Skip to main content
SpringerLink
Log in

Effect of Dietary Supplementation of Zinc and Multi-Microbe Probiotic on Growth Traits and Alteration of Intestinal Architecture in Broiler

Probiotics and Antimicrobial Proteins Aims and scope Submit manuscript

Cite this article

Abstract

This experiment aimed to investigate the effect of different levels of zinc (Zn) and a probiotic on the growth and intestinal health of broiler chickens. One hundred ninety-two-day-old broiler chicks were randomly assigned to 6 groups. The birds were supplemented with Zn (30 and 60 mg/kg) and a probiotic mixture alone or in combination with Zn. The results revealed that the broilers receiving a probiotic mixture alone or in combination with Zn (30 and 60 mg) increased (P < 0.05) final body weight, feed conversion ratio (FCR), and total goblet cell count in duodenum compared with the control group. Morphometry of the duodenum also revealed an increase (P < 0.05) in villus height (VH) and the ratio of VH and crypt depth in all the supplemented groups, whereas ileal VH increased (P < 0.05) only in Zn60 group when compared with control. Based on our results, we concluded that compared to the individual supplementation, the combined effect of Zn and probiotic mixture was superior in improving the growth performance and histomorphology of the intestine; however, the effect of two levels of Zn did not vary when combined with probiotic.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

We’re sorry, something doesn't seem to be working properly.

Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. Khan RU, Naz S, Javdani M, Nikousefat Z, Selvaggi M, Tufarelli V, Laudadio V (2012) The use of turmeric (Curcuma longa) in poultry feed. World's Poultry Sci J 68(1):97–103

    Article  Google Scholar 

  2. Alzawqari M, Al-Baddany A, Al-Baadani H, Alhidary I, Khan RU, Aqil G, Abdurab A (2016) Effect of feeding dried sweet orange (Citrus sinensis) peel and lemon grass (Cymbopogon citratus) leaves on growth performance, carcass traits, serum metabolites and antioxidant status in broiler during the finisher phase. Environ Sci Poll Res 23(17):17077–17082

    Article  CAS  Google Scholar 

  3. Chand N, Faheem H, Khan RU, Qureshi MS, Alhidary IA, Abudabos AM (2016) Anticoccidial effect of mananoligosacharide against experimentally induced coccidiosis in broiler. Environ Sci Poll Res 23(14):14414–14421

    Article  CAS  Google Scholar 

  4. Rahman S, Khan S, Chand N, Sadique U, Khan RU (2017) In vivo effects of Allium cepa L. on the selected gut microflora and intestinal histomorphology in broiler. Acta Histochem 119:446–450

    Article  CAS  Google Scholar 

  5. Khan RU, Naz S (2013) The applications of probiotics in poultry production. World's Poultry Sci J 69(3):621–632

    Article  Google Scholar 

  6. Khan RU, Naz S, Dhama K, Karthik K, Tiwari R, Abdelrahman MM, Alhidary IA, Zahoor A (2016) Direct-fed microbial: beneficial applications, modes of action and prospects as a safe tool for enhancing ruminant production and safeguarding health. Inter J Pharmacol 12(3):220–231

    Article  CAS  Google Scholar 

  7. Khan RU, Rahman Z, Javed I, Muhammad F (2014) Effect of vitamins, protein level and probiotics on immune response of moulted male broiler breeders. J Anim Physiol Anim Nutr 98(4):620–627

    Article  CAS  Google Scholar 

  8. Abudabos AM, Alyemni AH, Dafalla YM, Khan RU (2017) Effect of organic acid blend and Bacillus subtilis alone or in combination on growth traits, blood biochemical and antioxidant status in broilers exposed to Salmonella typhimurium challenge during the starter phase. J Appl Anim Res 45(1):538–542

    Article  CAS  Google Scholar 

  9. Perić L, Milošević N, Žikić D, Bjedov S, Cvetković D, Markov S, Mohnl M, Steiner T (2010) Effects of probiotic and phytogenic products on performance, gut morphology and cecal microflora of broiler chickens. Archiv Tierzucht 53:350–359

    Google Scholar 

  10. Sen S, Ingale S, Kim Y, Kim J, Kim K, Lohakare J, Kim E, Kim H, Ryu M, Kwon I (2012) Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Res Vet Sci 93(1):264–268

    Article  PubMed  Google Scholar 

  11. Rahman H, Qureshi M, Khan R (2014) Influence of dietary zinc on semen traits and seminal plasma antioxidant enzymes and trace minerals of Beetal bucks. Rep Dom Anim 49(6):1004–1007

    Article  CAS  Google Scholar 

  12. Naz S, Idris M, Khalique M, Alhidary I, Abdelrahman M, Khan R, Chand N, Farooq U, Ahmad S (2016) The activity and use of zinc in poultry diets. World's Poultry Sci J 72(1):159–167

    Article  Google Scholar 

  13. Chand N, Naz S, Khan A, Khan S, Khan RU (2014) Performance traits and immune response of broiler chicks treated with zinc and ascorbic acid supplementation during cyclic heat stress. Int J Biometeorol 58(10):2153–2157

    Article  PubMed  Google Scholar 

  14. Khan RU, Nikousefat Z, Javdani M, Tufarelli V, Laudadio V (2011) Zinc-induced moulting: production and physiology. World's Poultry Sci J 67(3):497–506

    Article  Google Scholar 

  15. Tako E, Ferket PR, Uni Z (2005) Changes in chicken intestinal zinc exporter mRNA expression and small intestinal functionality following intra-amniotic zinc-methionine administration. J Nutr Biochem 16(6):339–346

    Article  CAS  PubMed  Google Scholar 

  16. Khan RU, Rahman Z, Javed I, Muhammad F (2013) Supplementation of dietary vitamins, protein and probiotics on semen traits and immunohistochemical study of pituitary hormones in zinc-induced molted broiler breeders. Acta Histoch 115(7):698–704

    Article  CAS  Google Scholar 

  17. Duff M, Ettarh RR (2002) Crypt cell production rate in the small intestine of the zinc-supplemented mouse. Cell Tiss Org 172(1):21–28

    Article  CAS  Google Scholar 

  18. Lambert JC, Zhou Z, Wang L, Song Z, McClain CJ, Kang YJ (2004) Preservation of intestinal structural integrity by zinc is independent of metallothionein in alcohol-intoxicated mice. Amer J Pathol 164(6):1959–1966

    Article  CAS  Google Scholar 

  19. Council NR (1994) Nutrient requirements of poultry.,(National Academy Press: Washington, DC). USA,

  20. Alhidary IA, Zahoor A (2016) Direct-fed microbial: beneficial applications modes of action and prospects as a safe tool for enhancing ruminant production and safeguarding health. Intern J Pharmacol 12:220–231

    Article  CAS  Google Scholar 

  21. Sahin K, Smith M, Onderci M, Sahin N, Gursu M, Kucuk O (2005) Supplementation of zinc from organic or inorganic source improves performance and antioxidant status of heat-distressed quail. Poultry Sci 84(6):882–887

    Article  CAS  Google Scholar 

  22. Önol AG, Sari M, Oğuz FK, Gülcan B, Erbaş G (2004) The effects of dietary probiotic supplementation on some productivity and blood parameters of laying quails raised under constant heat stress. Turk J Vet Anim Sci 27(6):1397–1402

    Google Scholar 

  23. Bombá A, Nemcová Rr, Mudroňová D, Guba P (2002) The possibilities of potentiating the efficacy of probiotics. Trends Food Sci Technol 13(4):121–126

  24. Tesán F, Hernández F, Torti H, Massot F, Huarte M, de Celis ER, Barreiro MA, Weill R, Cremaschi G, Boccio J (2011) Glycine-stabilized zinc gluconate has similar bioavailability than zinc sulfate in a zinc fortified probiotic food. Open Nutr J 4:136–143

    Google Scholar 

  25. Kabir SL, Rahman MM, Rahman M, Rahman M, Ahmed S (2004) The dynamics of probiotics on growth performance and immune response in broilers. Int J Poult Sci 3(5):361–364

    Article  Google Scholar 

  26. Deniz G, Orman A, Cetinkaya F, Gencoglu H, Meral Y, Turkmen I (2011) Effects of probiotic (Bacillus subtilis DSM 17299) supplementation on the caecal microflora and performance in broiler chickens. Revue Méd Vét 162(11):538–545

    CAS  Google Scholar 

  27. Sahraei M, Janmohammadi H (2014) Effect of dietary supplementation by synbiotic and different zinc sources on broiler chicken performance, immune system and intestinal morphology. J. Vet Res 69(3):271–282

    Google Scholar 

  28. Korének M, Koréneková B, Vaško L, Skalická M, Naď P, Šutiak V, Neuschl J (2007) Effects of cadmium and zinc on the activity of chymotrypsin and trypsin in droppings of Japanese quails. Intern J Environ Stud 64(2):221–227

    Article  CAS  Google Scholar 

  29. Mudroňová D, Nemcová R, Lauková A, Koščová J, Strompfová V, Györyová K, Szunyogová E, Lazar G (2006) Effect of Lactobacillus fermentum alone, and in combination with zinc (II) propionate on Salmonella enterica serovar Düsseldorf in Japanese quails. Biologia 61(6):797–801

    Article  Google Scholar 

  30. Fuentes C, Orozco L, Vicente J, Velasco X, Menconi A (2013) Effect of a lactic acid bacteria based probiotic, Floramax-B11®, on performance, bone qualities, and morphometric analysis of broiler chickens: an economic analysis. Biol Syst 12:322–327

    Google Scholar 

  31. Yu B, Liu J, Chiou M, Hsu Y, Chiou P (2007) The effects of probiotic Lactobacillus reuteri Pg4 strain on intestinal characteristics and performance in broilers. Asian-Aust J Anim Sci 20(8):1243–1251

    Article  CAS  Google Scholar 

  32. Awad W, Ghareeb K, Böhm J (2010) Effect of addition of a probiotic micro-organism to broiler diet on intestinal mucosal architecture and electrophysiological parameters. J Anim Physiol Anim Nutr 94(4):486–494

    Article  CAS  Google Scholar 

  33. Jin L, Ho Y, Abdullah N, Jalaludin S (2000) Digestive and bacterial enzyme activities in broilers fed diets supplemented with Lactobacillus cultures. Poultry Sci 79(6):886–891

    Article  CAS  Google Scholar 

  34. Awad W, Ghareeb K, Abdel-Raheem S, Böhm J (2009) Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poultry Sci 88(1):49–56

    Article  CAS  Google Scholar 

  35. Zhang B, Shao Y, Liu D, Yin P, Guo Y, Yuan J (2012) Zinc prevents Salmonella enterica serovar typhimurium-induced loss of intestinal mucosal barrier function in broiler chickens. Avian Pathol 41(4):361–367

    Article  CAS  PubMed  Google Scholar 

  36. Hu C, Gu L, Luan Z, Song J, Zhu K (2012) Effects of montmorillonite–zinc oxide hybrid on performance, diarrhea, intestinal permeability and morphology of weanling pigs. Anim Feed Sci Technol 177(1):108–115

    Article  CAS  Google Scholar 

  37. Tanweer AJ, Chand N, Saddique U, Bailey CA, Khan RU (2014) Antiparasitic effect of wild rue (Peganum harmala L,) against experimentally induced coccidiosis in broiler chicks. Parasitol Res 113:2951–2960

    Article  PubMed  Google Scholar 

  38. Aliakbarpour H, Chamani M, Rahimi G, Sadeghi A, Qujeq D (2012) The Bacillus subtilis and lactic acid bacteria probiotics influences intestinal mucin gene expression, histomorphology and growth performance in broilers. Asian-Aust J Anim Sci 25(9):1285–1293

    Article  CAS  Google Scholar 

  39. Rahimi S, Grimes J, Fletcher O, Oviedo E, Sheldon B (2009) Effect of a direct-fed microbial (Primalac) on structure and ultrastructure of small intestine in turkey poults. Poultry Sci 88(3):491–503

    Article  CAS  Google Scholar 

  40. Baurhoo B, Goldflus F, Zhao X (2009) Purified cell wall of Saccharomyces cerevisiae increases protection against intestinal pathogens in broiler chickens. Intern J Poultry Sci 8(2):133–137

    Article  Google Scholar 

  41. MacDonald RS (2000) The role of zinc in growth and cell proliferation. J Nutr 130(5):1500S–1508S

    Article  CAS  PubMed  Google Scholar 

  42. Liu P, Pieper R, Tedin L, Martin L, Meyer W, Rieger J, Plendl J, Vahjen W, Zentek J (2014) Effect of dietary zinc oxide on jejunal morphological and immunological characteristics in weaned piglets. J Anim Sci 92(11):5009–5018

    Article  CAS  PubMed  Google Scholar 

  43. Solis de Los Santos F, Donoghue A, Farnell M, Huff G, Huff W, Donoghue D (2007) Gastrointestinal maturation is accelerated in turkey poults supplemented with a mannan-oligosaccharide yeast extract (Alphamune). Poultry Sci 86(5):921–930

    Article  CAS  Google Scholar 

  44. Sharma R, Schumacher U, Ronaasen V, Coates M (1995) Rat intestinal mucosal responses to a microbial flora and different diets. Gut 36(2):209–214

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We acknowledge the Department of Poultry Production, University of Veterinary and Animal Sciences, Lahore (Pakistan) for provision of broiler experimental house and other facilities for conduction of the experimental trial.

Author information

Authors and Affiliations

  1. Department of Animal Health, Faculty of Animal Husbandry & Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan

    Muqader Shah & Rifat Ullah Khan

  2. Department of Anatomy and Histology, Faculty of Biosciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Muqader Shah, Hafsa Zaneb, Saima Masood, Saima Ashraf, Arbab Sikandar & Hafiz Faseeh Ur Rehman

  3. Department of Physiology, Faculty of Biosciences, University of Veterinary and Animal Sciences, Lahore, Pakistan

    Habib Ur Rehman

Authors
  1. Muqader Shah
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hafsa Zaneb
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Saima Masood
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rifat Ullah Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Saima Ashraf
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Arbab Sikandar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hafiz Faseeh Ur Rehman
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Habib Ur Rehman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hafsa Zaneb.

Ethics declarations

Conflict of Interest

We have no personal or financial linkages with the commercial organization that can affect our results. We also have no any personal interest in any product that can influence our research work.

Rights and permissions

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shah, M., Zaneb, H., Masood, S. et al. Effect of Dietary Supplementation of Zinc and Multi-Microbe Probiotic on Growth Traits and Alteration of Intestinal Architecture in Broiler. Probiotics & Antimicro. Prot. 11, 931–937 (2019). https://doi.org/10.1007/s12602-018-9424-9

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12602-018-9424-9

Keywords

search

Navigation