Isolation and Preliminary Screening of a Weissella confusa Strain from Giant Panda (Ailuropoda melanoleuca)
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Weissella confusa has recently received attention for its probiotic potential. Some W. confusa and Weissella cibaria strains isolated from fermented foods show favorable probiotic effects. However, the probiotic properties of W. confusa isolated from giant panda remain unreported to date. Thus, this study isolated a W. confusa strain from giant panda feces and then investigated its characteristics and probiotic properties. A lactic acid bacteria strain was isolated from giant panda fecal samples. The isolated strain was screened by in vitro probiotic property tests, including in vitro antimicrobial test, antioxidant test, surface hydrophobicity, and stress resistance. On the basis of biochemical identification and 16S rDNA sequencing, the W. confusa strain was identified as BSP201703. This Weissella confusa strain can survive at pH 2 and 0.3% (w/v) concentration of bile salt environment and inhibit common intestinal pathogens. It also possesses an in vitro antioxidant capacity, a high auto-aggregation ability, and a high surface hydrophobicity. BSP201703 might serve as a probiotic to giant pandas.
KeywordsGiant panda Weisslella confusa Probiotic properties Screening
The present study was supported by National Natural Science Foundation of China (No. 31672318) and the Funded Project of Chengdu giant panda Breeding Research Foundation (No. CPF2014-15 and CPF2015-16).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 1.Liu Q, Ni X, Wang Q, Peng Z, Niu L, Wang H, Zhou Y, Sun H, Pan K, Jing B, Zeng D (2017) Lactobacillus plantarum BSGP201683 Isolated from Giant panda feces attenuated inflammation and improved gut microflora in mice challenged with Enterotoxigenic Escherichia coli. Front Microbiol 8:1885. https://doi.org/10.3389/fmicb.2017.01885 CrossRefGoogle Scholar
- 3.Collins MD, Samelis J, Metaxopoulos J, Wallbanks S (1993) Taxonomic studies on some Leuconostoc-like organisms from fermrnted sausages:description of a new genus Weissella for the Leuconostoc paramesenteroides group of species. J Appl Bacteriol 75:595–603. https://doi.org/10.1111/j.1365-2672.1993.tb01600.x CrossRefGoogle Scholar
- 4.Abriouel H, Lerma LL, Casado Munoz Mdel C, Montoro BP, Kabisch J, Pichner R, Cho GS, Neve H, Fusco V, Franz CM, Galvez A, Benomar N (2015) The controversial nature of the Weissella genus: technological and functional aspects versus whole genome analysis-based pathogenic potential for their application in food and health. Front Microbiol 6:1197. https://doi.org/10.3389/fmicb.2015.01197 CrossRefGoogle Scholar
- 12.Silva MS, Ramos CL, González-Avila M, Gschaedler A, Arrizon J, Schwan RF, Dias DR (2017) Probiotic properties of Weissella cibaria and Leuconostoc citreum isolated from tejuino – a typical Mexican beverage. LWT Food Sci Technol 86:227–232. https://doi.org/10.1016/j.lwt.2017.08.009 CrossRefGoogle Scholar
- 22.Xing J, Wang G, Zhang Q, Liu X, Gu Z, Zhang H, Chen YQ, Chen W (2015) Determining antioxidant activities of lactobacilli cell-free supernatants by cellular antioxidant assay: a comparison with traditional methods. PLoS One 10(3):e0119058. https://doi.org/10.1371/journal.pone.0119058 CrossRefGoogle Scholar
- 25.Martin R, Miquel S, Benevides L, Bridonneau C, Robert V, Hudault S, Chain F, Berteau O, Azevedo V, Chatel JM, Sokol H, Bermudez-Humaran LG, Thomas M, Langella P (2017) Functional characterization of novel Faecalibacterium prausnitzii strains isolated from healthy volunteers: a step forward in the use of F. prausnitzii as a next-generation probiotic. Front Microbiol 8:1226. https://doi.org/10.3389/fmicb.2017.01226 CrossRefGoogle Scholar
- 27.Zhou S, Qu Y, Huang J, Huang Y, Li D, Zhang H (2017) A summary of researches on the wild Giant panda population dynamics (in Chinese). J Sichuan For Sci Technol 38(2):19–33. (周世强,屈元元,黄金燕,黄强,李德生 and 张和民 (2017) 野生大熊猫种群动态的研究综述. 四川林业科技 38(2):19-33). https://doi.org/10.16779/j.cnki.1003-5508.2017.02.004 Google Scholar
- 28.Qing X, Zeng D, Wang H, Ni X, Liu L, Lai J, Khalique A, Pan K, Jing B (2017) Preventing subclinical necrotic enteritis through Lactobacillus johnsonii BS15 by ameliorating lipid metabolism and intestinal microflora in broiler chickens. AMB Express 7(1):139. https://doi.org/10.1186/s13568-017-0439-5 CrossRefGoogle Scholar
- 29.Wang H, Ni X, Qing X, Zeng D, Luo M, Liu L, Li G, Pan K, Jing B (2017) Live probiotic Lactobacillus johnsonii BS15 promotes growth performance and lowers fat deposition by improving lipid metabolism, intestinal development, and gut microflora in broilers. Front Microbiol 8:1073. https://doi.org/10.3389/fmicb.2017.01073 CrossRefGoogle Scholar
- 31.Lim SK, Kwon MS, Lee J, Oh YJ, Jang JY, Lee JH, Park HW, Nam YD, Seo MJ, Roh SW, Choi HJ (2017) Weissella cibaria WIKIM28 ameliorates atopic dermatitis-like skin lesions by inducing tolerogenic dendritic cells and regulatory T cells in BALB/c mice. Sci Rep 7:40040. https://doi.org/10.1038/srep40040 CrossRefGoogle Scholar
- 36.Shin J-H, Lee J-S, Seo J-G (2015) Assessment of cell adhesion, cell surface hydrophobicity, autoaggregation, and lipopolysaccharide-binding properties of live and heat-killed Lactobacillus acidophilus CBT LA1. Korean J Microbiol 51(3):241–248. https://doi.org/10.7845/kjm.2015.5029 CrossRefGoogle Scholar
- 37.Grigoryan S, Bazukyan I, Trchounian A (2017) Aggregation and adhesion activity of lactobacilli isolated from fermented products in vitro and in vivo: a potential probiotic strain. Probiotics Antimicrob Proteins. https://doi.org/10.1007/s12602-017-9283-9
- 39.Son SH, Jeon HL, Yang SJ, Lee NK, Paik HD (2017) In vitro characterization of Lactobacillus brevis KU15006, an isolate from kimchi, reveals anti-adhesion activity against foodborne pathogens and antidiabetic properties. Microb Pathog 112:135–141. https://doi.org/10.1016/j.micpath.2017.09.053 CrossRefGoogle Scholar