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Consumption of Probiotic Lactobacillus fermentum MTCC: 5898-Fermented Milk Attenuates Dyslipidemia, Oxidative Stress, and Inflammation in Male Rats Fed on Cholesterol-Enriched Diet

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Abstract

There is a growing and alarming prevalence that increased serum cholesterol is closely related to increased cardiovascular disease risk. Probiotic consumption could be a safe and natural strategy to combat. Therefore, we sought to examine the cholesterol-lowering potential of co-supplementation of probiotic bacteria Lactobacillus fermentum MTCC: 5898-fermented buffalo milk (2.5% fat) in rats fed cholesterol-enriched diet. Male Wistar rats were divided into three groups on the basis of feed, viz. group 1, fed standard diet (SD); group 2, fed cholesterol-enriched diet (CED); and group 3, fed cholesterol-enriched diet along with L. fermentum MTCC: 5898-fermented milk (CED+LF) for 90 days. At the endpoint, significantly higher levels of serum total cholesterol, low-density lipoprotein cholesterol, triacylglycerols, very low density lipoprotein cholesterol, atherogenic index, coronary artery risk index, hepatic lipids, lipid peroxidation, and mRNA expression of inflammatory cytokines (TNF-α and IL-6) in the liver while significantly lower levels of serum high-density lipoprotein cholesterol and anti-oxidative enzyme activities, catalase, superoxide dismutase, and glutathione peroxidase in the liver and kidney were observed in the CED group compared to the SD group. Compared to the CED group, these adverse physiological alterations were found significantly improved in the CED+LF group. Hence, this study proposes that L. fermentum MTCC: 5898 is a potential probiotic bacteria that can be consumed to tackle hypercholesterolemia.

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Acknowledgements

The authors are grateful to the Director of ICAR-National Dairy Research Institute, Karnal, for providing laboratory facilities to carry out this work.

Funding

Funding is provided by ICAR-National Dairy Research Institute, Karnal.

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Authors and Affiliations

  1. Lab-146, Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India

    Radha Yadav, Suhail Hakeem Khan, Sunita Meena, Rajeev Kapila & Suman Kapila

  2. Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria

    Sanusi Bello Mada

Authors
  1. Radha Yadav
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  2. Suhail Hakeem Khan
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  3. Sanusi Bello Mada
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  4. Sunita Meena
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  5. Rajeev Kapila
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  6. Suman Kapila
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Corresponding author

Correspondence to Suman Kapila.

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Ethical Approval

The study was approved by the Institute’s Animal Ethical Committee (IAEC) for Animal Experiments of National Dairy Research Institute (IAEC No. 101/16 dated 21 April 2016), Karnal, Haryana, India.

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The authors declare that they have no conflict of interest.

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Yadav, R., Khan, S.H., Mada, S.B. et al. Consumption of Probiotic Lactobacillus fermentum MTCC: 5898-Fermented Milk Attenuates Dyslipidemia, Oxidative Stress, and Inflammation in Male Rats Fed on Cholesterol-Enriched Diet. Probiotics & Antimicro. Prot. 11, 509–518 (2019). https://doi.org/10.1007/s12602-018-9429-4

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  • DOI: https://doi.org/10.1007/s12602-018-9429-4

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