Probiotics and Antimicrobial Proteins

, Volume 5, Issue 1, pp 18–25 | Cite as

A Novel Depsipeptide Produced by Paenibacillus alvei 32 Isolated from a Cystic fibrosis Patient

  • Romain Chevrot
  • Sandrine Didelot
  • Larissa Van den Bossche
  • Fatoumata Tambadou
  • Thibault Caradec
  • Pierre Marchand
  • Esther Izquierdo
  • Valérie Sopéna
  • Jocelyne Caillon
  • Cyrille Barthélémy
  • Ann Van Schepdael
  • Jos Hoogmartens
  • Eric Rosenfeld


The important viscosity of the respiratory tract mucus of Cystic fibrosis (CF) patients impairs the mucociliary transport system and allows the growth of numerous micro-organisms. Among them, Pseudomonas aeruginosa and Staphylococcus aureus are known to be responsible for pulmonary infections. We imagined that CF microflora could also harbour micro-organisms naturally equipped to compete with these pathogens. A method was developed to recover these antibiotic-producing strains within 20 CF sputum. Using this approach, we have isolated an unusual Gram-positive bacterium identified as Paenibacillus alvei by Api galleries and 16S rRNA gene sequence analysis. This strain has inhibited the growth of P. aeruginosa, S. aureus and Klebsiella pneumoniae, in co-cultures. A liquid mineral medium named MODT50 was designed and optimised for the production and the recovery of the antimicrobial compounds. The supernatant has inhibited the growth of all Gram-positive strains tested, even Methicillin-resistant S. aureus. One antimicrobial compound with a peptide structure (mainly active against S. aureus, Micrococcus luteus, and Pseudomonas stutzeri) has been purified and characterised by liquid chromatography-mass spectrometry. The new active molecule (m/z 786.6) named depsipeptide l possesses a 15-guanidino-3-hydroxypentadecanoic acid side chain (m/z 298) linked on a cyclic part of four amino acids residues (Ser, two Leu/Ile, Arg). This work reports for the first time the production of such a molecule by a P. alvei strain in a mineral medium. The CF lung microflora might represent a valuable source for the discovery of new antimicrobial-producing strains.


Paenibacillus alvei Antimicrobial-peptide Cystic fibrosis Depsipeptide Fusaricidin 



This study was supported by “l’Association Vaincre la Mucoviscidose”. The authors are indebted to the patients with CF from the CRCM of Nantes (France). We are grateful to Monique Bordes (CCA, La Rochelle, France) for her help with electron microscopy. We thank the Pr. Michael Givskov (Technical University of Denmark) for providing the PA01 lasB-gfp(ASV) strain. We thank Mr. Thomas Marsh for English corrections.


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Romain Chevrot
    • 1
  • Sandrine Didelot
    • 1
  • Larissa Van den Bossche
    • 4
  • Fatoumata Tambadou
    • 1
  • Thibault Caradec
    • 2
  • Pierre Marchand
    • 1
  • Esther Izquierdo
    • 1
  • Valérie Sopéna
    • 1
  • Jocelyne Caillon
    • 3
  • Cyrille Barthélémy
    • 1
  • Ann Van Schepdael
    • 4
  • Jos Hoogmartens
    • 4
  • Eric Rosenfeld
    • 1
  1. 1.Laboratoire Littoral Environnement et Sociétés, LIENSsUniversité de La Rochelle, UMR 7266, CNRSRochelle Cedex 1France
  2. 2.Laboratoire ProBioGemUniversité des Sciences et Technologies de LilleVilleneuve d’Ascq CedexFrance
  3. 3.Laboratoire de Thérapeutique Clinique & Expérimentale, Faculté de MédecineUPRES EA 3826NantesFrance
  4. 4.Faculteit Farmaceutische WetenschappenLaboratorium voor Farmaceutische AnalyseLeuvenBelgium

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