Probiotics and Antimicrobial Proteins

, Volume 5, Issue 3, pp 176–186 | Cite as

Controlled Enzymatic Hydrolysis: A New Strategy for the Discovery of Antimicrobial Peptides

  • Estelle Yaba Adje
  • Rafik Balti
  • Didier Lecouturier
  • Mostafa Kouach
  • Pascal Dhulster
  • Didier Guillochon
  • Naïma Nedjar-Arroume
Article

Abstract

The use of antimicrobial peptides (AMPs) is an alternative to traditional antibiotics. AMPs are obtained using different methods such as bacterial synthesis, chemical synthesis and controlled enzymatic hydrolysis. The later is an interesting approach that deserves our attention because of the yields gathered and peptides engineered. Usually, activities of AMPs obtained in such a way are tightly dependent on the hydrolysis mechanism used. This paper deals with the hydrolysis of hemoglobin mechanism as a potential source of AMPs. Production of AMPs from hemoglobin using enzymatic controlled system is linked to hemoglobin structure. Further, we show that bovine hemoglobin, which is sensitive to peptic hydrolysis, results upon enzymatic digestion as a great source of AMPs. The hemoglobin in native and denatured states was hydrolyzed by “one-by-one” and “zipper” mechanisms, respectively. Nevertheless, a new mechanism named “semi-zipper” mechanism is obtained when protein is in molten globule structural state, constituting an original strategy for AMPs production. Seventy seven percentage of the peptides obtained by this new strategy showed antibacterial activity against nine strains.

Keywords

Antimicrobial peptides Hydrolysis mechanism Bovine hemoglobin Protein structure 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Estelle Yaba Adje
    • 1
  • Rafik Balti
    • 1
  • Didier Lecouturier
    • 1
  • Mostafa Kouach
    • 2
  • Pascal Dhulster
    • 1
  • Didier Guillochon
    • 1
  • Naïma Nedjar-Arroume
    • 1
  1. 1.Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), UPRES-EA 1026, Polytech’Lille/IUT «A»Université Lille I Sciences et TechnologiesVilleneuve d’Ascq CedexFrance
  2. 2.Laboratoire d’Application de Spectrométrie de Masse (LASM), Faculté des Sciences Pharmaceutiques et BiologiquesUniversité Lille II Droit et Santé, Service Commun de PhysicochimieLille CedexFrance

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