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A Novel Depsipeptide Produced by Paenibacillus alvei 32 Isolated from a Cystic fibrosis Patient

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Abstract

The important viscosity of the respiratory tract mucus of Cystic fibrosis (CF) patients impairs the mucociliary transport system and allows the growth of numerous micro-organisms. Among them, Pseudomonas aeruginosa and Staphylococcus aureus are known to be responsible for pulmonary infections. We imagined that CF microflora could also harbour micro-organisms naturally equipped to compete with these pathogens. A method was developed to recover these antibiotic-producing strains within 20 CF sputum. Using this approach, we have isolated an unusual Gram-positive bacterium identified as Paenibacillus alvei by Api galleries and 16S rRNA gene sequence analysis. This strain has inhibited the growth of P. aeruginosa, S. aureus and Klebsiella pneumoniae, in co-cultures. A liquid mineral medium named MODT50 was designed and optimised for the production and the recovery of the antimicrobial compounds. The supernatant has inhibited the growth of all Gram-positive strains tested, even Methicillin-resistant S. aureus. One antimicrobial compound with a peptide structure (mainly active against S. aureus, Micrococcus luteus, and Pseudomonas stutzeri) has been purified and characterised by liquid chromatography-mass spectrometry. The new active molecule (m/z 786.6) named depsipeptide l possesses a 15-guanidino-3-hydroxypentadecanoic acid side chain (m/z 298) linked on a cyclic part of four amino acids residues (Ser, two Leu/Ile, Arg). This work reports for the first time the production of such a molecule by a P. alvei strain in a mineral medium. The CF lung microflora might represent a valuable source for the discovery of new antimicrobial-producing strains.

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Acknowledgments

This study was supported by “l’Association Vaincre la Mucoviscidose”. The authors are indebted to the patients with CF from the CRCM of Nantes (France). We are grateful to Monique Bordes (CCA, La Rochelle, France) for her help with electron microscopy. We thank the Pr. Michael Givskov (Technical University of Denmark) for providing the PA01 lasB-gfp(ASV) strain. We thank Mr. Thomas Marsh for English corrections.

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Authors and Affiliations

  1. Laboratoire Littoral Environnement et Sociétés, LIENSs, Université de La Rochelle, UMR 7266, CNRS, Avenue Michel Crépeau, 17042, Rochelle Cedex 1, France

    Romain Chevrot, Sandrine Didelot, Fatoumata Tambadou, Pierre Marchand, Esther Izquierdo, Valérie Sopéna, Cyrille Barthélémy & Eric Rosenfeld

  2. Laboratoire ProBioGem, Université des Sciences et Technologies de Lille, 59655, Villeneuve d’Ascq Cedex, France

    Thibault Caradec

  3. Laboratoire de Thérapeutique Clinique & Expérimentale, Faculté de Médecine, UPRES EA 3826, 1 rue Gaston Veil, 44035, Nantes, France

    Jocelyne Caillon

  4. Faculteit Farmaceutische Wetenschappen, Laboratorium voor Farmaceutische Analyse, O&N 2, Herestraat 49, PB 923, 3000, Leuven, Belgium

    Larissa Van den Bossche, Ann Van Schepdael & Jos Hoogmartens

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  1. Romain Chevrot
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  2. Sandrine Didelot
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  3. Larissa Van den Bossche
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  4. Fatoumata Tambadou
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Corresponding authors

Correspondence to Romain Chevrot or Sandrine Didelot.

Additional information

Romain Chevrot and Sandrine Didelot contributed equally to this work.

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Chevrot, R., Didelot, S., Van den Bossche, L. et al. A Novel Depsipeptide Produced by Paenibacillus alvei 32 Isolated from a Cystic fibrosis Patient. Probiotics & Antimicro. Prot. 5, 18–25 (2013). https://doi.org/10.1007/s12602-012-9121-z

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  • DOI: https://doi.org/10.1007/s12602-012-9121-z

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