that benefit a host, such as disease control, stimulation of the immune response, and the promotion of nutrient metabolism. The aim of this study is to evaluate the in vitro probiotic properties of bacteria isolated from the intestinal content of wild Seriola lalandi. The probiotic potential was evaluated in terms of (i) the antimicrobial activity against vibrios causing outbreaks in farmed fish; (ii) the ability to stimulate genes related to an innate immune response in fish; and (iii) antibiotic resistance. Nineteen isolates identified as Pseudomonas, Shewanella, Psychrobacter, and Acinetobacter showed antimicrobial activity and significant relative expression of cytokines, serum amyloid A protein (SAA), hepcidin, and lysozyme. A positive correlation was observed between the levels of expression and the bacterial load after 24 h of exposure. Pseudomonas isolates showed a level of antibiotic resistance. In conclusion, isolates of the genera Shewanella, Psychrobacter, and Acinetobacter could serve as potential probiotics in S. lalandi culture.

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Acknowledgments

CR would like to acknowledge the National Commission of Scientific and Technologic Research (CONICYT) for the funding through the National PhD funding program.

Funding

This project was funded by a research grant from FONDECYT 1140734 and 1171129 (Fondo Nacional de Desarrollo Científico y Tecnológico, Chile) and supported by project Aquapacifico 15PCTI-46284 from Corfo.

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Affiliations

  1. Laboratorio de Biotecnología de los Alimentos, Instituto de Nutrición y Tecnología de los Alimentos, Universidad de Chile, Santiago, Chile

    Carolina Ramírez & Jaime Romero

  2. Doctorado en Acuicultura, Programa Cooperativo Universidad de Chile, Universidad Católica del Norte, Pontificia Universidad Católica de Valparaíso, Santiago, Chile

    Carolina Ramírez

  3. Laboratorio de Patobiología Acuática, Departamento de Acuicultura, Universidad Católica del Norte, Coquimbo, Chile

    Rodrigo Rojas

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  1. Carolina Ramírez
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Contributions

CR performed microbial isolation, DNA and RNA extraction, and data analysis and interpretation and wrote the original draft. RR performed the antimicrobial activity and antibiotic resistance studies and reviewed and edited. JR designed the work and reviewed and edited manuscript. All authors have made intellectual contributions to the work and approved it for publication.

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Correspondence to Jaime Romero.

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Ramírez, C., Rojas, R. & Romero, J. Partial Evaluation of Autochthonous Probiotic Potential of the Gut Microbiota of Seriola lalandi. Probiotics & Antimicro. Prot. 12, 672–682 (2020). https://doi.org/10.1007/s12602-019-09550-9

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Keywords

  • Probiotic
  • Microbiota
  • Antimicrobial activity
  • Immune-related genes
  • Seriola lalandi