Abstract
Probiotic bacteria or their antimicrobial proteinaceous substances called bacteriocins (enterocins) hold promising prophylactic potential for animal breeding. This study present the results achieved after application of Enterocin M in horses. Enterocin M has never been applied to horses before. Clinically healthy horses (10) were involved in this pilot experiment. They were placed in the stables of the University of Veterinary Medicine and Pharmacy, Košice, Slovakia, with the approval of the University Ethics Committee. The animals were fed twice a day with hay and oats, or alternatively grazed with access to water ad libitum. The experiment lasted 6 weeks. Sampling was performed at the start of the experiment, at day 0–1, at day 21 (3 weeks of Enterocin M application), and at day 42 (3 weeks of cessation). Feces were sampled directly from the rectum and blood from the vena jugularis; the samples were immediately treated and/or stored for analyses. Each horse itself represented a control animal (compared to its status at the start of the experiment, day 0–1). After initial sampling, the horses were administered 100 μl of Ent M (precipitate, 12,800 AU/ml) in a small feed bolus to ensure it was consumed; Ent M was applied for 3 weeks (21 days). Fecal samples were treated using the standard microbial dilution method; phagocytic activity was assessed with standard and flow cytometry; biochemistry and metabolic profiles were tested using commercial kits and standard methods. Administration of Ent M led to mathematical reduction of coliforms, campylobacters (abP < 0.05), and significant reduction of Clostridium spp. (abP < 0.001, bcP < 0.001); increase of PA values was noted (P < 0.05, P < 0.0001); no negative influence on hydrolytic enzyme profile or biochemical blood parameters was noted.
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Acknowledgements
We are thankful to Mrs. Margita Bodnárová for her skillful laboratory work. We also thank a lot for language revision and checking to Mr. Andrew Billingham.
Funding
The results presented here were funded by the Scientific Grant Agency of the Ministry of Education, Science research and Sport of the Slovak republic and the Slovak Academy of Sciences (Vega) within the project VEGA 2/0012/16.
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Approval in terms of animal ethics for the present study was obtained from the Ethics Committees of both institutions participating in the experimental application (Vega 2/0012/16).
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The authors declare that they have no competing interests.
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Lauková, A., Styková, E., Kubašová, I. et al. Enterocin M and its Beneficial Effects in Horses—a Pilot Experiment. Probiotics & Antimicro. Prot. 10, 420–426 (2018). https://doi.org/10.1007/s12602-018-9390-2
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DOI: https://doi.org/10.1007/s12602-018-9390-2