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Colonization and Gut Flora Modulation of Lactobacillus kefiranofaciens ZW3 in the Intestinal Tract of Mice

Probiotics and Antimicrobial Proteins volume 10, pages 374–382 (2018)Cite this article

Abstract

This study evaluated the distribution and colonization of Lactobacillus kefiranofaciens ZW3 and determined its capacity to modulate the gut microbiota in an animal model. Based on (1) fluorescence imaging, (2) flow cytometry, and (3) qPCR, we found that ZW3 successfully adhered to mouse mucous tissue and colonized the mouse ileum. Gut microbiota profiling was performed using high-throughput sequencing. After continuous intubation with ZW3 for 1 week, the proportion of Lachnospiraceae, a family of butyric acid-producing bacteria, increased at day 7 (11.9% at day 0 versus 18.4% at day 7). In addition, Lactobacillaceae showed an increasing trend (4% at day 0 versus 13% at day 7) that was accompanied by an observable decline in the Rikenellaceae family (1.58% at day 7, 0.14% at day 14, and 0.75% at day 21) in the tested mouse. The results demonstrate that ZW3 could successfully adhere to and colonize the mouse gut throughout the course of the experiment. The profiling analysis of the gut microbiota also provided evidence supporting the function of ZW3 in improving the intestinal flora of mice.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant nos. 31171629 and 31401678).

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  1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Food Engineering and Biotechnology Institute, Tianjin University of Science and Technology, Tianjin, 300457, China

    Zhuqing Xing, Wei Tang, Ying Yang, Weitao Geng, Rizwan Ur Rehman & Yanping Wang

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  1. Zhuqing Xing
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Correspondence to Yanping Wang.

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Xing, Z., Tang, W., Yang, Y. et al. Colonization and Gut Flora Modulation of Lactobacillus kefiranofaciens ZW3 in the Intestinal Tract of Mice. Probiotics & Antimicro. Prot. 10, 374–382 (2018). https://doi.org/10.1007/s12602-017-9288-4

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Keywords

  • Lactobacillus kefiranofaciens ZW3
  • Distribution and colonization
  • High-throughput sequencing
  • Modulating the gut microbiota