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Ocean Science Journal

, Volume 54, Issue 2, pp 205–212 | Cite as

Effects of UV Radiation on Oxidative Stress in Yellowtail Clownfish Amphiprion clarkii

  • Han Seok Ryu
  • Cheol Young ChoiEmail author
  • Jin Ah Song
  • Gyung-Suk Kil
Article
  • 35 Downloads

Abstract

We investigated oxidative stress under UV radiation (380 nm) at two intensities (0.2 and 0.4 W/m2) for 14 days (0, 1, 3, 7 and 14) in the yellowtail clownfish Amphiprion clarkii. We analyzed mRNA expression and the activity of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], levels of plasma hydrogen peroxide (H2O2), lipid peroxide (LPO), alanine aminotransferase (AlaAT) and aspartate aminotransferase (AspAT). Furthermore, DNA damage was measured using comet assays and terminal transferase dUTP nick end labeling (TUNEL) assays were performed. As a result, the mRNA expressions and activities of antioxidant enzymes, the levels of plasma H2O2, LPO, AlaAT and AspAT were significantly higher in the group exposed to UV radiation than the control group. Finally, the comet and TUNEL assay showed that apoptosis was markedly higher at high UV intensity compared to the control group. These results indicate that UV radiation induces oxidative stress and decreases the antioxidant response in yellowtail clownfish.

Keywords

oxidative stress ultraviolet radiation yellowtail clownfish 

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Notes

Acknowledgments

This research was supported by the project titled ‘Development and commercialization of high density low temperature plasma based seawater sterilization purification system’ funded by the Ministry of Oceans and Fisheries, Korea.

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Copyright information

© Korea Institute of Ocean Science & Technology (KIOST) and the Korean Society of Oceanography (KSO) and Springer Science+Business Media B.V. 2019

Authors and Affiliations

  • Han Seok Ryu
    • 1
  • Cheol Young Choi
    • 1
    Email author
  • Jin Ah Song
    • 1
  • Gyung-Suk Kil
    • 2
  1. 1.Division of Marine BioScience, College of Ocean Science and TechnologyKorea Maritime and Ocean UniversityBusanKorea
  2. 2.Division of Electronics Engineering, College of EngineeringKorea Maritime and Ocean UniversityBusanKorea

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