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Methods to Assess the Antioxidative Properties of Probiotics

Abstract

Probiotics prove useful in correcting and preventing numerous health conditions, including those having severe impact on society, e.g., obesity and cancer. Notably, these capabilities of probiotics appear to be associated with their antioxidant properties. The mechanisms of antioxidant action of probiotics range from immediate biochemical scavenging of reactive substances to induction of signaling events leading to increased capacity of the host’s cytoprotective systems. Since the antioxidant effects of probiotics significantly vary in types and details, a broad selection of methods of assessment of these properties is required in order to identify, characterize, and develop novel probiotics for medical purposes, as well as to explain the mechanisms of action of probiotics already in use in healthcare. This review revises the versatile toolbox, which can be used to assess the antioxidant properties of probiotics.

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References

  1. Prescott S, Nowak-Węgrzyn A (2011) Strategies to prevent or reduce allergic disease. Ann Nutr Metab 59(Suppl 1):28–42. https://doi.org/10.1159/000334150

    CAS  PubMed  Article  Google Scholar 

  2. Priebe MG, Vonk RJ, Sun X et al (2002) The physiology of colonic metabolism. Possibilities for interventions with pre-and probiotics. Eur J Nutr 41(Suppl 1):I2–I10

    PubMed  Google Scholar 

  3. Khansari N, Shakiba Y, Mahmoudi M (2009) Chronic inflammation and oxidative stress as a major cause of age-related diseases and cancer. Recent Patents Inflamm 3:73–80

    CAS  Google Scholar 

  4. Kadooka Y, Sato M, Imaizumi K, Ogawa A, Ikuyama K, Akai Y, Okano M, Kagoshima M, Tsuchida T (2010) Regulation of abdominal adiposity by probiotics (Lactobacillus gasseri SBT2055) in adults with obese tendencies in a randomized controlled trial. Eur J Clin Nutr 64(6):636–643. https://doi.org/10.1038/ejcn.2010.19

    CAS  PubMed  Article  Google Scholar 

  5. Vanderhoof JA, Mitmesser SH (2010) Probiotics in the management of children with allergy and other disorders of intestinal inflammation. Benefic Microbes 1(4):351–356. https://doi.org/10.3920/BM2010.0034

    CAS  Article  Google Scholar 

  6. Mishra V, Shah C, Mokashe N, Chavan R, Yadav H, Prajapati J (2015) Probiotics as potential antioxidants: a systematic review. J Agric Food Chem 63(14):3615–3626. https://doi.org/10.1021/jf506326t

    CAS  PubMed  Article  Google Scholar 

  7. Cenci G, Caldini G, Trotta F, Bosi P (2008) In vitro inhibitory activity of probiotic spore-forming bacilli against genotoxins. Lett Appl Microbiol 46(3):331–337. https://doi.org/10.1111/j.1472-765X.2007.02314.x

    CAS  PubMed  Article  Google Scholar 

  8. Cox CM, Dalloul RA (2014) Immunomodulatory role of probiotics in poultry and potential in ovo application. Benefic Microbes 6(1):45–52. https://doi.org/10.3920/BM2014.0062

    Article  Google Scholar 

  9. Floch MH (2014) Recommendations for probiotic use in humans - a 2014 update. Pharmaceuticals (Basel) 7(10):999–1007. https://doi.org/10.3390/ph7100999

    CAS  Article  Google Scholar 

  10. Achuthan AA, Duary RK, Madathil A, Panwar H, Kumar H, Batish VK, Grover S (2012) Antioxidative potential of lactobacilli isolated from the gut of Indian people. Mol Biol Rep 39(8):7887–7897. https://doi.org/10.1007/s11033-012-1633-9

    CAS  PubMed  Article  Google Scholar 

  11. Kullisaar T, Zilmer M, Mikelsaar M (2002) Two antioxidative lactobacilli strains as promising probiotics. Int J Food Microbiol 72(3):215–224. https://doi.org/10.1016/S0168-1605(01)00674-2

    CAS  PubMed  Article  Google Scholar 

  12. Mahdhi A, Leban N, Chakroun A et al (2017) Extracellular polysaccharide derived from potential probiotic strain with antioxidant and antibacterial activities as a prebiotic agent to control pathogenic bacterial biofilm formation. Microb Pathog 109:214–220. https://doi.org/10.1016/j.micpath.2017.05.046

    CAS  PubMed  Article  Google Scholar 

  13. Shen Q, Shang N, Li P (2011) In vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 isolated from centenarians. Curr Microbiol 62(4):1097–1103. https://doi.org/10.1007/s00284-010-9827-7

    CAS  PubMed  Article  Google Scholar 

  14. Wang Y, Wu Y, Wang Y et al (2017) Antioxidant properties of probiotic bacteria. Nutrients 9:E521. https://doi.org/10.3390/nu9050521

    CAS  PubMed  Article  Google Scholar 

  15. Chauhan R, Vasanthakumari AS, Panwar H et al (2014) Amelioration of colitis in mouse model by exploring antioxidative potentials of an indigenous probiotic strain of Lactobacillus fermentum Lf 1. Biomed Res Int 2014(206732):1–12. https://doi.org/10.1155/2014/206732

    Article  Google Scholar 

  16. Isolauri E, Kirjavainen PV, Salminen S (2002) Probiotics: a role in the treatment of intestinal infection and inflammation? Gut 50(suppl 3):iii54–iii59

    PubMed  PubMed Central  Google Scholar 

  17. Matsuu M, Shichijo K, Okaichi K et al (2003) The protective effect of fermented milk kefir on radiation-induced apoptosis in colonic crypt cells of rats. J Radiat Res 44(2):111–115. https://doi.org/10.1269/jrr.44.111

    PubMed  Article  Google Scholar 

  18. Stilling RM, Dinan TG, Cryan JF (2014) Microbial genes, brain and behaviour–epigenetic regulation of the gut-brain axis. Genes Brain Behav 13(1):69–86. https://doi.org/10.1111/gbb.12109

    CAS  PubMed  Article  Google Scholar 

  19. Renner HW, Münzner R (1991) The possible role of probiotics as dietary antimutagen. Mutat Res 262(4):239–245. https://doi.org/10.1016/0165-7992(91)90090-Q

    CAS  PubMed  Article  Google Scholar 

  20. Lo PR, RC Y, Chou CC et al (2004) Determinations of the antimutagenic activities of several probiotic bifidobacteria under acidic and bile conditions against benzo [a] pyrene by a modified Ames test. Int J Food Microbiol 93(2):249–257. https://doi.org/10.1016/j.ijfoodmicro.2003.11.008

    CAS  PubMed  Article  Google Scholar 

  21. Lopitz-Otsoa F, Rementeria A, Elguezabal N, Garaizar J (2006) Kefir: a symbiotic yeasts-bacteria community with alleged healthy capabilities. Rev Iberoam Micol 23(2):67–74. https://doi.org/10.1016/S1130-1406(06)70016-X

    PubMed  Article  Google Scholar 

  22. Gotteland M, Brunser O, Cruchet S (2006) Systematic review: are probiotics useful in controlling gastric colonization by Helicobacter pylori? Aliment Pharmacol Ther 23(8):1077–1086. https://doi.org/10.1111/j.1365-2036.2006.02868.x

    CAS  PubMed  Article  Google Scholar 

  23. Putignani L, Del Chierico F, Petrucca A, Vernocchi P, Dallapiccola B (2014) The human gut microbiota: a dynamic interplay with the host from birth to senescence settled during childhood. Pediatr Res 76(1):2–10. https://doi.org/10.1038/pr.2014.49

    PubMed  Article  Google Scholar 

  24. Zolotukhin P, Kozlova Y, Dovzhik A, Kovalenko K, Kutsyn K, Aleksandrova A, Shkurat T (2013) Oxidative status interactome map: towards novel approaches in experiment planning, data analysis, diagnostics and therapy. Mol BioSyst 9(8):2085–2096. https://doi.org/10.1039/c3mb70096h

    CAS  PubMed  Article  Google Scholar 

  25. Endo H, Niioka M, Kobayashi N, Tanaka M, Watanabe T (2013) Butyrate-producing probiotics reduce nonalcoholic fatty liver disease progression in rats: new insight into the probiotics for the gut-liver axis. PLoS One 8(5):e63388. https://doi.org/10.1371/journal.pone.0063388

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  26. Gao D, Gao Z, Zhu G (2013) Antioxidant effects of Lactobacillus plantarum via activation of transcription factor Nrf2. Food Funct 4(6):982–989. https://doi.org/10.1039/c3fo30316k

    CAS  PubMed  Article  Google Scholar 

  27. Lin YP, Thibodeaux CH, Peña JA, Ferry GD, Versalovic J (2008) Probiotic Lactobacillus reuteri suppress proinflammatory cytokines via c-Jun. Inflamm Bowel Dis 14(8):1068–1083. https://doi.org/10.1002/ibd.20448

    PubMed  Article  Google Scholar 

  28. Hegazy SK, El-Bedewy MM (2010) Effect of probiotics on pro-inflammatory cytokines and NF-κB activation in ulcerative colitis. World J Gastroenterol 16(33):4145–4151. https://doi.org/10.3748/wjg.v16.i33.4145

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  29. Karlsson M, Scherbak N, Khalaf H, Olsson PE, Jass J (2012) Substances released from probiotic Lactobacillus rhamnosus GR–1 potentiate NF-κB activity in Escherichia coli-stimulated urinary bladder cells. FEMS Immunol Med Microbiol 66(2):147–156. https://doi.org/10.1111/j.1574-695X.2012.00994.x

    CAS  PubMed  Article  Google Scholar 

  30. Wagner RD, Johnson SJ (2012) Probiotic lactobacillus and estrogen effects on vaginal epithelial gene expression responses to Candida albicans. J Biomed Sci 19:58. https://doi.org/10.1186/1423-0127-19-58

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  31. Wehkamp J, Harder J, Wehkamp K, Meissner BW, Schlee M, Enders C, Sonnenborn U, Nuding S, Bengmark S, Fellermann K, Schroder JM, Stange EF (2004) NF-kappaB- and AP-1-mediated induction of human beta defensin–2 in intestinal epithelial cells by Escherichia coli Nissle 1917: a novel effect of a probiotic bacterium. Infect Immun 72(10):5750–5758. https://doi.org/10.1128/IAI.72.10.5750-5758.2004

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  32. Schlee M, Wehkamp J, Altenhoefer A (2007) Induction of human beta-defensin 2 by the probiotic Escherichia coli Nissle 1917 is mediated through flagellin. Infect Immun 75(5):2399–2407. https://doi.org/10.1128/IAI.01563-06

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  33. Schlee M, Harder J, Köten B, Stange EF, Wehkamp J, Fellermann K (2008) Probiotic lactobacilli and VSL#3 induce enterocyte beta-defensin 2. Clin Exp Immunol 151(3):528–535. https://doi.org/10.1111/j.1365-2249.2007.03587.x

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  34. Balcerczyk A, Grzelak A, Janaszewska A, Jakubowski W, Koziol S, Marszalek M, Rychlik B, Soszynski M, Bilinski T, Bartosz G (2003) Thiols as major determinants of the total antioxidant capacity. Biofactors 17(1-4):75–82. https://doi.org/10.1002/biof.5520170108

    CAS  PubMed  Article  Google Scholar 

  35. Pinchuk I, Shoval H, Dotan Y, Lichtenberg D (2012) Evaluation of antioxidants: scope, limitations and relevance of assays. Chem Phys Lipids 165(6):638–647. https://doi.org/10.1016/j.chemphyslip.2012.05.003

    CAS  PubMed  Article  Google Scholar 

  36. Peluso I, Cavaliere A, Palmery M (2016) Plasma total antioxidant capacity and peroxidation biomarkers in psoriasis. J Biomed Sci 23(52):52. https://doi.org/10.1186/s12929-016-0268-x

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  37. Fraga CG, Oteiza PI, Galleano M (2014) In vitro measurements and interpretation of total antioxidant capacity. Biochim Biophys Acta 1840(2):931–934. https://doi.org/10.1016/j.bbagen.2013.06.030

    CAS  PubMed  Article  Google Scholar 

  38. Persichetti E, De Michele A, Codini M, Traina G (2014) Antioxidative capacity of Lactobacillus fermentum LF31 evaluated in vitro by oxygen radical absorbance capacity assay. Nutrition 30(7-8):936–938. https://doi.org/10.1016/j.nut.2013.12.009

    CAS  PubMed  Article  Google Scholar 

  39. Kavitha Rani PR, Fernandez A, George A, Remadevi VK, Sudarsanakumar MR, Laila SP, Arif M (2015) Synthesis, spectral characterization, molecular structure and pharmacological studies of N'-(1, 4-naphtho-quinone–2yl) isonicotinohydrazide. Spectrochim Acta A Mol Biomol Spectrosc 135:1156–1161. https://doi.org/10.1016/j.saa.2014.07.092

    CAS  PubMed  Article  Google Scholar 

  40. Amaretti A, di Nunzio M, Pompei A, Raimondi S, Rossi M, Bordoni A (2013) Antioxidant properties of potentially probiotic bacteria: in vitro and in vivo activities. Appl Microbiol Biotechnol 97(2):809–817. https://doi.org/10.1007/s00253-012-4241-7

    CAS  PubMed  Article  Google Scholar 

  41. Erel O (2004) A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 37(2):112–119. https://doi.org/10.1016/j.clinbiochem.2003.10.014

    CAS  PubMed  Article  Google Scholar 

  42. Gay C, Collins J, Gebicki JM (1999) Determination of iron in solutions with the ferric-xylenol orange complex. Anal Biochem 273(2):143–148. https://doi.org/10.1006/abio.1999.4207

    CAS  PubMed  Article  Google Scholar 

  43. Anwar H, Rahman ZU, Javed I, Muhammad F (2012) Effect of protein, probiotic, and symbiotic supplementation on serum biological health markers of molted layers. Poult Sci 91(10):2606–2613. https://doi.org/10.3382/ps.2012-02172

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  44. Mihara M, Uchiyama M (1978) Determination of malonaldehyde precursor in tissues by thiobarbituricacid test. Anal Biochem 86(1):271–278

    CAS  PubMed  Article  Google Scholar 

  45. Barbonetti A, Cinque B, Vassallo MR et al (2011) Effect of vaginal probiotic lactobacilli on in vitro-induced sperm lipid peroxidation and its impact on sperm motility and viability. Fertil Steril 95(8):2485–2488. https://doi.org/10.1016/j.fertnstert.2011.03.066

    CAS  PubMed  Article  Google Scholar 

  46. Czerska M, Mikołajewska K, Zieliński M, Gromadzińska J, Wąsowicz W (2015) Today’s oxidative stress markers. Med Pr 66(3):393–405. https://doi.org/10.13075/mp.5893.00137

    PubMed  Article  Google Scholar 

  47. Kullisaar T, Songisepp E, Mikelsaar M, Zilmer K, Vihalemm T, Zilmer M (2003) Antioxidative probiotic fermented goats’ milk decreases oxidative stress-mediated atherogenicity in human subjects. Br J Nutr 90(02):449–456. https://doi.org/10.1079/BJN2003896

    CAS  PubMed  Article  Google Scholar 

  48. He X, Slupsky CM, Dekker JW et al (2016) Integrated role of Bifidobacterium animalis subsp. lactis supplementation in gut microbiota, immunity, and metabolism of infant rhesus monkeys. mSystems 1

  49. Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47(3):469–474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x

    CAS  PubMed  Article  Google Scholar 

  50. Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I (1998) Study on the inhibitory effect of tannins and flavonoids against the 1,1-diphenyl–2 picrylhydrazyl radical. Biochem Pharmacol 56(2):213–222. https://doi.org/10.1016/S0006-2952(98)00128-2

    CAS  PubMed  Article  Google Scholar 

  51. Salma U, Miah AG, Tsujii H, Schellander K, Südekum KH (2012) Effect of dietary Rhodobacter capsulatus on lipid fractions and egg-yolk fatty acid composition in laying hens. J Anim Physiol Anim Nutr (Berl) 96(6):1091–1100. https://doi.org/10.1111/j.1439-0396.2011.01224.x

    CAS  Article  Google Scholar 

  52. Tang SG, Sieo CC, Kalavathy R et al (2015) Chemical compositions of egg yolks and egg quality of laying hens fed prebiotic, probiotic, and synbiotic diets. J Food Sci 80(8):C1686–C1695. https://doi.org/10.1111/1750-3841.12947

    CAS  PubMed  Article  Google Scholar 

  53. Nauseef WM (2014) Detection of superoxide anion and hydrogen peroxide production by cellular NADPH oxidases. Biochim Biophys Acta 1840(2):757–767. https://doi.org/10.1016/j.bbagen.2013.04.040

    CAS  PubMed  Article  Google Scholar 

  54. Wang L, Liu S, Zheng Z, Pi Z, Song F, Liu Z (2015) Rapid assay for testing superoxide anion radical scavenging activities to natural pigments by ultra-high performance liquid chromatography-diode-array detection method. Anal Methods 7(4):1535–1542. https://doi.org/10.1039/C4AY02690J

    CAS  Article  Google Scholar 

  55. Olojo RO, Xia RH, Abramson JJ (2005) Spectrophotometric and fluorometric assay of superoxide ion using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole. Anal Biochem 339(2):338–344. https://doi.org/10.1016/j.ab.2005.01.032

    CAS  PubMed  Article  Google Scholar 

  56. Hosoki K, Nakamura A, Nagao M, Hiraguchi Y, Tokuda R, Wada H, Nobori T, Fujisawa T (2010) Differential activation of eosinophils by 'probiotic' Bifidobacterium bifidum and ‘pathogenic’ Clostridium difficile. Int Arch Allergy Immunol 152(Suppl 1):83–89. https://doi.org/10.1159/000312131

    PubMed  Article  Google Scholar 

  57. Harikrishnan R, Balasundaram C, Heo MS (2010) Effect of probiotics enriched diet on Paralichthys olivaceus infected with lymphocystis disease virus (LCDV). Fish Shellfish Immunol 29(5):868–874. https://doi.org/10.1016/j.fsi.2010.07.031

    PubMed  Article  Google Scholar 

  58. Kodali VP, Sen R (2008) Antioxidant and free radical scavenging activities of an exopolysaccharide from a probiotic bacterium. Biotechnol J 3(2):245–251. https://doi.org/10.1002/biot.200700208

    CAS  PubMed  Article  Google Scholar 

  59. McBain AJ, Macfarlane GT (2001) Modulation of genotoxic enzyme activities by non-digestible oligosaccharide metabolism in in-vitro human gut bacterial ecosystems. J Med Microbiol 50(9):833–842. https://doi.org/10.1099/0022-1317-50-9-833

    CAS  PubMed  Article  Google Scholar 

  60. Kotova VY, Manukhov IV, Zavilgelskii GB (2010) Lux-biosensors for detection of SOS-response, heat shock, and oxidative stress. Appl Biochem Microbiol 46(8):781–788. https://doi.org/10.1134/S0003683810080089

    CAS  Article  Google Scholar 

  61. Su L, Jia W, Hou C, Lei Y (2011) Microbial biosensors: a review. Biosens Bioelectron 26(5):1788–1799. https://doi.org/10.1016/j.bios.2010.09.005

    CAS  PubMed  Article  Google Scholar 

  62. Grimoud J, Durand H, De Souza S et al (2010) In vitro screening of probiotics and synbiotics according to anti-inflammatory and anti-proliferative effects. Int J Food Microbiol 144(1):42–50. https://doi.org/10.1016/j.ijfoodmicro.2010.09.007

    CAS  PubMed  Article  Google Scholar 

  63. Prazdnova EV, Chistyakov VA, Churilov MN, Mazanko MS, Bren AB, Volski A, Chikindas ML (2015) DNA-protection and antioxidant properties of fermentates from Bacillus amyloliquefaciens B–1895 and Bacillus subtilis KATMIRA1933. Lett Appl Microbiol 61(6):549–554. https://doi.org/10.1111/lam.12491

    CAS  PubMed  Article  Google Scholar 

  64. Zavilgelsky GB, Kotova VY, Manukhov IV (2007) Action of 1,1-dimethylhydrazine on bacterial cells is determined by hydrogen peroxide. Mutat Res 634(1-2):172–176. https://doi.org/10.1016/j.mrgentox.2007.07.012

    CAS  PubMed  Article  Google Scholar 

  65. Farr SB, Kogoma T (1991) Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol Rev 55(4):561–585

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  66. Liochev SI, Hausladen A, Beyer WF, Fridovich I (1994) NADPH: ferredoxin oxidoreductase acts as a paraquatdiaphorase and is a member of the soxRS regulon. Proc Natl Acad Sci U S A 91(4):1328–1331. https://doi.org/10.1073/pnas.91.4.1328

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  67. Miller RL, Sun GY, Sun AY et al (2007) Cytotoxicity of paraquat in microglial cells: involvement of PKCδ-and ERK1/2-dependent NADPH oxidase. Brain Res 1167:129–139. https://doi.org/10.1016/j.brainres.2007.06.046

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  68. Chistyakov V, Melnikov V, Chikindas ML et al (2015) Poultry-beneficial solid-state Bacillus amyloliquefaciens B-1895 fermented soybean formulation. Biosci Microbiota Food Health 34(1):25–28. https://doi.org/10.12938/bmfh.2014-012

    CAS  PubMed  Article  Google Scholar 

  69. Karlyshev AV, Melnikov VG, Chistyakov VA (2014) Draft genome sequence of Bacillus amyloliquefaciens B-1895. Genome Announc 2(3):e00633–e00614. https://doi.org/10.1128/genomeA.00633-14

    PubMed  PubMed Central  Article  Google Scholar 

  70. Sutyak KE, Anderson RA, Dover SE, Feathergill KA, Aroutcheva AA, Faro S, Chikindas ML (2008) Spermicidal activity of the safe natural antimicrobial peptide subtilosin. Infect Dis Obstet Gynecol 2008:ID540758. https://doi.org/10.1155/2008/540758

    CAS  Article  Google Scholar 

  71. Amrouche T, Sutyak Noll K, Wang Y, Huang Q, Chikindas ML (2010) Antibacterial activity of subtilosin alone and combined with curcumin, poly-lysine and zinclactate against Listeria monocytogenes strains. Probiotics Antimicrob Proteins 2(4):250–257. https://doi.org/10.1007/s12602-010-9042-7

    CAS  PubMed  Article  Google Scholar 

  72. Sutyak KE, Wirawan RE, Aroutcheva AA, Chikindas ML (2008) Isolation of the Bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived Bacillus amyloliquefaciens. J Appl Microbiol 104(4):1067–1074. https://doi.org/10.1111/j.1365-2672.2007.03626.x

    CAS  PubMed  Article  Google Scholar 

  73. Noll KS, Sinko PJ, Chikindas ML (2011) Elucidation of the molecular mechanisms of action of the natural antimicrobial peptide subtilosin against the bacterial vaginosis-associated pathogen Gardnerella vaginalis. Probiotics Antimicrob Proteins 3(1):41–47. https://doi.org/10.1007/s12602-010-9061-4

    CAS  PubMed  PubMed Central  Article  Google Scholar 

  74. Guglielmetti S, Ciranna A, Mora D, Parini C, Karp M (2008) Construction, characterization and exemplificative application of bioluminescent Bifidobacterium longum biovar longum. Int J Food Microbiol 124(3):285–290. https://doi.org/10.1016/j.ijfoodmicro.2008.03.033

    CAS  PubMed  Article  Google Scholar 

  75. Abdhul K, Ganesh M, Shanmughapriya S, Kanagavel M, Anbarasu K, Natarajaseenivasan K (2014) Antioxidant activity of exopolysaccharide from probiotic strain Enterococcus faecium (BDU7) from Ngari. Int J BiolMacromol 70:450–454. https://doi.org/10.1016/j.ijbiomac.2014.07.026

    CAS  Article  Google Scholar 

  76. Balcázar JL, de Blas I, Ruiz-Zarzuela I et al (2007) Enhancement of the immune response and protection induced by probiotic lactic acid bacteria against furunculosis in rainbow trout (Oncorhynchus mykiss). FEMS Immunol Med Microbiol 51(1):185–193. https://doi.org/10.1111/j.1574-695X.2007.00294.x

    CAS  PubMed  Article  Google Scholar 

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Acknowledgements

The authors would like to thank Dr. Vijendra Mishra for critical reading and extremely helpful comments.

Funding

Preparation of this paper was supported by the grant of the Russian Science Foundation (Project No. 16-16-04032).

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Correspondence to E. V. Prazdnova.

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Zolotukhin, P.V., Prazdnova, E.V. & Chistyakov, V.A. Methods to Assess the Antioxidative Properties of Probiotics. Probiotics & Antimicro. Prot. 10, 589–599 (2018). https://doi.org/10.1007/s12602-017-9375-6

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Keywords

  • Antioxidant assays
  • Probiotics
  • Preventive healthcare
  • Biochemical scavenging
  • Signaling