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Evaluation of Probiotic Lactobacillus fermentum CCM 7421 Administration with Alginite in Dogs

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Abstract

There are growing efforts to find applications for various naturally occurring organo-mineral rocks. They have so far been preferentially used in agriculture and forestry; however, medicine and nutrition may also be interesting areas for their application. This study investigates the effects of dietary supplementation with canine-derived probiotic strain Lactobacillus fermentum CCM 7421 in combination with alginite in dogs. Alginite is a loam-like material of volcanic origin composed of clay minerals and fossilised unicellular algae. The effects of these additives on faecal microbiota, faecal characteristics, short-chain fatty acid profile, haematology, serum biochemistry and cellular immunity parameters were monitored. Forty dogs were randomly divided into four treatment groups: control group (C), alginite-supplemented group (A; 1% diet), probiotic group (LF; L. fermentum CCM 7421 at a dose of 109 cfu/day/dog) and combined group (A + LF group); 10 dogs in each group. The experiment lasted for 35 days with a 14-day treatment period (sample collection at days 0, 7, 14 and 35). The results of this straightforward experiment showed beneficial effects in the combined A + LF group. In detail, a decrease in faecal coliforms and clostridia and an increase in lactic acid bacteria, haemoglobin and serum magnesium levels compared to baseline were observed in the A + LF group (P < 0.05). In contrast, sole application of alginite (A group) led to several unexpected effects such as an increase in clostridial population and serum alanine aminotrasferase and a decrease in haemoglobin concentration (P < 0.05). The addition of alginite prevented a decrease in faecal pH and serum mineral content observed in the LF group. This indicates the possibility of applying alginite also in the nutrition of dogs as a combinative additive with probiotic bacteria for restoring optimal acid-alkali balance without affecting positive probiotic effects.

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Acknowledgements

We are most grateful to all the dog owners for their consent to the testing and their assistance during sampling procedures and to the company Algiwo s.r.o. for providing the alginite material.

Funding

The study was funded by the Slovak Scientific Agency VEGA (no. 2/0012/16 and no. 2/0056/13).

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Authors and Affiliations

  1. Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 040 01, Košice, Slovakia

    Viola Strompfová, Ivana Kubašová & Andrea Lauková

  2. Small Animal Clinic, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia

    Jana Farbáková & Aladár Maďari

  3. Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81, Košice, Slovakia

    Soňa Gancarčíková & Dagmar Mudroňová

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  1. Viola Strompfová
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  2. Ivana Kubašová
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Corresponding author

Correspondence to Viola Strompfová.

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Ethical Approval

All care and experimental procedures involving animals followed the guidelines stated in the Guide for the Care and Use of Animals approved by the State Veterinary and Food Administration of the Slovak Republic and by the Ethics Commission of the Institute of Animal Physiology, Slovak Academy of Sciences (no. 749/08-221). This article does not contain any studies with human participants performed by any of the authors.

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The authors declare that they have no conflict of interest.

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Strompfová, V., Kubašová, I., Farbáková, J. et al. Evaluation of Probiotic Lactobacillus fermentum CCM 7421 Administration with Alginite in Dogs. Probiotics & Antimicro. Prot. 10, 577–588 (2018). https://doi.org/10.1007/s12602-017-9370-y

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