Probiotics and Antimicrobial Proteins

, Volume 11, Issue 3, pp 966–972 | Cite as

The Use of Two Bifidobacterium Strains Enhanced Growth Performance and Nutrient Utilization of Rainbow Trout (Oncorhynchus mykiss) Fry

  • Javad SahandiEmail author
  • Hojatollah Jafaryan
  • Mehdi Soltani
  • Pouneh Ebrahimi


This study aims to evaluate the effects of Bifidobacterium animalis PTCC-1631 and Bifidobacterium lactis PTCC-1736 on growth, nutrient digestibility, carcass composition, and intestinal microbiota of Oncorhynchus mykiss fry, which had initial body weights of 0.58 ± 0.19 g. Four iso-nitrogenous diets with probiotics, namely T1 (1 × 107 CFU g−1), T2 (2 × 107 CFU g−1), T3 (3 × 107 CFU g−1), and control without probiotics were obtained. Total 480 fish were randomly divided in four treatments (three experimental treatments and a control), each with 4 replicates including 16 tanks each filled with 15 L−1 of water (water exchange rate of 2 L min−1 and aerated with air stone) with a density of 2 fry L−1. The water mean temperature was 17.66 ± 1.33 °C, and the mean pH value was 7.63 ± 0.08. After eight weeks of the experiment T1 that fed with a diet contained the lowest bacterial concentration showed higher growth, nutrient utilization, digestibility, and lower feed conversion ratio (P < 0.05) in comparison with T2, T3, and the control. Also, the highest count of lactobacillus bacteria was observed in T1 that was fed with a diet containing the lowest probiotic. The lowest concentration of Bifidobacterium strains in this study induced better growth and increased digestion and nutrient utilization in trout fry.


Bifidobacterium Oncorhynchus mykiss Fry Nutrient Digestibility 



The authors are thankful of Prof. Brian Austin for useful comments, and also, thank all employees of GKU especially Dr. Bayat-Kohsar director of laboratories and experts Mr. Jafarzadeh, Mr. Hosseini, and Mrs. Saravani for providing all the necessary facilities. This work was carried out as the master course thesis at Department of Fisheries and Forestry, Faculty of Natural Resources, Gonbad Kavous University, Gonbad, Iran.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

All applicable international, national, and guidelines for the care and use of animals were followed.

Informed Consent

Informed consent was obtained from all individual participants included in the study.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Fisheries and Forestry, Faculty of Natural ResourcesGonbad Kavous UniversityGonbad-e KavousIran
  2. 2.Department of Marine Environmental and Ecological Science, College of Marine Life ScienceOcean University of ChinaQingdaoChina
  3. 3.Department of Aquatic Animal Health and Disease, Faculty of VeterinaryUniversity of TehranTehranIran
  4. 4.Department of Chemistry, Faculty of Basic SciencesGolestan UniversityGorganIran

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